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Science 3 August 1990: Vol. 249. no. 4968, pp. 533 - 537 DOI: 10.1126/science.2166335
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Articles
Science, Vol 249, Issue 4968, 533-537
Copyright © 1990 by American Association for the Advancement of Science
Identification of a sequence in the PEPCK gene that mediates a negative effect of insulin on transcription
RM O'Brien,
PC Lucas,
CD Forest,
MA Magnuson,
and
DK Granner
Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville, TN 37232-0615.
Phosphoenolpyruvate carboxykinase (PEPCK) governs the rate-limiting step in gluconeogenesis. Glucocorticoids and adenosine 3',5'-monophosphate (cAMP) increase PEPCK gene transcription and gluconeogenesis, whereas insulin has the opposite effect. Insulin is dominant, since it prevents cAMP and glucocorticoid-stimulated transcription. Glucocorticoid and cAMP response elements have been located in the PEPCK gene and now a 15-base pair insulin-responsive sequence (IRS) is described. Evidence for a binding activity that recognizes this sequence is presented.
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- M. Waltner-Law, M. C. Daniels, C. Sutherland, and D. K. Granner (2000)
J. Biol. Chem.
275, 31847-31856
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- Regulation of Phosphoenolpyruvate Carboxykinase and Insulin-like Growth Factor-binding Protein-1 Gene Expression by Insulin. THE ROLE OF WINGED HELIX/FORKHEAD PROTEINS.
- R. K. Hall, T. Yamasaki, T. Kucera, M. Waltner-Law, R. O'Brien, and D. K. Granner (2000)
J. Biol. Chem.
275, 30169-30175
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- A Nucleoprotein Complex containing CCAAT/Enhancer-binding Protein beta Interacts with an Insulin Response Sequence in the Insulin-like Growth Factor-binding Protein-1 Gene and Contributes to Insulin-regulated Gene Expression.
- A. K. Ghosh, R. Lacson, P. Liu, S. B. Cichy, A. Danilkovich, S. Guo, and T. G. Unterman (2001)
J. Biol. Chem.
276, 8507-8515
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- Role of Accessory Factors and Steroid Receptor Coactivator 1 in the Regulation of Phosphoenolpyruvate Carboxykinase Gene Transcription by Glucocorticoids.
- J. M. Stafford, M. Waltner-Law, and D. K. Granner (2001)
J. Biol. Chem.
276, 3811-3819
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- Gene- and Activation-specific Mechanisms for Insulin Inhibition of Basal and Glucocorticoid-induced Insulin-like Growth Factor Binding Protein-1 and Phosphoenolpyruvate Carboxykinase Transcription. ROLES OF FORKHEAD AND INSULIN RESPONSE SEQUENCES.
- D. Yeagley, S. Guo, T. Unterman, and P. G. Quinn (2001)
J. Biol. Chem.
276, 33705-33710
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- The Use of Transgenic Mice to Analyze the Role of Accessory Factor Two in the Regulation of Phosphoenolpyruvate Carboxykinase (GTP) Gene Transcription during Diabetes.
- P. S. Lechner, C. M. Croniger, P. Hakimi, C. Millward, C. Fekter, J. S. Yun, and R. W. Hanson (2001)
J. Biol. Chem.
276, 22675-22679
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- Sterol Regulatory Element-binding Protein-1c Mimics the Negative Effect of Insulin on Phosphoenolpyruvate Carboxykinase (GTP) Gene Transcription.
- K. Chakravarty, P. Leahy, D. Becard, P. Hakimi, M. Foretz, P. Ferre, F. Foufelle, and R. W. Hanson (2001)
J. Biol. Chem.
276, 34816-34823
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- Transducin-like Enhancer of Split Proteins, the Human Homologs of Drosophila Groucho, Interact with Hepatic Nuclear Factor 3beta.
- J.-C. Wang, M. Waltner-Law, K. Yamada, H. Osawa, S. Stifani, and D. K. Granner (2000)
J. Biol. Chem.
275, 18418-18423
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