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Science 27 July 1990:
Vol. 249. no. 4967, pp. 386 - 390
DOI: 10.1126/science.1696028

Articles

Science, Vol 249, Issue 4967, 386-390
Copyright © 1990 by American Association for the Advancement of Science


articles

Searching for peptide ligands with an epitope library

JK Scott and GP Smith

Division of Biological Sciences, University of Missouri, Columbia 65211.

Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.


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ATM-dependent phosphorylation of Mdm2 on serine 395: role in p53 activation by DNA damage.
R. Maya, M. Balass, S.-T. Kim, D. Shkedy, J.-F. M. Leal, O. Shifman, M. Moas, T. Buschmann, Z.'e. Ronai, Y. Shiloh, et al. (2001)
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Macromolecular Therapeutics: Emerging Strategies for Drug Discovery in the Postgenome Era.
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Human Monoclonal Rheumatoid Synovial B Lymphocyte Hybridoma with a New Disease-Related Specificity for Cartilage Oligomeric Matrix Protein.
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J. Immunol. 166, 4202-4208
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Identification of B-Cell Epitope of Dengue Virus Type 1 and Its Application in Diagnosis of Patients.
H.-C. Wu, Y.-L. Huang, T.-T. Chao, J.-T. Jan, J.-L. Huang, H.-Y. Chiang, C.-C. King, and M.-F. Shaio (2001)
J. Clin. Microbiol. 39, 977-982
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Epitope Mapping of trans-Sialidase from Trypanosoma cruzi Reveals the Presence of Several Cross-Reactive Determinants.
T. A. Pitcovsky, J. Mucci, P. Alvarez, M. S. Leguizamon, O. Burrone, P. M. Alzari, and O. Campetella (2001)
Infect. Immun. 69, 1869-1875
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Peptide mimicking antigenic and immunogenic epitope of double-stranded DNA in systemic lupus erythematosus.
Y. Sun, K.-Y. Fong, M. C. M. Chung, and Z.-J. Yao (2001)
Int. Immunol. 13, 223-232
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Rapid identification of a tobacco mosaic virus epitope by using a coat protein gene-fragment-pVIII fusion library.
A. Holzem, J. M. Nähring, and R. Fischer (2001)
J. Gen. Virol. 82, 9-15
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Identifying Diagnostic Peptides for Lyme Disease through Epitope Discovery.
G. A. Kouzmitcheva, V. A. Petrenko, and G. P. Smith (2001)
Clin. Vaccine Immunol. 8, 150-160
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Molecular Immunologic Strategies to Identify Antigens and B-Cell Responses Unique to Multiple Sclerosis.
D. H. Gilden, M. P. Burgoon, B. K. Kleinschmidt-DeMasters, R. A. Williamson, O. Ghausi, D. R. Burton, and G. P. Owens (2001)
Arch Neurol 58, 43-48
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Receptors for Hepatitis C Virus.
I. M. Jones, C. Chan-Fook, W.-R. Jiang, and B. E. Clarke (2000)
J. Virol. 74, 10860-10861
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Allergen mimotopes for 3-dimensional epitope search and induction of antibodies inhibiting human IgE.
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