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Science 13 July 1990:
Vol. 249. no. 4965, pp. 162 - 165
DOI: 10.1126/science.2115210
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Articles

Science, Vol 249, Issue 4965, 162-165
Copyright © 1990 by American Association for the Advancement of Science

articles

Identification of small clusters of divergent amino acids that mediate the opposing effects of ras and Krev-1

K Zhang, M Noda, WC Vass, AG Papageorge, and DR Lowy

Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, MD 20892.

Krev-1 is an anti-oncogene that was originally identified by its ability to induce morphologic reversion of ras-transformed cells that continue to express the ras gene. The Krev-1-encoded protein is structurally related to Ras proteins. The biological activities of a series of ras-Krev-1 chimeras were studied to test the hypothesis that Krev-1 may directly interfere with a ras function. The ras-specific and Krev-1-specific amino acids immediately surrounding residues 32 to 44, which are identical between the two proteins, determined whether the protein induced cellular transformation or suppressed ras transformation. Because this region in Ras proteins has been implicated in effector function, the results suggest that Krev-1 suppresses ras-induced transformation by interfering with interaction of Ras with its effector.


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Coassociation of Rap1A and Ha-Ras with Raf-1 N-terminal Region Interferes with Ras-dependent Activation of Raf-1.
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The Ras-related Protein Rheb Is Farnesylated and Antagonizes Ras Signaling and Transformation.
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A Constitutive Effector Region on the C-terminal Side of Switch I of the Ras Protein.
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Heterogeneous amino acids in Ras and Rap1A specifying sensitivity to GAP proteins.
K Zhang, A. Papageorge, P Martin, W. Vass, Z Olah, P. Polakis, F McCormick, and D. Lowy (1991)
Science 254, 1630-1634
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