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Science 29 June 1990:
Vol. 248. no. 4963, pp. 1650 - 1653
DOI: 10.1126/science.2194290
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Articles

Science, Vol 248, Issue 4963, 1650-1653
Copyright © 1990 by American Association for the Advancement of Science

articles

A cDNA for a protein that interacts with the human immunodeficiency virus Tat transactivator

P Nelbock, PJ Dillon, A Perkins, and CA Rosen

Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Hoffmann-La Roche Inc., Nutley, NJ 07110.

The human immunodeficiency virus (HIV) tat protein (Tat) is a positive regulator of virus gene expression and replication. Biotinylated Tat was used as a probe to screen a lambda gt11 fusion protein library, and a complementary DNA encoding a protein that interacts with Tat was cloned. Expression of this protein, designated TBP-1 (for Tat binding protein-1), was observed in a variety of cell lines, with expression being highest in human cells. TBP-1 was localized predominantly in the nucleus, which is consistent with the nuclear localization of Tat. In cotransfection experiments, expression of TBP-1 was able to specifically suppress Tat-mediated transactivation. The strategy described may be useful for direct identification and cloning of genes encoding proteins that associate with other proteins to modulate their activity in a positive or negative fashion.


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