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Science 22 June 1990:
Vol. 248. no. 4962, pp. 1541 - 1544
DOI: 10.1126/science.2163109
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Articles

Science, Vol 248, Issue 4962, 1541-1544
Copyright © 1990 by American Association for the Advancement of Science

articles

Molecular localization of the transforming and secretory properties of PDGF A and PDGF B

WJ LaRochelle, N Giese, M May-Siroff, KC Robbins, and SA Aaronson

Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

Human platelet-derived growth factor (PDGF) is a connective tissue cell mitogen comprised of two related chains encoded by distinct genes. The B chain is the homolog of the v-sis oncogene product. Properties that distinguish these ligands include greater transforming potency of the B chain and more efficient secretion of the A chain. By a strategy involving the generation of PDGF A and B chimeras, these properties were mapped to distinct domains of the respective molecules. Increased transforming efficiency segregated with the ability to activate both alpha and beta PDGF receptors. These findings genetically map PDGF B residues 105 to 144 as responsible for conformational alterations critical to beta PDGF receptor interaction and provide a mechanistic basis for the greater transforming potency of the PDGF B chain.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Platelet-derived Growth Factor (PDGF) Autocrine Signaling Regulates Survival and Mitogenic Pathways in Glioblastoma Cells: Evidence That the Novel PDGF-C and PDGF-D Ligands May Play a Role in the Development of Brain Tumors.
N. A. Lokker, C. M. Sullivan, S. J. Hollenbach, M. A. Israel, and N. A. Giese (2002)
Cancer Res. 62, 3729-3735
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Multiple Growth Factor Induction of a Murine Early Response Gene That Complements a Lethal Defect in Yeast Ribosome Biogenesis.
S. A. Nelson, J. P. Aris, B. K. R. Patel, and W. J. LaRochelle (2000)
J. Biol. Chem. 275, 13835-13841
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Hepatocyte Growth Factor (HGF)/NK1 Is a Naturally Occurring HGF/Scatter Factor Variant with Partial Agonist/Antagonist Activity.
V. Cioce, K. G. Csaky, A. M.-L. Chan, D. P. Bottaro, W. G. Taylor, R. Jensen, S. A. Aaronson, and J. S. Rubin (1996)
J. Biol. Chem. 271, 13110-13115
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The Intima : Soil for Atherosclerosis and Restenosis.
S. M. Schwartz, D. deBlois, and E. R. M. O'Brien (1995)
Circ. Res. 77, 445-465
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A novel mechanism regulating growth factor association with the cell surface: identification of a PDGF retention domain..
W J LaRochelle, M May-Siroff, K C Robbins, and S A Aaronson (1991)
Genes & Dev. 5, 1191-1199
   Abstract »    PDF »


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