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Science 25 May 1990: Vol. 248. no. 4958, pp. 1019 - 1023 DOI: 10.1126/science.2160731
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Articles
Science, Vol 248, Issue 4958, 1019-1023
Copyright © 1990 by American Association for the Advancement of Science
A receptor for tumor necrosis factor defines an unusual family of cellular and viral proteins
CA Smith,
T Davis,
D Anderson,
L Solam,
MP Beckmann,
R Jerzy,
SK Dower,
D Cosman,
and
RG Goodwin
Immunex Corporation, Seattle, WA 98101.
Tumor necrosis factor alpha and beta (TNF-alpha and TNF-beta) bind surface receptors on a variety of cell types to mediate a wide range of immunological responses, inflammatory reactions, and anti-tumor effects. A cDNA clone encoding an integral membrane protein of 461 amino acids was isolated from a human lung fibroblast library by direct expression screening with radiolabeled TNF-alpha. The encoded receptor was also able to bind TNF-beta. The predicted cysteine-rich extracellular domain has extensive sequence similarity with five proteins, including nerve growth factor receptor and a transcriptionally active open reading frame from Shope fibroma virus, and thus defines a family of receptors.
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