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Science 25 May 1990:
Vol. 248. no. 4958, pp. 1012 - 1016
DOI: 10.1126/science.2111580
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Articles
Science, Vol 248, Issue 4958, 1012-1016
Copyright © 1990 by American Association for the Advancement of Science
A novel nucleoprotein complex at a replication origin
M Serrano,
M Salas,
and
JM Hermoso
Centro de Biologia Molecular (CSIC-UAM), Universidad Autonoma, Madrid, Spain.
The viral protein p6, required for the protein-primed initiation of replication of Bacillus subtilis phage phi 29, forms a nucleoprotein complex at the viral replication origins that shows novel features. Deoxyribonuclease I and hydroxyl radical footprinting data, as well as the induction of positive supercoiling, support a model in which a DNA right-handed superhelix tightly wraps around a multimeric p6 core. The interaction occurs through the DNA minor groove. The activity of p6 not only requires the formation of the complex but also its correct positioning, indicating that the other proteins involved in the initiation of replication recognize, at a precise position, either the p6 core or the DNA conformational change induced by p6.
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