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Science 18 May 1990: Vol. 248. no. 4957, pp. 850 - 854 DOI: 10.1126/science.2188360
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Articles
Science, Vol 248, Issue 4957, 850-854
Copyright © 1990 by American Association for the Advancement of Science
Interaction of Hsp 70 with newly synthesized proteins: implications for protein folding and assembly
RP Beckmann,
LE Mizzen,
and
WJ Welch
Department of Medicine, University of California, San Francisco 94143.
The 70-kilodalton family of heat shock proteins (Hsp 70) has been implicated in posttranslational protein assembly and translocation. Binding of cytosolic forms of Hsp 70 (Hsp 72,73) with nascent proteins in the normal cell was investigated and found to be transient and adenosine triphosphate (ATP)-dependent. Interaction of Hsp 72,73 with newly synthesized proteins appeared to occur cotranslationally, because nascent polypeptides released prematurely from polysomes in vivo can be isolated in a complex with Hsp 72,73. Moreover, isolation of polysomes from short-term [35S]Met-labeled cells (pulsed) revealed that Hsp 72,73 associated with nascent polypeptide chains. In cells experiencing stress, newly synthesized proteins coimmunoprecipitated with Hsp 72,73; however, in contrast to normal cells, interaction with Hsp 72,73 was not transient. A model consistent with these data suggests that under normal growth conditions, cytosolic Hsp 72,73 interact transiently with nascent polypeptides to facilitate proper folding, and that metabolic stress interferes with these events.
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- GroEL Binds to and Unfolds Rhodanese Posttranslationally.
- B. G. Reid and G. C. Flynn (1996)
J. Biol. Chem.
271, 7212-7217
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- Phosphorylation of the Transit Sequence of Chloroplast Precursor Proteins.
- K. Waegemann and Jür. Soll (1996)
J. Biol. Chem.
271, 6545-6554
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- Activation of Heat Shock Factor by Alkylating Agents Is Triggered by Glutathione Depletion and Oxidation of Protein Thiols.
- H. Liu, R. Lightfoot, and J. L. Stevens (1996)
J. Biol. Chem.
271, 4805-4812
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- The speed of partial reactions of the uncoating ATPase Hsc70 depends on the source of coated vesicles.
- E. Buxbaum and P. G. Woodman (1996)
J. Cell Sci.
109, 705-711
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- Catalase Inhibition With 3-Amino-1,2,4-Triazole Does Not Abolish Infarct Size Reduction in Heat-Shocked Rats.
- Y. Auyeung, R. E. Sievers, D. Weng, V. Barbosa, and C. L. Wolfe (1995)
Circulation
92, 3318-3322
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- Structural Features of the Precursor to Mitochondrial Aspartate Aminotransferase Responsible for Binding to hsp70.
- B. Lain, A. Iriarte, J. R. Mattingly , Jr., J. I. Moreno, and M. Martinez-Carrion (1995)
J. Biol. Chem.
270, 24732-24739
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- Apoprotein B100, an Inefficiently Translocated Secretory Protein, Is Bound to the Cytosolic Chaperone, Heat Shock Protein 70.
- M. Zhou, X. Wu, L.-S. Huang, and H. N. Ginsberg (1995)
J. Biol. Chem.
270, 25220-25224
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- Polymerization of 70-kDa Heat Shock Protein by Yeast DnaJ in ATP.
- C. King, E. Eisenberg, and L. Greene (1995)
J. Biol. Chem.
270, 22535-22540
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- 70-kDa Heat Shock Cognate Protein Interacts Directly with the N-terminal Region of the Retinoblastoma Gene Product pRb.
- A. Inoue, T. Torigoe, K. Sogahata, K. Kamiguchi, S. Takahashi, Y. Sawada, M. Saijo, Y. Taya, S.-i. Ishii, N. Sato, et al. (1995)
J. Biol. Chem.
270, 22571-22576
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- Components of the Protein Synthesis and Folding Machinery Are Induced in Vascular Smooth Muscle Cells by Hypertrophic and Hyperplastic Agents.
- W. F. Patton, H. Erdjument-Bromage, A. R. Marks, P. Tempst, and M. B. Taubman (1995)
J. Biol. Chem.
270, 21404-21410
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- Heat Shock Enhances the Susceptibility of Tumor Cells to Lysis by Lymphokine-Activated Killer Cells.
- S. Fujieda, I. Noda, H. Saito, T. Hoshino, and M. Yagita (1995)
Arch Otolaryngol Head Neck Surg
121, 1009-1014
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- Divergent Effects of ATP on the Binding of the DnaK and DnaJ Chaperones to Each Other, or to Their Various Native and Denatured Protein Substrates.
- A. Wawrzynów and M. Zylicz (1995)
J. Biol. Chem.
270, 19300-19306
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- Calf Thymus Hsc70 Protein Protects and Reactivates Prokaryotic and Eukaryotic Enzymes.
- A. Ziemienowicz, M. Zylicz, C. Floth, and U. Hübscher (1995)
J. Biol. Chem.
270, 15479-15484
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- Accelerated recovery of postischemic stunned myocardium after induced expression of myocardial heat-shock protein (HSP70).
- B. L. Robinson, T. Morita, D. O. Toft, and J. J. Morris (1995)
J. Thorac. Cardiovasc. Surg.
109, 753-764
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- Effect of Nucleotide on the Binding of Peptides to 70-kDa Heat Shock Protein.
- L. E. Greene, R. Zinner, S. Naficy, and E. Eisenberg (1995)
J. Biol. Chem.
270, 2967-2973
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- Enzyme Assembly after de Novo Synthesis in Rabbit Reticulocyte Lysate Involves Molecular Chaperones and Immunophilins.
- M. Kruse, M. Brunke, A. Escher, A. A. Szalay, M. Tropschug, and R. Zimmermann (1995)
J. Biol. Chem.
270, 2588-2594
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- Heat shock protein (HSP 72) expression in patients undergoing cardiac operations.
- L. B. McGrath, M. Locke, M. Cane, C. Chen, and C. D. Ianuzzo (1995)
J. Thorac. Cardiovasc. Surg.
109, 370-376
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- Isoalloxazine Ring of FAD Is Required for the Formation of the Core in the Hsp60-assisted Folding of Medium Chain Acyl-CoA Dehydrogenase Subunit into the Assembly Competent Conformation in Mitochondria.
- T. Saijo and K. Tanaka (1995)
J. Biol. Chem.
270, 1899-1907
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