In Vivo Footprinting of a Muscle Specific Enhancer by Ligation Mediated PCR
Paul R. Mueller 1 and
Barbara Wold 1
1 Division of Biology, California Institute of Technology, 156-29, Pasadena, CA 91125
In the research artide "In vivo footprinting of a muscle specific enhancer by ligation mediated PCR" by P. R. Mueller and B. Wold (10 Nov. 1989, p. 780), an error was introduced after the galley proofs were approved by the authors. The ninth sentence of the legend for figure 2 contained an error in the concentration of deoxynudeoside triphosphate. It should have read, "Hybridization was stopped by transferring to ice; a solution of 7.5 µL of 20 mM MgCl2, 20 mM dithiothreitol (DTT), and 0.2 mM of each deoxynudeoside triphosphate (dNTP) was added...." The printed version read, "... 0.02 mM of each deoxynudeoside triphosphate (dNTP).. . ." This erroneous tenfold reduction in dNTP concentration may have a significant negative impact on the efficiency of the first strand synthesis reaction and therefore on the ultimate success of the ligation mediated PCR procedure.
