Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 11 May 1990:
Vol. 248. no. 4956, pp. 730 - 732
DOI: 10.1126/science.1970672
Prev | Table of Contents | Next

Articles

Science, Vol 248, Issue 4956, 730-732
Copyright © 1990 by American Association for the Advancement of Science

articles

Induction of Salmonella stress proteins upon infection of macrophages

NA Buchmeier and F Heffron

Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.

Regulated expression of bacterial genes allows a pathogen to adapt to new environmental conditions within the host. The synthesis of over 30 Salmonella proteins is selectively induced during infection of macrophages. Two proteins induced by Salmonella are the heat shock proteins GroEL and DnaK. Two avirulent, macrophage-sensitive mutants of Salmonella synthesize GroEL and DnaK but fail to synthesize different subsets of proteins normally induced within the macrophage. Enhanced expression of selected Salmonella proteins contributes to bacterial survival within macrophages and may also contribute to the apparent immunodominance of heat shock proteins.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
{sigma}32-Mediated Negative Regulation of Salmonella Pathogenicity Island 1 Expression.
M. Matsui, A. Takaya, and T. Yamamoto (2008)
J. Bacteriol. 190, 6636-6645
   Abstract »    Full Text »    PDF »
Coordinated Regulation of Expression of Salmonella Pathogenicity Island 1 and Flagellar Type III Secretion Systems by ATP-Dependent ClpXP Protease.
H. Kage, A. Takaya, M. Ohya, and T. Yamamoto (2008)
J. Bacteriol. 190, 2470-2478
   Abstract »    Full Text »    PDF »
Genome-Wide Screen of Salmonella Genes Expressed during Infection in Pigs, Using In Vivo Expression Technology.
Y. Huang, C. L. Leming, M. Suyemoto, and C. Altier (2007)
Appl. Envir. Microbiol. 73, 7522-7530
   Abstract »    Full Text »    PDF »
Identification of In Vivo-Expressed Immunogenic Proteins by Serological Proteome Analysis of the Bacillus anthracis Secretome.
T. Chitlaru, O. Gat, H. Grosfeld, I. Inbar, Y. Gozlan, and A. Shafferman (2007)
Infect. Immun. 75, 2841-2852
   Abstract »    Full Text »    PDF »
Identification of novel genes in genomic islands that contribute to Salmonella typhimurium replication in macrophages.
J. Klumpp and T. M. Fuchs (2007)
Microbiology 153, 1207-1220
   Abstract »    Full Text »    PDF »
Targeted Protein Degradation by Salmonella under Phagosome-mimicking Culture Conditions Investigated Using Comparative Peptidomics.
N. P. Manes, J. K. Gustin, J. Rue, H. M. Mottaz, S. O. Purvine, A. D. Norbeck, M. E. Monroe, J. S. D. Zimmer, T. O. Metz, J. N. Adkins, et al. (2007)
Mol. Cell. Proteomics 6, 717-727
   Abstract »    Full Text »    PDF »
Proteomic Analysis of Salmonella enterica Serovar Typhimurium Isolated from RAW 264.7 Macrophages: IDENTIFICATION OF A NOVEL PROTEIN THAT CONTRIBUTES TO THE REPLICATION OF SEROVAR TYPHIMURIUM INSIDE MACROPHAGES.
L. Shi, J. N. Adkins, J. R. Coleman, A. A. Schepmoes, A. Dohnkova, H. M. Mottaz, A. D. Norbeck, S. O. Purvine, N. P. Manes, H. S. Smallwood, et al. (2006)
J. Biol. Chem. 281, 29131-29140
   Abstract »    Full Text »    PDF »
Differential Proteomic Analysis of the Bacillus anthracis Secretome: Distinct Plasmid and Chromosome CO2-Dependent Cross Talk Mechanisms Modulate Extracellular Proteolytic Activities..
T. Chitlaru, O. Gat, Y. Gozlan, N. Ariel, and A. Shafferman (2006)
J. Bacteriol. 188, 3551-3571
   Abstract »    Full Text »    PDF »
Identification of Salmonella enterica Serovar Typhimurium Genes Important for Survival in the Swine Gastric Environment.
S. M. D. Bearson, B. L. Bearson, and M. A. Rasmussen (2006)
Appl. Envir. Microbiol. 72, 2829-2836
   Abstract »    Full Text »    PDF »
Absence of Evidence for the Participation of the Macrophage Cellular Prion Protein in Infection with Brucella suis.
P. Fontes, M.-T. Alvarez-Martinez, A. Gross, C. Carnaud, S. Kohler, and J.-P. Liautard (2005)
Infect. Immun. 73, 6229-6236
   Abstract »    Full Text »    PDF »
HtrA Stress Protein Is Involved in Intramacrophagic Replication of Adherent and Invasive Escherichia coli Strain LF82 Isolated from a Patient with Crohn's Disease.
M.-A. Bringer, N. Barnich, A.-L. Glasser, O. Bardot, and A. Darfeuille-Michaud (2005)
Infect. Immun. 73, 712-721
   Abstract »    Full Text »    PDF »
The ClpP Protease of Streptococcus pneumoniae Modulates Virulence Gene Expression and Protects against Fatal Pneumococcal Challenge.
H.-Y. Kwon, A. D. Ogunniyi, M.-H. Choi, S.-N. Pyo, D.-K. Rhee, and J. C. Paton (2004)
Infect. Immun. 72, 5646-5653
   Abstract »    Full Text »    PDF »
Bacterial and Host Factors Involved in the Major Histocompatibility Complex Class Ib-Restricted Presentation of Salmonella Hsp 60: Novel Pathway.
W.-F. Lo, C. D. Dunn, H. Ong, E. S. Metcalf, and M. J. Soloski (2004)
Infect. Immun. 72, 2843-2849
   Abstract »    Full Text »    PDF »
The DnaK/DnaJ Chaperone Machinery of Salmonella enterica Serovar Typhimurium Is Essential for Invasion of Epithelial Cells and Survival within Macrophages, Leading to Systemic Infection.
A. Takaya, T. Tomoyasu, H. Matsui, and T. Yamamoto (2004)
Infect. Immun. 72, 1364-1373
   Abstract »    Full Text »    PDF »
Effect of Heat Shock and Mutations in ClpL and ClpP on Virulence Gene Expression in Streptococcus pneumoniae.
H.-Y. Kwon, S.-W. Kim, M.-H. Choi, A. D. Ogunniyi, J. C. Paton, S.-H. Park, S.-N. Pyo, and D.-K. Rhee (2003)
Infect. Immun. 71, 3757-3765
   Abstract »    Full Text »    PDF »
Cross-Reactivity between Chlamydia trachomatis Heat Shock Protein 10 and Early Pregnancy Factor.
F. Betsou, M. J. Borrego, N. Guillaume, M. A. Catry, S. Romao, J. A. Machado-Caetano, J. M. Sueur, J. Mention, N. Faille, and J. Orfila (2003)
Clin. Vaccine Immunol. 10, 446-450
   Abstract »    Full Text »    PDF »
Transcriptional Analysis of Rickettsia prowazekii Invasion Gene Homolog (invA) during Host Cell Infection.
J. Gaywee, S. Radulovic, J. A. Higgins, and A. F. Azad (2002)
Infect. Immun. 70, 6346-6354
   Abstract »    Full Text »    PDF »
Differential Regulation of Multiple Proteins of Escherichia coli and Salmonella enterica Serovar Typhimurium by the Transcriptional Regulator SlyA.
A. Spory, A. Bosserhoff, C. von Rhein, W. Goebel, and A. Ludwig (2002)
J. Bacteriol. 184, 3549-3559
   Abstract »    Full Text »    PDF »
Induction of dnaK through Its Native Heat Shock Promoter Is Necessary for Intramacrophagic Replication of Brucella suis.
S. Kohler, E. Ekaza, J.-Y. Paquet, K. Walravens, J. Teyssier, J. Godfroid, and J.-P. Liautard (2002)
Infect. Immun. 70, 1631-1634
   Abstract »    Full Text »    PDF »
The Extracytoplasmic Sigma Factor, {varsigma}E, Is Required for Intracellular Survival of Nontypeable Haemophilus influenzae in J774 Macrophages.
J. E. Craig, A. Nobbs, and N. J. High (2002)
Infect. Immun. 70, 708-715
   Abstract »    Full Text »    PDF »
In Vivo Genetic Analysis Indicates That PhoP-PhoQ and the Salmonella Pathogenicity Island 2 Type III Secretion System Contribute Independently to Salmonella enterica Serovar Typhimurium Virulence.
C. R. Beuzon, K. E. Unsworth, and D. W. Holden (2001)
Infect. Immun. 69, 7254-7261
   Abstract »    Full Text »    PDF »
Molecular Cloning and Sequencing of the Circumsporozoite Protein Gene from Plasmodium falciparum Strain FCC-1/HN and Expression of the Gene in Mycobacteria.
C. Zheng, P. Xie, and Y. Chen (2001)
J. Clin. Microbiol. 39, 2911-2915
   Abstract »    Full Text »    PDF »
Listeria Pathogenesis and Molecular Virulence Determinants.
J. A. Vazquez-Boland, M. Kuhn, P. Berche, T. Chakraborty, G. Dominguez-Bernal, W. Goebel, B. Gonzalez-Zorn, J. Wehland, and J. Kreft (2001)
Clin. Microbiol. Rev. 14, 584-640
   Abstract »    Full Text »    PDF »
Characterization of the groESL Operon in Listeria monocytogenes: Utilization of Two Reporter Systems (gfp and hly) for Evaluating In Vivo Expression.
C. G. M. Gahan, J. O'Mahony, and C. Hill (2001)
Infect. Immun. 69, 3924-3932
   Abstract »    Full Text »    PDF »
Disruption of the Genes for ClpXP Protease in Salmonella enterica Serovar Typhimurium Results in Persistent Infection in Mice, and Development of Persistence Requires Endogenous Gamma Interferon and Tumor Necrosis Factor Alpha.
T. Yamamoto, H. Sashinami, A. Takaya, T. Tomoyasu, H. Matsui, Y. Kikuchi, T. Hanawa, S. Kamiya, and A. Nakane (2001)
Infect. Immun. 69, 3164-3174
   Abstract »    Full Text »    PDF »
Differential expression of mycobacterial proteins following phagocytosis by macrophages.
I. M. Monahan, J. Betts, D. K. Banerjee, and P. D. Butcher (2001)
Microbiology 147, 459-471
   Abstract »    Full Text »
Salmonella enterica Serovar Typhimurium-Induced Maturation of Bone Marrow-Derived Dendritic Cells.
M. Svensson, C. Johansson, and M. J. Wick (2000)
Infect. Immun. 68, 6311-6320
   Abstract »    Full Text »    PDF »
Host Immune Response to Intracellular Bacteria: A Role for MHC-Linked Class-Ib Antigen-Presenting Molecules.
M. J. Soloski, M. E. Szperka, A. Davies, and S. L. Wooden (2000)
Experimental Biology and Medicine 224, 231-239
   Abstract »    Full Text »
Regulation of Brucella abortus Catalase.
J.-a Kim, Z. Sha, and J. E. Mayfield (2000)
Infect. Immun. 68, 3861-3866
   Abstract »    Full Text »    PDF »
Characterization of Heat, Oxidative, and Acid Stress Responses in Brucella melitensis.
A. P. Teixeira-Gomes, A. Cloeckaert, and M. S. Zygmunt (2000)
Infect. Immun. 68, 2954-2961
   Abstract »    Full Text »    PDF »
OmpR Regulates the Two-Component System SsrA-SsrB in Salmonella Pathogenicity Island 2.
A. K. Lee, C. S. Detweiler, and S. Falkow (2000)
J. Bacteriol. 182, 771-781
   Abstract »    Full Text »
Salmonella typhimurium Virulence Genes Are Induced upon Bacterial Invasion into Phagocytic and Nonphagocytic Cells.
C. G. Pfeifer, S. L. Marcus, O. Steele-Mortimer, L. A. Knodler, and B. B. Finlay (1999)
Infect. Immun. 67, 5690-5698
   Abstract »    Full Text »    PDF »
Attenuation of virulence in Mycobacterium tuberculosis expressing a constitutively active iron repressor.
Y. C. Manabe, B. J. Saviola, L. Sun, J. R. Murphy, and W. R. Bishai (1999)
PNAS 96, 12844-12848
   Abstract »    Full Text »    PDF »
Humoral and Cellular Immune Responses in Mice Immunized with Recombinant Mycobacterium bovis Bacillus Calmette-Guerin Producing a Pertussis Toxin-Tetanus Toxin Hybrid Protein.
B. Abomoelak, K. Huygen, L. Kremer, M. Turneer, and C. Locht (1999)
Infect. Immun. 67, 5100-5105
   Abstract »    Full Text »    PDF »
T Cell Responses to Gram-Negative Intracellular Bacterial Pathogens: A Role for CD8+ T Cells in Immunity to Salmonella Infection and the Involvement of MHC Class Ib Molecules.
W.-F. Lo, H. Ong, E. S. Metcalf, and M. J. Soloski (1999)
J. Immunol. 162, 5398-5406
   Abstract »    Full Text »    PDF »
Role of Heat Shock Proteins in Protection from and Pathogenesis of Infectious Diseases.
U. Zugel and S. H. E. Kaufmann (1999)
Clin. Microbiol. Rev. 12, 19-39
   Abstract »    Full Text »    PDF »
Identification of a 71-Kilodalton Surface-Associated Hsp70 Homologue in Coxiella burnetii.
A. Macellaro, E. Tujulin, K. Hjalmarsson, and L. Norlander (1998)
Infect. Immun. 66, 5882-5888
   Abstract »    Full Text »    PDF »
Characterization of the Thermal Stress Response of Campylobacter jejuni.
M. E. Konkel, B. J. Kim, J. D. Klena, C. R. Young, and R. Ziprin (1998)
Infect. Immun. 66, 3666-3672
   Abstract »    Full Text »    PDF »
Enhanced Levels of Staphylococcus aureus Stress Protein GroEL and DnaK Homologs Early in Infection of Human Epithelial Cells.
M. W. Qoronfleh, C. A. Bortner, P. Schwartzberg, and B. J. Wilkinson (1998)
Infect. Immun. 66, 3024-3027
   Abstract »    Full Text »    PDF »
GroEL Heat Shock Protein of Haemophilus ducreyi: Association with Cell Surface and Capacity To Bind to Eukaryotic Cells.
A. Frisk, C. A. Ison, and T. Lagergard (1998)
Infect. Immun. 66, 1252-1257
   Abstract »    Full Text »    PDF »
Cell-Specific Proteins Synthesized by Salmonella typhimurium.
L. Burns-Keliher, C. A. Nickerson, B. J. Morrow, and R. Curtiss III (1998)
Infect. Immun. 66, 856-861
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)