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Science 4 May 1990:
Vol. 248. no. 4955, pp. 591 - 593
DOI: 10.1126/science.2110382
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Articles

Science, Vol 248, Issue 4955, 591-593
Copyright © 1990 by American Association for the Advancement of Science

articles

In vivo receptor-mediated phosphorylation of a G protein in Dictyostelium

RE Gundersen and PN Devreotes

Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Extracellular adenosine 3',5'-monophosphate (cAMP) serves multiple roles in Dictyostelium development, acting as a chemoattractant, a cell-cell signaling molecule, and an inducer of differentiation. The Dictyostelium G-protein alpha subunit G alpha 2 appears to be the major transducer linking the surface cAMP receptor to these intracellular responses. On stimulation of cells with cAMP, G alpha 2 is phosphorylated on one or more serine residues, resulting in an alteration of its electrophoretic mobility. Phosphorylation of G alpha 2 is triggered by increased occupancy of the surface cAMP receptor and is rapid and transient, coinciding with the time course of activation of physiological responses.


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