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Science 4 May 1990:
Vol. 248. no. 4955, pp. 585 - 588
DOI: 10.1126/science.2185542
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Articles

Science, Vol 248, Issue 4955, 585-588
Copyright © 1990 by American Association for the Advancement of Science

articles

A Mn2(+)-dependent ribozyme

V Dange, RB Van Atta, and SM Hecht

Department of Chemistry, University of Virginia, Charlottesville 22901.

An RNA hairpin identical in sequence with the one formed during autocyclization of the 414-nucleotide Tetrahymena intervening sequence undergoes strand scission at a specific site in the presence of Mn2+. In addition to representing one of the smallest and simplest ribozymes possible, strand scission occurs readily under physiological conditions, is unaffected by the presence of Mg2+, and displays salt, pH, and temperature optima of potential use in exploiting Mn2+ as a regulatory switch in intact cells. The chemistry of strand scission of the RNA hairpin is described, as is the Mn2(+)-dependent solvolysis of a 231-nucleotide RNA transcript containing this structural motif.


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