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Science 27 April 1990:
Vol. 248. no. 4954, pp. 486 - 490
DOI: 10.1126/science.1970441
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Articles

Science, Vol 248, Issue 4954, 486-490
Copyright © 1990 by American Association for the Advancement of Science

articles

A bacterial enhancer functions to tether a transcriptional activator near a promoter

A Wedel, DS Weiss, D Popham, P Droge, and S Kustu

Department of Plant Pathology, University of California, Berkeley 94720.

The nitrogen regulatory protein NtrC of enteric bacteria activates transcription of the glnA gene by catalyzing isomerization of closed complexes between RNA polymerase and the glnA promoter to open complexes. NtrC binds to sites upstream of glnA that have properties of eukaryotic transcriptional enhancers. NtrC-binding sites were found to facilitate open complex formation when these sites and the glnA promoter were located on different rings of a singly linked catenane, but not when the two rings were decatenated. The results provide evidence that NtrC contacts RNA polymerase-promoter complexes in a process mediated by formation of a DNA loop. NtrC-binding sites serve to tether NtrC near the glnA promoter, thereby increasing the frequency of collisions between NtrC and polymerase-promoter complexes.


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