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Science 13 April 1990:
Vol. 248. no. 4952, pp. 223 - 226
DOI: 10.1126/science.2109351
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Articles

Science, Vol 248, Issue 4952, 223-226
Copyright © 1990 by American Association for the Advancement of Science

articles

A promoter that drives transgene expression in cerebellar Purkinje and retinal bipolar neurons

J Oberdick, RJ Smeyne, Mann JR, S Zackson, and JI Morgan

Department of Neuroscience, Roche Research Center, Nutley, NJ 07110.

A genomic clone encoding the Purkinje cell-specific L7 protein has been isolated and utilized to drive the expression of beta-galactosidase in mice. Three independent transgenic lines, germ line transformed with an L7-beta-galactosidase fusion gene, exhibit beta-galactosidase expression in both cerebellar Purkinje cells and retinal bipolar neurons. This distribution is the same as that previously determined for the L7 protein by immunohistochemistry. The transgenic murine lines can be used to obtain populations of marked Purkinje and bipolar neurons. Similar L7 promoter constructs can be used to express other foreign genes specifically in these two classes of neurons.


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