Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 13 April 1990:
Vol. 248. no. 4952, pp. 201 - 204
DOI: 10.1126/science.2326633
Prev | Table of Contents | Next

Articles

Science, Vol 248, Issue 4952, 201-204
Copyright © 1990 by American Association for the Advancement of Science

articles

Primary sequence information from intact proteins by electrospray ionization tandem mass spectrometry

JA Loo, CG Edmonds, and RD Smith

Chemical Sciences Department, Pacific Northwest Laboratory, Richland, WA 99352.

Tandem mass spectrometry has been used to obtain information related to portions of the primary sequence for an intact protein, bovine ribonuclease A. Multiply charged molecular ions, generated by electrospray ionization, were collisionally dissociated at low energies in a triple quadrupole mass spectrometer to yield singly and multiply charged fragment ions that can be assigned to the known sequence of the protein. Dissociation of the highly charged molecular ions resulted in pairs of complementary product ions. The higher order (gas-phase) protein structure affects the dissociation processes, as observed in comparisons of tandem mass spectra of the native and disulfide-reduced forms of ribonuclease A.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification of membrane proteins by tandem mass spectrometry of protein ions.
J. Carroll, M. C. Altman, I. M. Fearnley, and J. E. Walker (2007)
PNAS 104, 14330-14335
   Abstract »    Full Text »    PDF »
Extending top-down mass spectrometry to proteins with masses greater than 200 kilodaltons..
X. Han, M. Jin, K. Breuker, and F. W. McLafferty (2006)
Science 314, 109-112
   Abstract »    Full Text »    PDF »
The Escherichia coli Proteome: Past, Present, and Future Prospects.
M.-J. Han and S. Y. Lee (2006)
Microbiol. Mol. Biol. Rev. 70, 362-439
   Abstract »    Full Text »    PDF »
High Level Oxacillin and Vancomycin Resistance and Altered Cell Wall Composition in Staphylococcus aureus Carrying the Staphylococcal mecA and the Enterococcal vanA Gene Complex.
A. Severin, K. Tabei, F. Tenover, M. Chung, N. Clarke, and A. Tomasz (2004)
J. Biol. Chem. 279, 3398-3407
   Abstract »    Full Text »    PDF »
The Catalytic Subunit of the cAMP-dependent Protein Kinase of Ovine Sperm Flagella Has a Unique Amino-terminal Sequence.
J. T. S. Agustin, J. D. Leszyk, L. M. Nuwaysir, and G. B. Witman (1998)
J. Biol. Chem. 273, 24874-24883
   Abstract »    Full Text »    PDF »
Characterization of Adult alpha - and beta -Globin Genes in the Zebrafish.
F.-Y. Chan, J. Robinson, A. Brownlie, R. A. Shivdasani, A. Donovan, C. Brugnara, J. Kim, B.-C. Lau, H. E. Witkowska, and L. I. Zon (1997)
Blood 89, 688-700
   Abstract »    Full Text »    PDF »
Mass spectrometry and its use in tandem with laser spectroscopy.
E. Grant and R. Cooks (1990)
Science 250, 61-68
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)