Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Science Signaling - Call For Papers

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 6 April 1990:
Vol. 248. no. 4951, pp. 73 - 76
DOI: 10.1126/science.2321027
Prev | Table of Contents | Next

Articles

Science, Vol 248, Issue 4951, 73-76
Copyright © 1990 by American Association for the Advancement of Science

articles

Two-photon laser scanning fluorescence microscopy

W Denk, JH Strickler, and WW Webb

School of Applied and Engineering Physics, Department of Physics, Cornell University, Ithaca, NY 14853.

Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption in the ultraviolet with a stream of strongly focused subpicosecond pulses of red laser light has made possible fluorescence images of living cells and other microscopic objects. The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation. This technique also provides unprecedented capabilities for three-dimensional, spatially resolved photochemistry, particularly photolytic release of caged effector molecules.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Fluorescence Changes of Genetic Calcium Indicators and OGB-1 Correlated with Neural Activity and Calcium In Vivo and In Vitro.
T. Hendel, M. Mank, B. Schnell, O. Griesbeck, A. Borst, and D. F. Reiff (2008)
J. Neurosci. 28, 7399-7411
   Abstract »    Full Text »    PDF »
Functional Stability of Retinal Ganglion Cells after Degeneration-Induced Changes in Synaptic Input.
D. J. Margolis, G. Newkirk, T. Euler, and P. B. Detwiler (2008)
J. Neurosci. 28, 6526-6536
   Abstract »    Full Text »    PDF »
I5S: Wide-Field Light Microscopy with 100-nm-Scale Resolution in Three Dimensions.
L. Shao, B. Isaac, S. Uzawa, D. A. Agard, J. W. Sedat, and M. G. L. Gustafsson (2008)
Biophys. J. 94, 4971-4983
   Abstract »    Full Text »    PDF »
Optical Lock-In Detection of FRET Using Synthetic and Genetically Encoded Optical Switches.
S. Mao, R. K. P. Benninger, Y. Yan, C. Petchprayoon, D. Jackson, C. J. Easley, D. W. Piston, and G. Marriott (2008)
Biophys. J. 94, 4515-4524
   Abstract »    Full Text »    PDF »
In vivo two-photon voltage-sensitive dye imaging reveals top-down control of cortical layers 1 and 2 during wakefulness.
B. Kuhn, W. Denk, and R. M. Bruno (2008)
PNAS 105, 7588-7593
   Abstract »    Full Text »    PDF »
Fluorescence Interferometry: Principles and Applications in Biology.
A. BILENCA, J. CAO, M. COLICE, A. OZCAN, B. BOUMA, L. RAFTERY, and G. TEARNEY (2008)
Ann. N.Y. Acad. Sci. 1130, 68-77
   Abstract »    Full Text »    PDF »
Two-photon Excitation Fluorescence Bioassays.
P. HANNINEN, J. SOUKKA, and J. T. SOINI (2008)
Ann. N.Y. Acad. Sci. 1130, 320-326
   Abstract »    Full Text »    PDF »
Dendritic Excitability and Synaptic Plasticity.
P. J. Sjostrom, E. A. Rancz, A. Roth, and M. Hausser (2008)
Physiol Rev 88, 769-840
   Abstract »    Full Text »    PDF »
Livin' on the Edge: Imaging Dendritic Spine Turnover in the Peri-Infarct Zone during Ischemic Stroke and Recovery.
C. E. Brown and T. H. Murphy (2008)
Neuroscientist 14, 139-146
   Abstract »    PDF »
Nonlinear Integration of Binocular Optic Flow by DNOVS2, A Descending Neuron of the Fly.
A. Wertz, A. Borst, and J. Haag (2008)
J. Neurosci. 28, 3131-3140
   Abstract »    Full Text »    PDF »
FCS Imaging--A Way to Look at Cellular Processes.
Z. Gryczynski (2008)
Biophys. J. 94, 1943-1944
   Full Text »    PDF »
Two-Photon Excitation of Potentiometric Probes Enables Optical Recording of Action Potentials From Mammalian Nerve Terminals In Situ.
J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras, and B. M. Salzberg (2008)
J Neurophysiol 99, 1545-1553
   Abstract »    Full Text »    PDF »
Enhanced Background Rejection in Thick Tissue with Differential-Aberration Two-Photon Microscopy.
A. Leray, K. Lillis, and J. Mertz (2008)
Biophys. J. 94, 1449-1458
   Abstract »    Full Text »    PDF »
The Application of Fluorescent Quantum Dots to Confocal, Multiphoton, and Electron Microscopic Imaging.
T. J. Deerinck (2008)
Toxicol Pathol 36, 112-116
   Abstract »    Full Text »    PDF »
Long-Term, High-Resolution Imaging in the Neocortex In Vivo.
B. E. Chen, J. T. Trachtenberg, A. J.G.D. Holtmaat, and K. Svoboda (2008)
CSH Protocols 2008, pdb.prot4902
   Abstract »    Full Text »
In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia.
M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam (2007)
PNAS 104, 19494-19499
   Abstract »    Full Text »    PDF »
In Vivo Calcium Imaging of Neural Network Function.
W. Gobel and F. Helmchen (2007)
Physiology 22, 358-365
   Abstract »    Full Text »    PDF »
New Angles on Neuronal Dendrites In Vivo.
W. Gobel and F. Helmchen (2007)
J Neurophysiol 98, 3770-3779
   Abstract »    Full Text »    PDF »
Serotonin Modulates Dendritic Calcium Influx in Commissural Interneurons in the Mouse Spinal Locomotor Network.
M. Diaz-Rios, D. A. Dombeck, W. W. Webb, and R. M. Harris-Warrick (2007)
J Neurophysiol 98, 2157-2167
   Abstract »    Full Text »    PDF »
The Power of Single and Multibeam Two-Photon Microscopy for High-Resolution and High-Speed Deep Tissue and Intravital Imaging.
R. Niesner, V. Andresen, J. Neumann, H. Spiecker, and M. Gunzer (2007)
Biophys. J. 93, 2519-2529
   Abstract »    Full Text »    PDF »
Principles of Multiphoton-Excitation Fluorescence Microscopy.
W. Denk (2007)
CSH Protocols 2007, pdb.top23
   Abstract »    Full Text »
The Role of Backscattering in SHG Tissue Imaging.
F. Legare, C. Pfeffer, and B. R. Olsen (2007)
Biophys. J. 93, 1312-1320
   Abstract »    Full Text »    PDF »
Rat liver sinusoidal endothelial cells survive without exogenous VEGF in 3D perfused co-cultures with hepatocytes.
A. J. Hwa, R. C. Fry, A. Sivaraman, P. T. So, L. D. Samson, D. B. Stolz, and L. G. Griffith (2007)
FASEB J 21, 2564-2579
   Abstract »    Full Text »    PDF »
Spectrally Resolved Multiphoton Imaging of In Vivo and Excised Mouse Skin Tissues.
J. A. Palero, H. S. de Bruijn, A. van der Ploeg van den Heuvel, H. J. C. M. Sterenborg, and H. C. Gerritsen (2007)
Biophys. J. 93, 992-1007
   Abstract »    Full Text »    PDF »
Protracted Synaptogenesis after Activity-Dependent Spinogenesis in Hippocampal Neurons.
U. V. Nagerl, G. Kostinger, J. C. Anderson, K. A. C. Martin, and T. Bonhoeffer (2007)
J. Neurosci. 27, 8149-8156
   Abstract »    Full Text »    PDF »
Long-Term Two-Photon Transcranial Imaging of Synaptic Structures in the Living Brain.
J. Grutzendler and W.-B. Gan (2007)
CSH Protocols 2007, pdb.prot4766
   Abstract »    Full Text »
Requirements for T Lymphocyte Migration in Explanted Lymph Nodes.
J. H. Huang, L. I. Cardenas-Navia, C. C. Caldwell, T. J. Plumb, C. G. Radu, P. N. Rocha, T. Wilder, J. S. Bromberg, B. N. Cronstein, M. Sitkovsky, et al. (2007)
J. Immunol. 178, 7747-7755
   Abstract »    Full Text »    PDF »
The Relationship between Blood Flow and Neuronal Activity in the Rodent Olfactory Bulb.
E. Chaigneau, P. Tiret, J. Lecoq, M. Ducros, T. Knopfel, and S. Charpak (2007)
J. Neurosci. 27, 6452-6460
   Abstract »    Full Text »    PDF »
Robust coding of flow-field parameters by axo-axonal gap junctions between fly visual interneurons.
H. Cuntz, J. Haag, F. Forstner, I. Segev, and A. Borst (2007)
PNAS 104, 10229-10233
   Abstract »    Full Text »    PDF »
Live imaging of collagen remodeling during angiogenesis.
N. D. Kirkpatrick, S. Andreou, J. B. Hoying, and U. Utzinger (2007)
Am J Physiol Heart Circ Physiol 292, H3198-H3206
   Abstract »    Full Text »    PDF »
Different Mechanisms Generate Maintained Activity in ON and OFF Retinal Ganglion Cells.
D. J. Margolis and P. B. Detwiler (2007)
J. Neurosci. 27, 5994-6005
   Abstract »    Full Text »    PDF »
Far-Field Optical Nanoscopy.
S. W. Hell (2007)
Science 316, 1153-1158
   Abstract »    Full Text »    PDF »
Second Harmonic and Sum Frequency Generation Imaging of Fibrous Astroglial Filaments in Ex Vivo Spinal Tissues.
Y. Fu, H. Wang, R. Shi, and J.-X. Cheng (2007)
Biophys. J. 92, 3251-3259
   Abstract »    Full Text »    PDF »
Two-Photon Calcium Imaging of Network Activity in XFP-Expressing Neurons in the Mouse.
J. M. Wilson, D. A. Dombeck, M. Diaz-Rios, R. M. Harris-Warrick, and R. M. Brownstone (2007)
J Neurophysiol 97, 3118-3125
   Abstract »    Full Text »    PDF »
Integration of Lobula Plate Output Signals by DNOVS1, an Identified Premotor Descending Neuron.
J. Haag, A. Wertz, and A. Borst (2007)
J. Neurosci. 27, 1992-2000
   Abstract »    Full Text »    PDF »
Hormonal Regulation of Nuclear Permeability.
E. M. O'Brien, D. A. Gomes, S. Sehgal, and M. H. Nathanson (2007)
J. Biol. Chem. 282, 4210-4217
   Abstract »    Full Text »    PDF »
Optical Recording of Action Potentials and Other Discrete Physiological Events: A Perspective from Signal Detection Theory.
L. Sjulson and G. Miesenbock (2007)
Physiology 22, 47-55
   Abstract »    Full Text »    PDF »
Multiphoton Fluorescence and Second Harmonic Generation Imaging of the Structural Alterations in Keratoconus Ex Vivo.
H.-Y. Tan, Y. Sun, W. Lo, S.-J. Lin, C.-H. Hsiao, Y.-F. Chen, S. C.-M. Huang, W.-C. Lin, S.-H. Jee, H.-S. Yu, et al. (2006)
Invest. Ophthalmol. Vis. Sci. 47, 5251-5259
   Abstract »    Full Text »    PDF »
Dual-Color Photon Counting Histogram Analysis of mRFP1 and EGFP in Living Cells.
L. N. Hillesheim, Y. Chen, and J. D. Muller (2006)
Biophys. J. 91, 4273-4284
   Abstract »    Full Text »    PDF »
Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing.
M. Rueckel, J. A. Mack-Bucher, and W. Denk (2006)
PNAS 103, 17137-17142
   Abstract »    Full Text »    PDF »
Spine Ca2+ Signaling in Spike-Timing-Dependent Plasticity.
T. Nevian and B. Sakmann (2006)
J. Neurosci. 26, 11001-11013
   Abstract »    Full Text »    PDF »
Exploring Mitochondria in the Intact Ischemic Heart: Advancing Technologies to Image Intracellular Function.
M. N. Sack (2006)
Circulation 114, 1452-1454
   Full Text »    PDF »
Real-Time 2-Photon Imaging of Mitochondrial Function in Perfused Rat Hearts Subjected to Ischemia/Reperfusion.
M. Matsumoto-Ida, M. Akao, T. Takeda, M. Kato, and T. Kita (2006)
Circulation 114, 1497-1503
   Abstract »    Full Text »    PDF »
Aqueous humor outflow: what do we know? Where will it lead us?.
M. P. Fautsch, D. H. Johnson, and the Second ARVO/Pfizer Research Institute Working (2006)
Invest. Ophthalmol. Vis. Sci. 47, 4181-4187
   Full Text »    PDF »
Two-Photon-Excited Fluorescence Imaging of Human RPE Cells with a Femtosecond Ti:Sapphire Laser..
A. Bindewald-Wittich, M. Han, S. Schmitz-Valckenberg, S. R. Snyder, G. Giese, J. F. Bille, and F. G. Holz (2006)
Invest. Ophthalmol. Vis. Sci. 47, 4553-4557
   Abstract »    Full Text »    PDF »
Dual-Color Time-Integrated Fluorescence Cumulant Analysis.
B. Wu, Y. Chen, and J. D. Muller (2006)
Biophys. J. 91, 2687-2698
   Abstract »    Full Text »    PDF »
Nuclear Localization Signal Receptor Affinity Correlates with in Vivo Localization in Saccharomyces cerevisiae.
A. E. Hodel, M. T. Harreman, K. F. Pulliam, M. E. Harben, J. S. Holmes, M. R. Hodel, K. M. Berland, and A. H. Corbett (2006)
J. Biol. Chem. 281, 23545-23556
   Abstract »    Full Text »    PDF »
Macromolecular-scale resolution in biological fluorescence microscopy.
G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Luhrmann, R. Jahn, C. Eggeling, and S. W. Hell (2006)
PNAS 103, 11440-11445
   Abstract »    Full Text »    PDF »
Nonperturbative Chemical Imaging of Organelle Transport in Living Cells with Coherent Anti-Stokes Raman Scattering Microscopy.
X. Nan, E. O. Potma, and X. S. Xie (2006)
Biophys. J. 91, 728-735
   Abstract »    Full Text »    PDF »
Calcium Release Domains in Mammalian Skeletal Muscle Studied with Two-photon Imaging and Spot Detection Techniques.
J. Gomez, P. Neco, M. DiFranco, and J. L. Vergara (2006)
J. Gen. Physiol. 127, 623-637
   Abstract »    Full Text »    PDF »
Fanciful FRET.
S. S. Vogel, C. Thaler, and S. V. Koushik (2006)
Sci. STKE 2006, re2
   Abstract »    Full Text »    PDF »
Living cells as test tubes..
X. S. Xie, J. Yu, and W. Y. Yang (2006)
Science 312, 228-230
   Abstract »    Full Text »    PDF »
Approaches for biological and biomimetic energy conversion.
D. A. LaVan and J. N. Cha (2006)
PNAS 103, 5251-5255
   Abstract »    Full Text »    PDF »
Multiphoton autofluorescence and second-harmonic generation imaging of the ex vivo porcine eye..
S.-W. Teng, H.-Y. Tan, J.-L. Peng, H.-H. Lin, K. H. Kim, W. Lo, Y. Sun, W.-C. Lin, S.-J. Lin, S.-H. Jee, et al. (2006)
Invest. Ophthalmol. Vis. Sci. 47, 1216-1224
   Abstract »    Full Text »    PDF »
Overcoming photodamage in second-harmonic generation microscopy: Real-time optical recording of neuronal action potentials.
L. Sacconi, D. A. Dombeck, and W. W. Webb (2006)
PNAS 103, 3124-3129
   Abstract »    Full Text »    PDF »
Fast Functional Imaging of Single Neurons Using Random-Access Multiphoton (RAMP) Microscopy.
V. Iyer, T. M. Hoogland, and P. Saggau (2006)
J Neurophysiol 95, 535-545
   Abstract »    Full Text »    PDF »
Quantitative 3D fluorescence technique for the analysis of en face preparations of arterial walls using quantum dot nanocrystals and two-photon excitation laser scanning microscopy.
D. E. Ferrara, D. Weiss, P. H. Carnell, R. P. Vito, D. Vega, X. Gao, S. Nie, and W. R. Taylor (2006)
Am J Physiol Regulatory Integrative Comp Physiol 290, R114-R123
   Abstract »    Full Text »    PDF »
Diffusion and scaling during early embryonic pattern formation.
T. Gregor, W. Bialek, R. R. d. R. van Steveninck, D. W. Tank, and E. F. Wieschaus (2005)
PNAS 102, 18403-18407
   Abstract »    Full Text »    PDF »
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.
C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie (2005)
PNAS 102, 16807-16812
   Abstract »    Full Text »    PDF »
Temporally resolved interactions between antigen-stimulated IgE receptors and Lyn kinase on living cells.
D. R. Larson, J. A. Gosse, D. A. Holowka, B. A. Baird, and W. W. Webb (2005)
J. Cell Biol. 171, 527-536
   Abstract »    Full Text »    PDF »
Map Plasticity in Somatosensory Cortex.
D. E. Feldman and M. Brecht (2005)
Science 310, 810-815
   Abstract »    Full Text »    PDF »
Optical Recording of Fast Neuronal Membrane Potential Transients in Acute Mammalian Brain Slices by Second-Harmonic Generation Microscopy.
D. A. Dombeck, L. Sacconi, M. Blanchard-Desce, and W. W. Webb (2005)
J Neurophysiol 94, 3628-3636
   Abstract »    Full Text »    PDF »
A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets.
H. Kasai, H. Hatakeyama, T. Kishimoto, T.-T. Liu, T. Nemoto, and N. Takahashi (2005)
J. Physiol. 568, 891-903
   Abstract »    Full Text »    PDF »
Quantitative intravital microscopy using a Generalized Polarity concept for kidney studies.
W. Yu, R. M. Sandoval, and B. A. Molitoris (2005)
Am J Physiol Cell Physiol 289, C1197-C1208
   Abstract »    Full Text »    PDF »
Symposium Presentations.
V. Fuster (2005)
J. Am. Coll. Cardiol. 46, 5A-70A
   Full Text »    PDF »
Imaging input and output dynamics of neocortical networks in vivo: Exciting times ahead.
A. Grinvald (2005)
PNAS 102, 14125-14126
   Full Text »    PDF »
Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution.
M. G. L. Gustafsson (2005)
PNAS 102, 13081-13086
   Abstract »    Full Text »    PDF »
Observation Volumes and {gamma}-Factors in Two-Photon Fluorescence Fluctuation Spectroscopy.
A. Nagy, J. Wu, and K. M. Berland (2005)
Biophys. J. 89, 2077-2090
   Abstract »    Full Text »    PDF »
Donor Islet Endothelial Cells Participate in Formation of Functional Vessels Within Pancreatic Islet Grafts.
D. Nyqvist, M. Kohler, H. Wahlstedt, and P.-O. Berggren (2005)
Diabetes 54, 2287-2293
   Abstract »    Full Text »    PDF »
Two-Photon Activation and Excitation Properties of PA-GFP in the 720-920-nm Region.
M. Schneider, S. Barozzi, I. Testa, M. Faretta, and A. Diaspro (2005)
Biophys. J. 89, 1346-1352
   Abstract »    Full Text »    PDF »
Coherent Anti-Stokes Raman Scattering Imaging of Axonal Myelin in Live Spinal Tissues.
H. Wang, Y. Fu, P. Zickmund, R. Shi, and J.-X. Cheng (2005)
Biophys. J. 89, 581-591
   Abstract »    Full Text »    PDF »
Two-Photon Excitation in Fluorescence Polarization Receptor-Ligand Binding Assay.
M. E. Tirri, R. J. Huttunen, J. Toivonen, P. L. Harkonen, J. T. Soini, and P. E. Hanninen (2005)
J Biomol Screen 10, 314-319
   Abstract »    PDF »
Multiphoton imaging of renal tissues in vitro.
J. Peti-Peterdi (2005)
Am J Physiol Renal Physiol 288, F1079-F1083
   Abstract »    Full Text »    PDF »
Unraveling Protein-Protein Interactions in Living Cells with Fluorescence Fluctuation Brightness Analysis.
Y. Chen, L.-N. Wei, and J. D. Muller (2005)
Biophys. J. 88, 4366-4377
   Abstract »    Full Text »    PDF »
In Vivo Performance of Genetically Encoded Indicators of Neural Activity in Flies.
D. F. Reiff, A. Ihring, G. Guerrero, E. Y. Isacoff, M. Joesch, J. Nakai, and A. Borst (2005)
J. Neurosci. 25, 4766-4778
   Abstract »    Full Text »    PDF »
Target Cell-Dependent Normalization of Transmitter Release at Neocortical Synapses.
H. J. Koester and D. Johnston (2005)
Science 308, 863-866
   Abstract »    Full Text »    PDF »
Sphingosine 1-phosphate induces cytoskeletal reorganization in C2C12 myoblasts: physiological relevance for stress fibres in the modulation of ion current through stretch-activated channels.
L. Formigli, E. Meacci, C. Sassoli, F. Chellini, R. Giannini, F. Quercioli, B. Tiribilli, R. Squecco, P. Bruni, F. Francini, et al. (2005)
J. Cell Sci. 118, 1161-1171
   Abstract »    Full Text »    PDF »
Skeletal Muscle NAD(P)H Two-Photon Fluorescence Microscopy In Vivo: Topology and Optical Inner Filters.
E. C. Rothstein, S. Carroll, C. A. Combs, P. D. Jobsis, and R. S. Balaban (2005)
Biophys. J. 88, 2165-2176
   Abstract »    Full Text »    PDF »
Dual-Color Photon-Counting Histogram.
Y. Chen, M. Tekmen, L. Hillesheim, J. Skinner, B. Wu, and J. D. Muller (2005)
Biophys. J. 88, 2177-2192
   Abstract »    Full Text »    PDF »
Multiphoton Microscopy of Endogenous Fluorescence Differentiates Normal, Precancerous, and Cancerous Squamous Epithelial Tissues.
M. C. Skala, J. M. Squirrell, K. M. Vrotsos, J. C. Eickhoff, A. Gendron-Fitzpatrick, K. W. Eliceiri, and N. Ramanujam (2005)
Cancer Res. 65, 1180-1186
   Abstract »    Full Text »    PDF »
Dynamic Multiphoton Imaging: A Live View from Cells to Systems.
G. E. Stutzmann and I. Parker (2005)
Physiology 20, 15-21
   Abstract »    Full Text »    PDF »
Interpreting Second-Harmonic Generation Images of Collagen I Fibrils.
R. M. Williams, W. R. Zipfel, and W. W. Webb (2005)
Biophys. J. 88, 1377-1386
   Abstract »    Full Text »    PDF »
In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses.
W. Supatto, D. Debarre, B. Moulia, E. Brouzes, J.-L. Martin, E. Farge, and E. Beaurepaire (2005)
PNAS 102, 1047-1052
   Abstract »    Full Text »    PDF »
Two-Photon Microscopy of Cells and Tissue.
M. Rubart (2004)
Circ. Res. 95, 1154-1166
   Abstract »    Full Text »    PDF »
Use of coherent control methods through scattering biological tissue to achieve functional imaging.
J. M. Dela Cruz, I. Pastirk, M. Comstock, V. V. Lozovoy, and M. Dantus (2004)
PNAS 101, 16996-17001
   Abstract »    Full Text »    PDF »
Cooperative 4Pi Excitation and Detection Yields Sevenfold Sharper Optical Sections in Live-Cell Microscopy.
H. Gugel, J. Bewersdorf, S. Jakobs, J. Engelhardt, R. Storz, and S. W. Hell (2004)
Biophys. J. 87, 4146-4152
   Abstract »    Full Text »    PDF »
A Genetic Switch for Epilepsy in Adult Mice.
H. E. Krestel, D. R. Shimshek, V. Jensen, T. Nevian, J. Kim, Y. Geng, T. Bast, A. Depaulis, K. Schonig, F. Schwenk, et al. (2004)
J. Neurosci. 24, 10568-10578
   Abstract »    Full Text »    PDF »
Guiding neuronal development with in situ microfabrication.
B. Kaehr, R. Allen, D. J. Javier, J. Currie, and J. B. Shear (2004)
PNAS 101, 16104-16108
   Abstract »    Full Text »    PDF »
Fly motion vision is based on Reichardt detectors regardless of the signal-to-noise ratio.
J. Haag, W. Denk, and A. Borst (2004)
PNAS 101, 16333-16338
   Abstract »    Full Text »    PDF »
Novel Approaches to Monitor and Manipulate Single Neurons In Vivo.
M. Brecht, M. S. Fee, O. Garaschuk, F. Helmchen, T. W. Margrie, K. Svoboda, and P. Osten (2004)
J. Neurosci. 24, 9223-9227
   Full Text »    PDF »
Imaging Coronary Artery Microstructure Using Second-Harmonic and Two-Photon Fluorescence Microscopy.
A. Zoumi, X. Lu, G. S. Kassab, and B. J. Tromberg (2004)
Biophys. J. 87, 2778-2786
   Abstract »    Full Text »    PDF »
Stabilization of Exocytosis by Dynamic F-actin Coating of Zymogen Granules in Pancreatic Acini.
T. Nemoto, T. Kojima, A. Oshima, H. Bito, and H. Kasai (2004)
J. Biol. Chem. 279, 37544-37550
   Abstract »    Full Text »    PDF »