Related Content
Search Google Scholar for:
|
|
Science 6 April 1990: Vol. 248. no. 4951, pp. 73 - 76 DOI: 10.1126/science.2321027
|
|
Articles
Science, Vol 248, Issue 4951, 73-76
Copyright © 1990 by American Association for the Advancement of Science
Two-photon laser scanning fluorescence microscopy
W Denk,
JH Strickler,
and
WW Webb
School of Applied and Engineering Physics, Department of Physics, Cornell University, Ithaca, NY 14853.
Molecular excitation by the simultaneous absorption of two photons provides intrinsic three-dimensional resolution in laser scanning fluorescence microscopy. The excitation of fluorophores having single-photon absorption in the ultraviolet with a stream of strongly focused subpicosecond pulses of red laser light has made possible fluorescence images of living cells and other microscopic objects. The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation. This technique also provides unprecedented capabilities for three-dimensional, spatially resolved photochemistry, particularly photolytic release of caged effector molecules.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Improved in vivo two-photon imaging after blood replacement by perfluorocarbon.
- F. Haiss, R. Jolivet, M. T. Wyss, J. Reichold, N. B. Braham, F. Scheffold, M. P. Krafft, and B. Weber (2009)
J. Physiol.
587, 3153-3158
| Abstract »
| Full Text »
| PDF »
- Independent two-photon measurements of albumin GSC give low values.
- J. Peti-Peterdi (2009)
Am J Physiol Renal Physiol
296, F1255-F1257
| Full Text »
| PDF »
- Connectivity Patterns Revealed by Mapping of Active Inputs on Dendrites of Thalamorecipient Neurons in the Auditory Cortex.
- R. J. Richardson, J. A. Blundon, I. T. Bayazitov, and S. S. Zakharenko (2009)
J. Neurosci.
29, 6406-6417
| Abstract »
| Full Text »
| PDF »
- Multiphoton Imaging of Renal Regulatory Mechanisms.
- J. Peti-Peterdi, I. Toma, A. Sipos, and S. L. Vargas (2009)
Physiology
24, 88-96
| Abstract »
| Full Text »
| PDF »
- Electrical Coupling of Lobula Plate Tangential Cells to a Heterolateral Motion-Sensitive Neuron in the Fly.
- J. Haag and A. Borst (2008)
J. Neurosci.
28, 14435-14442
| Abstract »
| Full Text »
| PDF »
- Multilayer three-dimensional super resolution imaging of thick biological samples.
- A. Vaziri, J. Tang, H. Shroff, and C. V. Shank (2008)
PNAS
105, 20221-20226
| Abstract »
| Full Text »
| PDF »
- Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy.
- C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie (2008)
Science
322, 1857-1861
| Abstract »
| Full Text »
| PDF »
- Live-cell imaging of dendritic spines by STED microscopy.
- U. V. Nagerl, K. I. Willig, B. Hein, S. W. Hell, and T. Bonhoeffer (2008)
PNAS
105, 18982-18987
| Abstract »
| Full Text »
| PDF »
- What Can Population Calcium Imaging Tell Us About Neural Circuits?.
- A. C. Kwan (2008)
J Neurophysiol
100, 2977-2980
| Abstract »
| Full Text »
| PDF »
- Dissecting the Components of Long-Term Potentiation.
- J. A. Blundon and S. S. Zakharenko (2008)
Neuroscientist
14, 598-608
| Abstract »
| PDF »
- Detection of Corneal Fibrosis by Imaging Second Harmonic-Generated Signals in Rabbit Corneas Treated with Mitomycin C after Excimer Laser Surface Ablation.
- M. Farid, N. Morishige, L. Lam, A. Wahlert, R. F. Steinert, and J. V. Jester (2008)
Invest. Ophthalmol. Vis. Sci.
49, 4377-4383
| Abstract »
| Full Text »
| PDF »
- Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell.
- B. Hein, K. I. Willig, and S. W. Hell (2008)
PNAS
105, 14271-14276
| Abstract »
| Full Text »
| PDF »
- Polarized microtubule arrays in apical dendrites and axons.
- A. C. Kwan, D. A. Dombeck, and W. W. Webb (2008)
PNAS
105, 11370-11375
| Abstract »
| Full Text »
| PDF »
- Fluorescence Changes of Genetic Calcium Indicators and OGB-1 Correlated with Neural Activity and Calcium In Vivo and In Vitro.
- T. Hendel, M. Mank, B. Schnell, O. Griesbeck, A. Borst, and D. F. Reiff (2008)
J. Neurosci.
28, 7399-7411
| Abstract »
| Full Text »
| PDF »
- Functional Stability of Retinal Ganglion Cells after Degeneration-Induced Changes in Synaptic Input.
- D. J. Margolis, G. Newkirk, T. Euler, and P. B. Detwiler (2008)
J. Neurosci.
28, 6526-6536
| Abstract »
| Full Text »
| PDF »
- In vivo two-photon voltage-sensitive dye imaging reveals top-down control of cortical layers 1 and 2 during wakefulness.
- B. Kuhn, W. Denk, and R. M. Bruno (2008)
PNAS
105, 7588-7593
| Abstract »
| Full Text »
| PDF »
- Dendritic Excitability and Synaptic Plasticity.
- P. J. Sjostrom, E. A. Rancz, A. Roth, and M. Hausser (2008)
Physiol Rev
88, 769-840
| Abstract »
| Full Text »
| PDF »
- Livin' on the Edge: Imaging Dendritic Spine Turnover in the Peri-Infarct Zone during Ischemic Stroke and Recovery.
- C. E. Brown and T. H. Murphy (2008)
Neuroscientist
14, 139-146
| Abstract »
| PDF »
- Nonlinear Integration of Binocular Optic Flow by DNOVS2, A Descending Neuron of the Fly.
- A. Wertz, A. Borst, and J. Haag (2008)
J. Neurosci.
28, 3131-3140
| Abstract »
| Full Text »
| PDF »
- Two-Photon Excitation of Potentiometric Probes Enables Optical Recording of Action Potentials From Mammalian Nerve Terminals In Situ.
- J. A. N. Fisher, J. R. Barchi, C. G. Welle, G.-H. Kim, P. Kosterin, A. L. Obaid, A. G. Yodh, D. Contreras, and B. M. Salzberg (2008)
J Neurophysiol
99, 1545-1553
| Abstract »
| Full Text »
| PDF »
- Single-molecule biophysics: at the interface of biology, physics and chemistry.
- A. A Deniz, S. Mukhopadhyay, and E. A Lemke (2008)
J R Soc Interface
5, 15-45
| Abstract »
| Full Text »
| PDF »
- The Application of Fluorescent Quantum Dots to Confocal, Multiphoton, and Electron Microscopic Imaging.
- T. J. Deerinck (2008)
Toxicol Pathol
36, 112-116
| Abstract »
| Full Text »
| PDF »
- Long-Term, High-Resolution Imaging in the Neocortex In Vivo.
- B. E. Chen, J. T. Trachtenberg, A. J.G.D. Holtmaat, and K. Svoboda (2008)
CSH Protocols
2008, pdb.prot4902
| Abstract »
| Full Text »
- In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia.
- M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam (2007)
PNAS
104, 19494-19499
| Abstract »
| Full Text »
| PDF »
- In Vivo Calcium Imaging of Neural Network Function.
- W. Gobel and F. Helmchen (2007)
Physiology
22, 358-365
| Abstract »
| Full Text »
| PDF »
- New Angles on Neuronal Dendrites In Vivo.
- W. Gobel and F. Helmchen (2007)
J Neurophysiol
98, 3770-3779
| Abstract »
| Full Text »
| PDF »
- Serotonin Modulates Dendritic Calcium Influx in Commissural Interneurons in the Mouse Spinal Locomotor Network.
- M. Diaz-Rios, D. A. Dombeck, W. W. Webb, and R. M. Harris-Warrick (2007)
J Neurophysiol
98, 2157-2167
| Abstract »
| Full Text »
| PDF »
- Principles of Multiphoton-Excitation Fluorescence Microscopy.
- W. Denk (2007)
CSH Protocols
2007, pdb.top23
| Abstract »
| Full Text »
- Rat liver sinusoidal endothelial cells survive without exogenous VEGF in 3D perfused co-cultures with hepatocytes.
- A. J. Hwa, R. C. Fry, A. Sivaraman, P. T. So, L. D. Samson, D. B. Stolz, and L. G. Griffith (2007)
FASEB J
21, 2564-2579
| Abstract »
| Full Text »
| PDF »
- Protracted Synaptogenesis after Activity-Dependent Spinogenesis in Hippocampal Neurons.
- U. V. Nagerl, G. Kostinger, J. C. Anderson, K. A. C. Martin, and T. Bonhoeffer (2007)
J. Neurosci.
27, 8149-8156
| Abstract »
| Full Text »
| PDF »
- Long-Term Two-Photon Transcranial Imaging of Synaptic Structures in the Living Brain.
- J. Grutzendler and W.-B. Gan (2007)
CSH Protocols
2007, pdb.prot4766
| Abstract »
| Full Text »
- Requirements for T Lymphocyte Migration in Explanted Lymph Nodes.
- J. H. Huang, L. I. Cardenas-Navia, C. C. Caldwell, T. J. Plumb, C. G. Radu, P. N. Rocha, T. Wilder, J. S. Bromberg, B. N. Cronstein, M. Sitkovsky, et al. (2007)
J. Immunol.
178, 7747-7755
| Abstract »
| Full Text »
| PDF »
- The Relationship between Blood Flow and Neuronal Activity in the Rodent Olfactory Bulb.
- E. Chaigneau, P. Tiret, J. Lecoq, M. Ducros, T. Knopfel, and S. Charpak (2007)
J. Neurosci.
27, 6452-6460
| Abstract »
| Full Text »
| PDF »
- Robust coding of flow-field parameters by axo-axonal gap junctions between fly visual interneurons.
- H. Cuntz, J. Haag, F. Forstner, I. Segev, and A. Borst (2007)
PNAS
104, 10229-10233
| Abstract »
| Full Text »
| PDF »
- Live imaging of collagen remodeling during angiogenesis.
- N. D. Kirkpatrick, S. Andreou, J. B. Hoying, and U. Utzinger (2007)
Am J Physiol Heart Circ Physiol
292, H3198-H3206
| Abstract »
| Full Text »
| PDF »
- Different Mechanisms Generate Maintained Activity in ON and OFF Retinal Ganglion Cells.
- D. J. Margolis and P. B. Detwiler (2007)
J. Neurosci.
27, 5994-6005
| Abstract »
| Full Text »
| PDF »
- Far-Field Optical Nanoscopy.
- S. W. Hell (2007)
Science
316, 1153-1158
| Abstract »
| Full Text »
| PDF »
- Two-Photon Calcium Imaging of Network Activity in XFP-Expressing Neurons in the Mouse.
- J. M. Wilson, D. A. Dombeck, M. Diaz-Rios, R. M. Harris-Warrick, and R. M. Brownstone (2007)
J Neurophysiol
97, 3118-3125
| Abstract »
| Full Text »
| PDF »
- Integration of Lobula Plate Output Signals by DNOVS1, an Identified Premotor Descending Neuron.
- J. Haag, A. Wertz, and A. Borst (2007)
J. Neurosci.
27, 1992-2000
| Abstract »
| Full Text »
| PDF »
- Hormonal Regulation of Nuclear Permeability.
- E. M. O'Brien, D. A. Gomes, S. Sehgal, and M. H. Nathanson (2007)
J. Biol. Chem.
282, 4210-4217
| Abstract »
| Full Text »
| PDF »
- Optical Recording of Action Potentials and Other Discrete Physiological Events: A Perspective from Signal Detection Theory.
- L. Sjulson and G. Miesenbock (2007)
Physiology
22, 47-55
| Abstract »
| Full Text »
| PDF »
- Multiphoton Fluorescence and Second Harmonic Generation Imaging of the Structural Alterations in Keratoconus Ex Vivo.
- H.-Y. Tan, Y. Sun, W. Lo, S.-J. Lin, C.-H. Hsiao, Y.-F. Chen, S. C.-M. Huang, W.-C. Lin, S.-H. Jee, H.-S. Yu, et al. (2006)
Invest. Ophthalmol. Vis. Sci.
47, 5251-5259
| Abstract »
| Full Text »
| PDF »
- Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing.
- M. Rueckel, J. A. Mack-Bucher, and W. Denk (2006)
PNAS
103, 17137-17142
| Abstract »
| Full Text »
| PDF »
- Spine Ca2+ Signaling in Spike-Timing-Dependent Plasticity.
- T. Nevian and B. Sakmann (2006)
J. Neurosci.
26, 11001-11013
| Abstract »
| Full Text »
| PDF »
- Exploring Mitochondria in the Intact Ischemic Heart: Advancing Technologies to Image Intracellular Function.
- M. N. Sack (2006)
Circulation
114, 1452-1454
| Full Text »
| PDF »
- Real-Time 2-Photon Imaging of Mitochondrial Function in Perfused Rat Hearts Subjected to Ischemia/Reperfusion.
- M. Matsumoto-Ida, M. Akao, T. Takeda, M. Kato, and T. Kita (2006)
Circulation
114, 1497-1503
| Abstract »
| Full Text »
| PDF »
- Aqueous humor outflow: what do we know? Where will it lead us?.
- M. P. Fautsch, D. H. Johnson, and the Second ARVO/Pfizer Research Institute Working (2006)
Invest. Ophthalmol. Vis. Sci.
47, 4181-4187
| Full Text »
| PDF »
- Two-Photon-Excited Fluorescence Imaging of Human RPE Cells with a Femtosecond Ti:Sapphire Laser..
- A. Bindewald-Wittich, M. Han, S. Schmitz-Valckenberg, S. R. Snyder, G. Giese, J. F. Bille, and F. G. Holz (2006)
Invest. Ophthalmol. Vis. Sci.
47, 4553-4557
| Abstract »
| Full Text »
| PDF »
- Nuclear Localization Signal Receptor Affinity Correlates with in Vivo Localization in Saccharomyces cerevisiae.
- A. E. Hodel, M. T. Harreman, K. F. Pulliam, M. E. Harben, J. S. Holmes, M. R. Hodel, K. M. Berland, and A. H. Corbett (2006)
J. Biol. Chem.
281, 23545-23556
| Abstract »
| Full Text »
| PDF »
- Macromolecular-scale resolution in biological fluorescence microscopy.
- G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Luhrmann, R. Jahn, C. Eggeling, and S. W. Hell (2006)
PNAS
103, 11440-11445
| Abstract »
| Full Text »
| PDF »
- Calcium Release Domains in Mammalian Skeletal Muscle Studied with Two-photon Imaging and Spot Detection Techniques.
- J. Gomez, P. Neco, M. DiFranco, and J. L. Vergara (2006)
J. Gen. Physiol.
127, 623-637
| Abstract »
| Full Text »
| PDF »
- Fanciful FRET.
- S. S. Vogel, C. Thaler, and S. V. Koushik (2006)
Sci. STKE
2006, re2
| Abstract »
| Full Text »
| PDF »
- Living cells as test tubes..
- X. S. Xie, J. Yu, and W. Y. Yang (2006)
Science
312, 228-230
| Abstract »
| Full Text »
| PDF »
- Approaches for biological and biomimetic energy conversion.
- D. A. LaVan and J. N. Cha (2006)
PNAS
103, 5251-5255
| Abstract »
| Full Text »
| PDF »
- Multiphoton autofluorescence and second-harmonic generation imaging of the ex vivo porcine eye..
- S.-W. Teng, H.-Y. Tan, J.-L. Peng, H.-H. Lin, K. H. Kim, W. Lo, Y. Sun, W.-C. Lin, S.-J. Lin, S.-H. Jee, et al. (2006)
Invest. Ophthalmol. Vis. Sci.
47, 1216-1224
| Abstract »
| Full Text »
| PDF »
- Overcoming photodamage in second-harmonic generation microscopy: Real-time optical recording of neuronal action potentials.
- L. Sacconi, D. A. Dombeck, and W. W. Webb (2006)
PNAS
103, 3124-3129
| Abstract »
| Full Text »
| PDF »
- Fast Functional Imaging of Single Neurons Using Random-Access Multiphoton (RAMP) Microscopy.
- V. Iyer, T. M. Hoogland, and P. Saggau (2006)
J Neurophysiol
95, 535-545
| Abstract »
| Full Text »
| PDF »
- Quantitative 3D fluorescence technique for the analysis of en face preparations of arterial walls using quantum dot nanocrystals and two-photon excitation laser scanning microscopy.
- D. E. Ferrara, D. Weiss, P. H. Carnell, R. P. Vito, D. Vega, X. Gao, S. Nie, and W. R. Taylor (2006)
Am J Physiol Regulatory Integrative Comp Physiol
290, R114-R123
| Abstract »
| Full Text »
| PDF »
- Diffusion and scaling during early embryonic pattern formation.
- T. Gregor, W. Bialek, R. R. d. R. van Steveninck, D. W. Tank, and E. F. Wieschaus (2005)
PNAS
102, 18403-18407
| Abstract »
| Full Text »
| PDF »
- Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.
- C. L. Evans, E. O. Potma, M. Puoris'haag, D. Cote, C. P. Lin, and X. S. Xie (2005)
PNAS
102, 16807-16812
| Abstract »
| Full Text »
| PDF »
- Temporally resolved interactions between antigen-stimulated IgE receptors and Lyn kinase on living cells.
- D. R. Larson, J. A. Gosse, D. A. Holowka, B. A. Baird, and W. W. Webb (2005)
J. Cell Biol.
171, 527-536
| Abstract »
| Full Text »
| PDF »
- Map Plasticity in Somatosensory Cortex.
- D. E. Feldman and M. Brecht (2005)
Science
310, 810-815
| Abstract »
| Full Text »
| PDF »
- Optical Recording of Fast Neuronal Membrane Potential Transients in Acute Mammalian Brain Slices by Second-Harmonic Generation Microscopy.
- D. A. Dombeck, L. Sacconi, M. Blanchard-Desce, and W. W. Webb (2005)
J Neurophysiol
94, 3628-3636
| Abstract »
| Full Text »
| PDF »
- A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets.
- H. Kasai, H. Hatakeyama, T. Kishimoto, T.-T. Liu, T. Nemoto, and N. Takahashi (2005)
J. Physiol.
568, 891-903
| Abstract »
| Full Text »
| PDF »
- Quantitative intravital microscopy using a Generalized Polarity concept for kidney studies.
- W. Yu, R. M. Sandoval, and B. A. Molitoris (2005)
Am J Physiol Cell Physiol
289, C1197-C1208
| Abstract »
| Full Text »
| PDF »
- Symposium Presentations.
- V. Fuster (2005)
J. Am. Coll. Cardiol.
46, 5A-70A
| Full Text »
| PDF »
- Imaging input and output dynamics of neocortical networks in vivo: Exciting times ahead.
- A. Grinvald (2005)
PNAS
102, 14125-14126
| Full Text »
| PDF »
- Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution.
- M. G. L. Gustafsson (2005)
PNAS
102, 13081-13086
| Abstract »
| Full Text »
| PDF »
- Donor Islet Endothelial Cells Participate in Formation of Functional Vessels Within Pancreatic Islet Grafts.
- D. Nyqvist, M. Kohler, H. Wahlstedt, and P.-O. Berggren (2005)
Diabetes
54, 2287-2293
| Abstract »
| Full Text »
| PDF »
- Two-Photon Excitation in Fluorescence Polarization Receptor-Ligand Binding Assay.
- M. E. Tirri, R. J. Huttunen, J. Toivonen, P. L. Harkonen, J. T. Soini, and P. E. Hanninen (2005)
J Biomol Screen
10, 314-319
| Abstract »
| PDF »
- Multiphoton imaging of renal tissues in vitro.
- J. Peti-Peterdi (2005)
Am J Physiol Renal Physiol
288, F1079-F1083
| Abstract »
| Full Text »
| PDF »
- In Vivo Performance of Genetically Encoded Indicators of Neural Activity in Flies.
- D. F. Reiff, A. Ihring, G. Guerrero, E. Y. Isacoff, M. Joesch, J. Nakai, and A. Borst (2005)
J. Neurosci.
25, 4766-4778
| Abstract »
| Full Text »
| PDF »
- Target Cell-Dependent Normalization of Transmitter Release at Neocortical Synapses.
- H. J. Koester and D. Johnston (2005)
Science
308, 863-866
| Abstract »
| Full Text »
| PDF »
- Sphingosine 1-phosphate induces cytoskeletal reorganization in C2C12 myoblasts: physiological relevance for stress fibres in the modulation of ion current through stretch-activated channels.
- L. Formigli, E. Meacci, C. Sassoli, F. Chellini, R. Giannini, F. Quercioli, B. Tiribilli, R. Squecco, P. Bruni, F. Francini, et al. (2005)
J. Cell Sci.
118, 1161-1171
| Abstract »
| Full Text »
| PDF »
- Multiphoton Microscopy of Endogenous Fluorescence Differentiates Normal, Precancerous, and Cancerous Squamous Epithelial Tissues.
- M. C. Skala, J. M. Squirrell, K. M. Vrotsos, J. C. Eickhoff, A. Gendron-Fitzpatrick, K. W. Eliceiri, and N. Ramanujam (2005)
Cancer Res.
65, 1180-1186
| Abstract »
| Full Text »
| PDF »
- Dynamic Multiphoton Imaging: A Live View from Cells to Systems.
- G. E. Stutzmann and I. Parker (2005)
Physiology
20, 15-21
| Abstract »
| Full Text »
| PDF »
- In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses.
- W. Supatto, D. Debarre, B. Moulia, E. Brouzes, J.-L. Martin, E. Farge, and E. Beaurepaire (2005)
PNAS
102, 1047-1052
| Abstract »
| Full Text »
| PDF »
- Two-Photon Microscopy of Cells and Tissue.
- M. Rubart (2004)
Circ. Res.
95, 1154-1166
| Abstract »
| Full Text »
| PDF »
- Use of coherent control methods through scattering biological tissue to achieve functional imaging.
- J. M. Dela Cruz, I. Pastirk, M. Comstock, V. V. Lozovoy, and M. Dantus (2004)
PNAS
101, 16996-17001
| Abstract »
| Full Text »
| PDF »
- A Genetic Switch for Epilepsy in Adult Mice.
- H. E. Krestel, D. R. Shimshek, V. Jensen, T. Nevian, J. Kim, Y. Geng, T. Bast, A. Depaulis, K. Schonig, F. Schwenk, et al. (2004)
J. Neurosci.
24, 10568-10578
| Abstract »
| Full Text »
| PDF »
- Guiding neuronal development with in situ microfabrication.
- B. Kaehr, R. Allen, D. J. Javier, J. Currie, and J. B. Shear (2004)
PNAS
101, 16104-16108
| Abstract »
| Full Text »
| PDF »
- Fly motion vision is based on Reichardt detectors regardless of the signal-to-noise ratio.
- J. Haag, W. Denk, and A. Borst (2004)
PNAS
101, 16333-16338
| Abstract »
| Full Text »
| PDF »
- Novel Approaches to Monitor and Manipulate Single Neurons In Vivo.
- M. Brecht, M. S. Fee, O. Garaschuk, F. Helmchen, T. W. Margrie, K. Svoboda, and P. Osten (2004)
J. Neurosci.
24, 9223-9227
| Full Text »
| PDF »
- Stabilization of Exocytosis by Dynamic F-actin Coating of Zymogen Granules in Pancreatic Acini.
- T. Nemoto, T. Kojima, A. Oshima, H. Bito, and H. Kasai (2004)
J. Biol. Chem.
279, 37544-37550
| Abstract »
| Full Text »
| PDF »
- Retinosomes: new insights into intracellular managing of hydrophobic substances in lipid bodies.
- Y. Imanishi, V. Gerke, and K. Palczewski (2004)
J. Cell Biol.
166, 447-453
| Abstract »
| Full Text »
| PDF »
- Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy.
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer (2004)
Science
305, 1007-1009
| Abstract »
| Full Text »
| PDF »
- Fluorescence imaging in living animals. Focus on "Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determined using intravital two-photon microscopy".
- S. X. Lin and F. R. Maxfield (2004)
Am J Physiol Cell Physiol
287, C257-C259
| Full Text »
| PDF »
- Neural Activity Triggers Neuronal Oxidative Metabolism Followed by Astrocytic Glycolysis.
- K. A. Kasischke, H. D. Vishwasrao, P. J. Fisher, W. R. Zipfel, and W. W. Webb (2004)
Science
305, 99-103
| Abstract »
| Full Text »
| PDF »
- Video-Rate Nonlinear Microscopy of Neuronal Membrane Dynamics With Genetically Encoded Probes.
- R. D. Roorda, T. M. Hohl, R. Toledo-Crow, and G. Miesenbock (2004)
J Neurophysiol
92, 609-621
| Abstract »
| Full Text »
| PDF »
- Acidosis-induced apoptosis in human and porcine heart.
- H. S. Thatte, J.-H. Rhee, S. E. Zagarins, P. R. Treanor, V. Birjiniuk, M. D. Crittenden, and S. F. Khuri (2004)
Ann. Thorac. Surg.
77, 1376-1383
| Abstract »
| Full Text »
| PDF »
- In Vivo Multiphoton Microscopy of Deep Brain Tissue.
- M. J. Levene, D. A. Dombeck, K. A. Kasischke, R. P. Molloy, and W. W. Webb (2004)
J Neurophysiol
91, 1908-1912
| Abstract »
| Full Text »
| PDF »
- The Number of Glutamate Receptors Opened by Synaptic Stimulation in Single Hippocampal Spines.
- E. A. Nimchinsky, R. Yasuda, T. G. Oertner, and K. Svoboda (2004)
J. Neurosci.
24, 2054-2064
| Abstract »
| Full Text »
| PDF »
- Single Spine Ca2+ Signals Evoked by Coincident EPSPs and Backpropagating Action Potentials in Spiny Stellate Cells of Layer 4 in the Juvenile Rat Somatosensory Barrel Cortex.
- T. Nevian and B. Sakmann (2004)
J. Neurosci.
24, 1689-1699
| Abstract »
| Full Text »
| PDF »
- Postsynaptic Calcium Influx at Single Synaptic Contacts between Pyramidal Neurons and Bitufted Interneurons in Layer 2/3 of Rat Neocortex Is Enhanced by Backpropagating Action Potentials.
- K. M. M. Kaiser, J. Lubke, Y. Zilberter, and B. Sakmann (2004)
J. Neurosci.
24, 1319-1329
| Abstract »
| Full Text »
| PDF »
- Imaging Calcium Concentration Dynamics in Small Neuronal Compartments.
- R. Yasuda, E. A. Nimchinsky, V. Scheuss, T. A. Pologruto, T. G. Oertner, B. L. Sabatini, and K. Svoboda (2004)
Sci. STKE
2004, pl5
| Abstract »
| Full Text »
| PDF »
- Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye.
- Y. Imanishi, M. L. Batten, D. W. Piston, W. Baehr, and K. Palczewski (2004)
J. Cell Biol.
164, 373-383
| Abstract »
| Full Text »
| PDF »
- Optical Recording of Action Potentials with Second-Harmonic Generation Microscopy.
- D. A. Dombeck, M. Blanchard-Desce, and W. W. Webb (2004)
J. Neurosci.
24, 999-1003
| Abstract »
| Full Text »
| PDF »
- Probing protein oligomerization in living cells with fluorescence fluctuation spectroscopy.
- Y. Chen, L.-N. Wei, and J. D. Muller (2003)
PNAS
100, 15492-15497
| Abstract »
| Full Text »
| PDF »
|
|