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Science 30 March 1990:
Vol. 247. no. 4950, pp. 1578 - 1581
DOI: 10.1126/science.2157284
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Articles

Science, Vol 247, Issue 4950, 1578-1581
Copyright © 1990 by American Association for the Advancement of Science

articles

Binding of GAP to activated PDGF receptors

A Kazlauskas, C Ellis, T Pawson, and JA Cooper

Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

The ras proto-oncogene products appear to relay intracellular signals via the Ras guanosine triphosphatase (GTPase) activator protein, GAP. In dog epithelial cells expressing human platelet-derived growth factor (PDGF) receptors, binding of PDGF caused approximately one-tenth of the total GAP molecules to complex with the receptor. Studies with mutant PDGF receptors showed that maximum association required both receptor kinase activity and phosphorylatable tyrosine residues at both the identified sites of receptor autophosphorylation.


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