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Science 9 March 1990:
Vol. 247. no. 4947, pp. 1208 - 1210
DOI: 10.1126/science.2156338
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Articles

Science, Vol 247, Issue 4947, 1208-1210
Copyright © 1990 by American Association for the Advancement of Science

articles

Steady-state coupling of ion-channel conformations to a transmembrane ion gradient

EA Richard and C Miller

Howard Hughes Medical Institute, Graduate Department of Biochemistry, Brandeis University, Waltham, MA 02254.

Under stationary conditions, opening and closing of single Torpedo electroplax chloride channels show that the number of transitions per unit time between inactivated and conducting states are unequal in opposite directions. This asymmetry, which increases with transmembrane electrochemical gradient for the chloride ion, violates the principle of microscopic reversibility and thus demonstrates that the channel-gating process is not at thermodynamic equilibrium. The results imply that the channel's conformational states are coupled to the transmembrane electrochemical gradient of the chloride ion.


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