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Science 2 February 1990:
Vol. 247. no. 4942, pp. 568 - 571
DOI: 10.1126/science.2300817
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Articles

Science, Vol 247, Issue 4942, 568-571
Copyright © 1990 by American Association for the Advancement of Science

articles

Tumorigenicity in human melanoma cell lines controlled by introduction of human chromosome 6

JM Trent, EJ Stanbridge, HL McBride, EU Meese, G Casey, DE Araujo, CM Witkowski, and RB Nagle

University of Arizona, Arizona Cancer Center, Tucson 85724.

Chromosome banding analysis of human malignant melanoma has documented the nonrandom alteration of chromosome 6. To determine the relevance of chromosome 6 abnormalities in melanoma, a normal chromosome 6 was directly introduced into melanoma cell lines. The resulting (+6) microcell hybrids were significantly altered in their phenotypic properties in culture and lost their ability to form tumors in nude mice. The loss of the chromosome 6 from melanoma microcell hybrids resulted in the reversion to tumorigenicity of these cells in mice. The introduction of the selectable marker (psv2neo) alone into melanoma cell lines had no effect on tumorigenicity. These results support the idea that one or more genes on chromosome 6 may control the malignant expression of human melanoma.


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