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Science 2 February 1990:
Vol. 247. no. 4942, pp. 546 - 552
DOI: 10.1126/science.1689074
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Articles

Science, Vol 247, Issue 4942, 546-552
Copyright © 1990 by American Association for the Advancement of Science

articles

Functional evidence for an RNA template in telomerase

D Shippen-Lentz and EH Blackburn

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

The RNA moiety of the ribonucleoprotein enzyme telomerase from the ciliate Euplotes crassus was identified and its gene was sequenced. Functional analysis, in which oligonucleotides complementary to portions of the telomerase RNA were tested for their ability to prime telomerase in vitro, showed that the sequence 5' CAAAACCCCAAA 3' in this RNA is the template for synthesis of telomeric TTTTGGGG repeats by the Euplotes telomerase. The data provide a direct demonstration of a template function for a telomerase RNA and demarcate the outer boundaries of the telomeric template. Telomerase can now be defined as a specialized reverse transcriptase.


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Telomerase Is Controlled by Protein Kinase Calpha in Human Breast Cancer Cells.
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Specific telomerase RNA residues distant from the template are essential for telomerase function.
J. Roy, T. B. Fulton, and E. H. Blackburn (1998)
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Human telomerase contains evolutionarily conserved catalytic and structural subunits.
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Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity.
J. Lingner, T. R. Cech, T. R. Hughes, and V. Lundblad (1997)
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An unusual histone H3 specific for early macronuclear development in Euplotes crassus.
C. L. Jahn, Z. Ling, C. M. Tebeau, and L. A. Klobutcher (1997)
PNAS 94, 1332-1337
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Est1 has the properties of a single-stranded telomere end-binding protein..
V Virta-Pearlman, D K Morris, and V Lundblad (1996)
Genes & Dev. 10, 3094-3104
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Cap-prevented recombination between terminal telomeric repeat arrays (telomere CPR) maintains telomeres in Kluyveromyces lactis lacking telomerase..
M J McEachern and E H Blackburn (1996)
Genes & Dev. 10, 1822-1834
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Telomeres: Beginning to Understand the End.
V. A. Zakian (1995)
Science 270, 1601-1607
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ATP-dependent Processivity of a Telomerase Activity from Saccharomyces cerevisiae.
N. F. Lue and J. C. Wang (1995)
J. Biol. Chem. 270, 21453-21456
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Altering specific telomerase RNA template residues affects active site function..
D Gilley, M S Lee, and E H Blackburn (1995)
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Boundary elements of the Tetrahymena telomerase RNA template and alignment domains..
C Autexier and C W Greider (1995)
Genes & Dev. 9, 2227-2239
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The RNA component of human telomerase.
J Feng, W. Funk, S. Wang, S. Weinrich, A. Avilion, C. Chiu, R. Adams, E Chang, R. Allsopp, J Yu, et al. (1995)
Science 269, 1236-1241
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Functional characterization and developmental regulation of mouse telomerase RNA.
M. Blasco, W Funk, B Villeponteau, and C. Greider (1995)
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Telomerase in yeast.
M Cohn and E. Blackburn (1995)
Science 269, 396-400
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Gel Shift and UV Cross-linking Analysis of Tetrahymena Telomerase.
L. Harrington, C. Hull, J. Crittenden, and C. Greider (1995)
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Chromosome end games.
T. Cech (1994)
Science 266, 387-388
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Telomerase RNAs of different ciliates have a common secondary structure and a permuted template..
J Lingner, L L Hendrick, and T R Cech (1994)
Genes & Dev. 8, 1984-1998
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Functional reconstitution of wild-type and mutant Tetrahymena telomerase..
C Autexier and C W Greider (1994)
Genes & Dev. 8, 563-575
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Oxytricha telomere-binding protein: separable DNA-binding and dimerization domains of the alpha-subunit..
G Fang, J T Gray, and T R Cech (1993)
Genes & Dev. 7, 870-882
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In Vivo and In Vitro Studies of Telomeres and Telomerase.
M.S. Lee, R.C. Gallagher, J. Bradley, and E.H. Blackburn (1993)
Cold Spring Harb Symp Quant Biol 58, 707-718
   Abstract »    PDF »
Telomerase and Telomere-length Regulation: Lessons from Small Eukaryotes to Mammals.
C.W. Greider (1993)
Cold Spring Harb Symp Quant Biol 58, 719-723
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Pseudo-templated transcription in prokaryotic and eukaryotic organisms..
J P Jacques and D Kolakofsky (1991)
Genes & Dev. 5, 707-713
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Replicative senescence: the human fibroblast comes of age.
S Goldstein (1990)
Science 249, 1129-1133
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Telomeres and their synthesis.
E. Blackburn (1990)
Science 249, 489-490
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Tetrahymena proteins p80 and p95 are not core telomerase components.
D. X. Mason, C. Autexier, and C. W. Greider (2001)
PNAS 98, 12368-12373
   Abstract »    Full Text »    PDF »


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