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Science 26 January 1990:
Vol. 247. no. 4941, pp. 449 - 450
DOI: 10.1126/science.247.4941.449
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Articles

A Regulatory Gene as a Novel Visible Marker for Maize Transformation

Steven R. Ludwig 1, Ben Bowen 2, Larry Beach 2, and Susan R. Wessler 1

1 Botany Department, University of Georgia, Athens, GA 30602
2 Pioneer Hi-Bred Intenational, Departmnent of Biotechnology Research, Johnston, IA 50131

The temporal and spatial patterns of anthocyanin pigmentation in the maize plant are determined by the presence or absence of the R protein product, a presumed transcriptional activator. At least 50 unique patterns of pigmentation, conditioned by members of the R gene family, have been described. In this study, microprojectiles were used to introduce into maize cells a vector containing the transcription unit from one of these genes (Lc) fused to a constitutive promoter. This chimeric gene induces cell autonomous pigmentation in tissues that are not normally pigmented by the Lc gene. As a reporter for gene expression studies in maize, R is unique because it can be quantified in living tissue simply by counting the number of pigmented cells following bombardment. R may also be useful as a visible marker for selecting stably transformed cell lineages that can give rise to transgenic plants.


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A. Lloyd, V Walbot, and R. Davis (1992)
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Allelic diversity of the maize B regulatory gene: different leader and promoter sequences of two B alleles determine distinct tissue specificities of anthocyanin production..
J P Radicella, D Brown, L A Tolar, and V L Chandler (1992)
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Functional analysis of the transcriptional activator encoded by the maize B gene: evidence for a direct functional interaction between two classes of regulatory proteins..
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Identification of functional domains in the maize transcriptional activator C1: comparison of wild-type and dominant inhibitor proteins..
S A Goff, K C Cone, and M E Fromm (1991)
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