Related Content
Search Google Scholar for:
|
|
Science 19 January 1990:
Vol. 247. no. 4940, pp. 320 - 322
DOI: 10.1126/science.2296721
|
|
Articles
Science, Vol 247, Issue 4940, 320-322
Copyright © 1990 by American Association for the Advancement of Science
Identification of geranylgeranyl-modified proteins in HeLa cells
CC Farnsworth,
MH Gelb,
and
JA Glomset
Howard Hughes Medical Institute Laboratory, University of Washington, Seattle 98195.
Previous studies have shown that animal cells contain isoprenoid-modified proteins and that one of these proteins, lamin B, contains a thioether-linked farnesyl group that is attached to cysteine. In the present study, a novel isoprenoid-modification was identified by labeling HeLa cells with [3H]mevalonic acid and analyzing proteolytic digests of the total cell protein. Radioactive fragments were purified from these digests and treated with Raney nickel. The released, labeled material was analyzed by gas-liquid chromatography (GC) and mass spectrometry (MS). This approach revealed that an all-trans geranylgeranyl group was a major isoprenoid modification.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Statins Cause Intracellular Accumulation of Amyloid Precursor Protein, {beta}-Secretase-cleaved Fragments, and Amyloid {beta}-Peptide via an Isoprenoid-dependent Mechanism.
- S. L. Cole, A. Grudzien, I. O. Manhart, B. L. Kelly, H. Oakley, and R. Vassar (2005)
J. Biol. Chem.
280, 18755-18770
| Abstract »
| Full Text »
| PDF »
- Lysine {beta}311 of Protein Geranylgeranyltransferase Type I Partially Replaces Magnesium.
- H. L. Hartman, K. E. Bowers, and C. A. Fierke (2004)
J. Biol. Chem.
279, 30546-30553
| Abstract »
| Full Text »
| PDF »
- Prenylation inhibitors: a novel class of antiviral agents.
- S. Einav and J. S. Glenn (2003)
J. Antimicrob. Chemother.
52, 883-886
| Abstract »
| Full Text »
| PDF »
- The Branch Point Enzyme of the Mevalonate Pathway for Protein Prenylation Is Overexpressed in the ob/ob Mouse and Induced by Adipogenesis.
- D. Vicent, E. Maratos-Flier, and C. R. Kahn (2000)
Mol. Cell. Biol.
20, 2158-2166
| Abstract »
| Full Text »
- Human Geranylgeranyl Diphosphate Synthase. cDNA CLONING AND EXPRESSION.
- T. Kuzuguchi, Y. Morita, I. Sagami, H. Sagami, and K. Ogura (1999)
J. Biol. Chem.
274, 5888-5894
| Abstract »
| Full Text »
| PDF »
- Protein Farnesyltransferase from Trypanosoma brucei. A HETERODIMER OF 61- AND 65-kDa SUBUNITS AS A NEW TARGET FOR ANTIPARASITE THERAPEUTICS.
- K. Yokoyama, P. Trobridge, F. S. Buckner, W. C. Van Voorhis, K. D. Stuart, and M. H. Gelb (1998)
J. Biol. Chem.
273, 26497-26505
| Abstract »
| Full Text »
| PDF »
- Inhibiting geranylgeranylation blocks growth and promotes apoptosis in pulmonary vascular smooth muscle cells.
- W. W. Stark Jr., M. A. Blaskovich, B. A. Johnson, Y. Qian, A. Vasudevan, B. Pitt, A. D. Hamilton, S. M. Sebti, and P. Davies (1998)
Am J Physiol Lung Cell Mol Physiol
275, L55-L63
| Abstract »
| Full Text »
| PDF »
- Isolation and Characterization of a Prenylcysteine Lyase from Bovine Brain.
- L. Zhang, W. R. Tschantz, and P. J. Casey (1997)
J. Biol. Chem.
272, 23354-23359
| Abstract »
| Full Text »
| PDF »
- .
- M. Raiteri, L. Arnaboldi, P. Mcgeady, M. H. Gelb, D. Verri, C. Tagliabue, P. Quarato, P. Ferraboschi, E. Santaniello, R. Paoletti, et al. (1997)
J. Pharmacol. Exp. Ther.
281, 1144-1153
| Abstract »
| Full Text »
- Direct Demonstration of Geranylgeranylation and Farnesylation of Ki-Ras in Vivo.
- C. A. Rowell, J. J. Kowalczyk, M. D. Lewis, and A. M. Garcia (1997)
J. Biol. Chem.
272, 14093-14097
| Abstract »
| Full Text »
| PDF »
- Isoprenylation of Plant Proteins in Vivo.
- C. A. Shipton, I. Parmryd, E. Swiezewska, B. Andersson, and G. Dallner (1995)
J. Biol. Chem.
270, 566-572
| Abstract »
| Full Text »
| PDF »
- Selective inhibition of ras-dependent transformation by a farnesyltransferase inhibitor.
- N. Kohl, S. Mosser, S. deSolms, E. Giuliani, D. Pompliano, S. Graham, R. Smith, E. Scolnick, A Oliff, and J. Gibbs (1993)
Science
260, 1934-1937
| Abstract »
| PDF »
- Benzodiazepine peptidomimetics: potent inhibitors of Ras farnesylation in animal cells.
- G. James, J. Goldstein, M. Brown, T. Rawson, T. Somers, R. McDowell, C. Crowley, B. Lucas, A. Levinson, and J. Marsters Jr (1993)
Science
260, 1937-1942
| Abstract »
| PDF »
- TcRho1, a Farnesylated Rho Family Homologue from Trypanosoma cruzi. CLONING, TRANS-SPLICING, AND PRENYLATION STUDIES.
- J. L. Nepomuceno-Silva, K. Yokoyama, L. D. B. de Mello, S. M. Mendonca, J. C. Paixao, R. Baron, J.-C. Faye, F. S. Buckner, W. C. Van Voorhis, M. H. Gelb, et al. (2001)
J. Biol. Chem.
276, 29711-29718
| Abstract »
| Full Text »
| PDF »
|
|
