Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 12 January 1990:
Vol. 247. no. 4939, pp. 212 - 214
DOI: 10.1126/science.1688472
Prev | Table of Contents | Next

Articles

Science, Vol 247, Issue 4939, 212-214
Copyright © 1990 by American Association for the Advancement of Science

articles

Localization of an acetylcholine receptor intron to the nuclear membrane

SA Berman, S Bursztajn, B Bowen, and W Gilbert

Department of Neuro-oncology, M. D. Anderson Hospital, Houston, TX 77030.

The first intron of the RNA for the acetylcholine receptor (AChR) alpha subunit shows a ringlike distribution around nuclei in multinucleated myotubes by in situ hybridization. This pattern is not observed for an actin intron or U1 RNA. Quantitation of the intron sequences reveals large variations in the amount of both the AChR and actin introns between nuclei within the same myotube, although all nuclei express equivalent amounts of U1 RNA. This differential RNA expression indicates that nuclei can individually control expression of messenger RNAs. The restricted distribution of the AChR intron RNA suggests a previously unknown step in RNA processing.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Activation and Cellular Localization of the Cyclosporine A-sensitive Transcription Factor NF-AT in Skeletal Muscle Cells.
K. L. Abbott, B. B. Friday, D. Thaloor, T.J. Murphy, and G. K. Pavlath (1998)
Mol. Biol. Cell 9, 2905-2916
   Abstract »    Full Text »
Higher level organization of individual gene transcription and RNA splicing.
Y Xing, C. Johnson, P. Dobner, and J. Lawrence (1993)
Science 259, 1326-1330
   Abstract »    PDF »
A three-dimensional view of precursor messenger RNA metabolism within the mammalian nucleus.
K. Carter, D Bowman, W Carrington, K Fogarty, J. McNeil, F. Fay, and J. Lawrence (1993)
Science 259, 1330-1335
   Abstract »    PDF »
Methodologies for specific intron and exon RNA localization in cultured cells by haptenized and fluorochromized probes.
R. Dirks, F. van de Rijke, S Fujishita, M van der Ploeg, and A. Raap (1993)
J. Cell Sci. 104, 1187-1197
   Abstract »    PDF »
Role of Chromosome Territories in the Functional Compartmentalization of the Cell Nucleus.
T. Cremer, A. Kurz, R. Zirbel, S. Dietzel, B. Rinke, E. Schrock, M.R. Speicher, U. Mathieu, A. Jauch, P. Emmerich, et al. (1993)
Cold Spring Harb Symp Quant Biol 58, 777-792
   Abstract »    PDF »
Mechanisms of Intracellular Transcript Localization and Export in Early Drosophila Embryos.
I. Davis, H. Francis-Lang, and D. Ish-Horowicz (1993)
Cold Spring Harb Symp Quant Biol 58, 793-798
   Abstract »    PDF »
Messenger RNA transport and HIV rev regulation.
D. Chang and P. Sharp (1990)
Science 249, 614-615
   PDF »
Compartmentalization of Acetylcholine Receptor Gene Expression during Development of the Neuromuscular Junction.
J.P. Changeux, C. Babinet, J.L. Bessereau, A. Bessis, A. Cartaud, J. Cartaud, P. Daubas, A. Devillers-Thiery, A. Duclert, J.A. Hill, et al. (1990)
Cold Spring Harb Symp Quant Biol 55, 381-396
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)