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Science 5 January 1990:
Vol. 247. no. 4938, pp. 69 - 72
DOI: 10.1126/science.2136774
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Articles

Science, Vol 247, Issue 4938, 69-72
Copyright © 1990 by American Association for the Advancement of Science

articles

U1-specific protein C needed for efficient complex formation of U1 snRNP with a 5' splice site

V Heinrichs, M Bach, G Winkelmann, and R Luhrmann

Institut fuer Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany.

One of the functions of U1 small nuclear ribonucleoprotein (snRNP) in the splicing reaction of pre-mRNA molecules is the recognition of the 5' splice site. U1 snRNP proteins as well as base-pair interactions between U1 snRNA and the 5' splice site are important for the formation of the snRNP-pre-mRNA complex. To determine which proteins are needed for complex formation, the ability of U1 snRNPs gradually depleted of the U1-specific proteins C, A, and 70k to bind to an RNA molecule containing a 5' splice site sequence was studied in a nitrocellulose filter binding assay. The most significant effect was always observed when protein C was removed, either alone or together with other U1-specific proteins; the binding was reduced by 50 to 60%. Complementation of protein C-deficient U1 snRNPs with purified C protein restored their 5' splice site binding activity. These data suggest that protein C may potentiate the base-pair interaction between U1 RNA and the 5' splice site.


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