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Science 22 December 1989:
Vol. 246. no. 4937, pp. 1595 - 1597
DOI: 10.1126/science.2688091
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Articles

Science, Vol 246, Issue 4937, 1595-1597
Copyright © 1989 by American Association for the Advancement of Science

articles

The anticodon contains a major element of the identity of arginine transfer RNAs

LH Schulman and H Pelka

Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, NY 10461.

The contribution of the anticodon to the discrimination between cognate and noncognate tRNAs by Escherichia coli Arg-tRNA synthetase has been investigated by in vitro synthesis and aminoacylation of elongator methionine tRNA (tRNA(mMet) mutants. Substitution of the Arg anticodon CCG for the Met anticodon CAU leads to a dramatic increase in Arg acceptance by tRNA(mMet). A nucleotide (A20) previously identified by others in the dihydrouridine loop of tRNA(Arg)s makes a smaller contribution to the conversion of tRNA(mMet) identity from Met to Arg. The combined anticodon and dihydrouridine loop mutations yield a tRNA(mMet) derivative that is aminoacylated with near-normal kinetics by the Arg-tRNA synthetase.


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