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Science 8 December 1989:
Vol. 246. no. 4935, pp. 1306 - 1309
DOI: 10.1126/science.2479986

Articles

Science, Vol 246, Issue 4935, 1306-1309
Copyright © 1989 by American Association for the Advancement of Science


articles

Vascular endothelial growth factor is a secreted angiogenic mitogen

DW Leung, G Cachianes, WJ Kuang, DV Goeddel, and N Ferrara

Department of Molecular Biology, Genetech, South San Francisco, CA 94080.

Vascular endothelial growth factor (VEGF) was purified from media conditioned by bovine pituitary folliculostellate cells (FC). VEGF is a heparin-binding growth factor specific for vascular endothelial cells that is able to induce angiogenesis in vivo. Complementary DNA clones for bovine and human VEGF were isolated from cDNA libraries prepared from FC and HL60 leukemia cells, respectively. These cDNAs encode hydrophilic proteins with sequences related to those of the A and B chains of platelet-derived growth factor. DNA sequencing suggests the existence of several molecular species of VEGF. VEGFs are secreted proteins, in contrast to other endothelial cell mitogens such as acidic or basic fibroblast growth factors and platelet-derived endothelial cell growth factor. Human 293 cells transfected with an expression vector containing a bovine or human VEGF cDNA insert secrete an endothelial cell mitogen that behaves like native VEGF.


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Target Practice: Lessons from Phase III Trials with Bevacizumab and Vatalanib in the Treatment of Advanced Colorectal Cancer.
M. Los, J. M. L. Roodhart, and E. E. Voest (2007)
Oncologist 12, 443-450
   Abstract »    Full Text »    PDF »
Vascular Endothelial Growth Factors: Biology and Current Status of Clinical Applications in Cardiovascular Medicine.
S. Yla-Herttuala, T. T. Rissanen, I. Vajanto, and J. Hartikainen (2007)
J. Am. Coll. Cardiol. 49, 1015-1026
   Abstract »    Full Text »    PDF »
Single-Chain Bifunctional Vascular Endothelial Growth Factor (VEGF)-Follicle-Stimulating Hormone (FSH)-C-Terminal Peptide (CTP) Is Superior to the Combination Therapy of Recombinant VEGF plus FSH-CTP in Stimulating Angiogenesis during Ovarian Folliculogenesis.
R. K. Trousdale, S. V. Pollak, J. Klein, L. Lobel, Y. Funahashi, N. Feirt, and J. W. Lustbader (2007)
Endocrinology 148, 1296-1305
   Abstract »    Full Text »    PDF »
Neuropilin-1 and neuropilin-2 enhance VEGF121 stimulated signal transduction by the VEGFR-2 receptor.
N. Shraga-Heled, O. Kessler, C. Prahst, J. Kroll, H. Augustin, and G. Neufeld (2007)
FASEB J 21, 915-926
   Abstract »    Full Text »    PDF »
Inhibition of heat shock protein 90 impairs epidermal growth factor-mediated signaling in gastric cancer cells and reduces tumor growth and vascularization in vivo.
S. A. Lang, D. Klein, C. Moser, A. Gaumann, G. Glockzin, M. H. Dahlke, W. Dietmaier, U. Bolder, H. J. Schlitt, E. K. Geissler, et al. (2007)
Mol. Cancer Ther. 6, 1123-1132
   Abstract »    Full Text »    PDF »
Biological Aspects and Binding Strategies of Vascular Endothelial Growth Factor in Renal Cell Carcinoma.
B. I. Rini and W.K. Rathmell (2007)
Clin. Cancer Res. 13, 741s-746s
   Abstract »    Full Text »    PDF »
Perspectives On: Local and Sustained Delivery of Angiogenic Growth Factors.
H. Lee, H. J. Chung, and T. G. Park (2007)
Journal of Bioactive and Compatible Polymers 22, 89-114
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Triamcinolone acetonide suppresses early proangiogenic response in retinal pigment epithelial cells after photodynamic therapy in vitro.
R Obata, A Iriyama, Y Inoue, H Takahashi, Y Tamaki, and Y Yanagi (2007)
Br J Ophthalmol 91, 100-104
   Abstract »    Full Text »    PDF »
Vascular endothelial growth factor biology: clinical implications for ocular treatments..
R B Bhisitkul (2006)
Br J Ophthalmol 90, 1542-1547
   Abstract »    Full Text »    PDF »
Low-Dose Dopamine Agonist Administration Blocks Vascular Endothelial Growth Factor (VEGF)-Mediated Vascular Hyperpermeability without Altering VEGF Receptor 2-Dependent Luteal Angiogenesis in a Rat Ovarian Hyperstimulation Model.
R. Gomez, M. Gonzalez-Izquierdo, R. C. Zimmermann, E. Novella-Maestre, I. Alonso-Muriel, J. Sanchez-Criado, J. Remohi, C. Simon, and A. Pellicer (2006)
Endocrinology 147, 5400-5411
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Requirement of Protein Kinase D Tyrosine Phosphorylation for VEGF-A165-induced Angiogenesis through Its Interaction and Regulation of Phospholipase C{gamma} Phosphorylation.
L. Qin, H. Zeng, and D. Zhao (2006)
J. Biol. Chem. 281, 32550-32558
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Secretion of Vascular Endothelial Growth Factor by Primary Human Fibroblasts at Senescence.
J.-P. Coppe, K. Kauser, J. Campisi, and C. M. Beausejour (2006)
J. Biol. Chem. 281, 29568-29574
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A Very Effective Treatment for Neovascular Macular Degeneration.
E. M. Stone (2006)
N. Engl. J. Med. 355, 1493-1495
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Antiangiogenic therapy in human gastrointestinal malignancies..
J Heidemann, D G Binion, W Domschke, and T Kucharzik (2006)
Gut 55, 1497-1511
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Recruitment of a Prostaglandin E Receptor Subtype, EP3-Expressing Bone Marrow Cells Is Crucial in Wound-Induced Angiogenesis.
E. Kamoshita, Y. Ikeda, M. Fujita, H. Amano, A. Oikawa, T. Suzuki, Y. Ogawa, S. Yamashina, S. Azuma, S. Narumiya, et al. (2006)
Am. J. Pathol. 169, 1458-1472
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