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Science 17 November 1989:
Vol. 246. no. 4932, pp. 911 - 916
DOI: 10.1126/science.2683088

Articles

Science, Vol 246, Issue 4932, 911-916
Copyright © 1989 by American Association for the Advancement of Science


articles

Scissors-grip model for DNA recognition by a family of leucine zipper proteins

CR Vinson, PB Sigler, and SL McKnight

Howard Hughes Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.

C/EBP is a sequence-specific DNA binding protein that regulates gene expression in certain mammalian cells. The region of the C/EBP polypeptide required for specific recognition of DNA is related in amino acid sequence to other regulatory proteins, including the Fos and Jun transforming proteins. It has been proposed that these proteins bind DNA via a bipartite structural motif, consisting of a dimerization interface termed the "leucine zipper" and a DNA contact surface termed the "basic region." An evaluation of the properties of conserved amino acids within the basic region of 11 deduced protein sequences, coupled with the observation that they are located at an invariant distance from the leucine zipper, has led to the formulation of a "scissors-grip" model for DNA binding. The architectural features of this model are well suited for interaction with directly abutted, dyadsymmetric DNA sequences. Data supportive of the model were obtained with chemical probes of protein: DNA complexes.


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Interactional Cloning of the 84-kDa Major Outer Dense Fiber Protein Odf84. LEUCINE ZIPPERS MEDIATE ASSOCIATIONS OF Odf84 AND Odf27.
X. Shao, H. A. Tarnasky, U. Schalles, R. Oko, and F. A. van der Hoorn (1997)
J. Biol. Chem. 272, 6105-6113
   Abstract »    Full Text »    PDF »
alpha -Helical Protein Assembly Motifs.
W. D. Kohn, C. T. Mant, and R. S. Hodges (1997)
J. Biol. Chem. 272, 2583-2586
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Identification of a novel vertebrate circadian clock-regulated gene encoding the protein nocturnin.
C. B. Green and J. C. Besharse (1996)
PNAS 93, 14884-14888
   Abstract »    Full Text »    PDF »
Regulation of CCAAT/Enhancer-binding Protein Family Members by Stimulation of Glutamate Receptors in Cultured Rat Cortical Astrocytes.
S. Yano, K. Fukunaga, M. Takiguchi, Y. Ushio, M. Mori, and E. Miyamoto (1996)
J. Biol. Chem. 271, 23520-23527
   Abstract »    Full Text »    PDF »
Constitutive Expression of the Gene for the Cell-specific p48 DNA-binding Subunit of Pancreas Transcription Factor 1in Cultured Cells Is under Control of Binding Sites for Transcription Factors Sp1 and alpha Cbf.
M. Knofler, A. Krapp, O. Hagenbuchle, and P. K. Wellauer (1996)
J. Biol. Chem. 271, 21993-22002
   Abstract »    Full Text »    PDF »
The c-Jun delta -Domain Inhibits Neuroendocrine Promoter Activity in a DNA Sequence- and Pituitary-specific Manner.
K. N. Farrow, N. Manning, F. Schaufele, and A. Gutierrez-Hartmann (1996)
J. Biol. Chem. 271, 17139-17146
   Abstract »    Full Text »    PDF »
Identification and Characterization of Basal and Cyclic AMP Response Elements in the Promoter of the Rat Hexokinase II Gene.
H. Osawa, R. B. Robey, R. L. Printz, and D. K. Granner (1996)
J. Biol. Chem. 271, 17296-17303
   Abstract »    Full Text »    PDF »
REVIEW {blacksquare} : Regulation of Gene Expression by Neural Signals.
S. E. Hyman (1996)
Neuroscientist 2, 217-224
   Abstract »    PDF »
The alpha-Isoform of the CCAAT/Enhancer-binding Protein Is Required for Mediating cAMP Responsiveness of the Phosphoenolpyruvate Carboxykinase Promoter in Hepatoma Cells.
W. J. Roesler, S. M. Crosson, C. Vinson, and P. J. McFie (1996)
J. Biol. Chem. 271, 8068-8074
   Abstract »    Full Text »    PDF »
CCAAT Box Enhancer Binding Protein alpha (C/EBP-alpha) Stimulates kappaB Element-mediated Transcription in Transfected Cells.
I. Vietor, I. C. Oliveira, and J. Vilcek (1996)
J. Biol. Chem. 271, 5595-5602
   Abstract »    Full Text »    PDF »
DNA Binding Specificity of the CCAAT/Enhancer-binding Protein Transcription Factor Family.
S. Osada, H. Yamamoto, T. Nishihara, and M. Imagawa (1996)
J. Biol. Chem. 271, 3891-3896
   Abstract »    Full Text »    PDF »
Design of a C/EBP-specific, Dominant-negative bZIP Protein with Both Inhibitory and Gain-of-function Properties.
M. Olive, S. C. Williams, C. Dezan, P. F. Johnson, and C. Vinson (1996)
J. Biol. Chem. 271, 2040-2047
   Abstract »    Full Text »    PDF »
Hypoxia-induced Protein Binding to O(2)-responsive Sequences on the Tyrosine Hydroxylase Gene.
M. L. Norris and D. E. Millhorn (1995)
J. Biol. Chem. 270, 23774-23779
   Abstract »    Full Text »    PDF »
The DNA Binding Activity of C/EBP Transcription Factors Is Regulated in the G[IMAGE] Phase of the Hepatocyte Cell Cycle.
B. Rana, Y. Xie, D. Mischoulon, N. L. R. Bucher, and S. R. Farmer (1995)
J. Biol. Chem. 270, 18123-18132
   Abstract »    Full Text »    PDF »
Concerted Participation of NF-kappaB and C/EBP Heteromer in Lipopolysaccharide Induction of Serum Amyloid A Gene Expression in Liver.
A. Ray and M. Hannink (1995)
J. Biol. Chem. 270, 7365-7374
   Abstract »    Full Text »    PDF »
Formation of a monomeric DNA binding domain by Skn-1 bZIP and homeodomain elements.
T. Blackwell, B Bowerman, Priess JR, and H Weintraub (1994)
Science 266, 621-628
   Abstract »    PDF »
RRN6 and RRN7 encode subunits of a multiprotein complex essential for the initiation of rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae..
D A Keys, L Vu, J S Steffan, J A Dodd, R T Yamamoto, Y Nogi, and M Nomura (1994)
Genes & Dev. 8, 2349-2362
   Abstract »    PDF »
Molecular and genetic characterization of GABP beta..
F C de la Brousse, E H Birkenmeier, D S King, L B Rowe, and S L McKnight (1994)
Genes & Dev. 8, 1853-1865
   Abstract »    PDF »



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