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Science 10 November 1989:
Vol. 246. no. 4931, pp. 803 - 806
DOI: 10.1126/science.2683083
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Articles

Science, Vol 246, Issue 4931, 803-806
Copyright © 1989 by American Association for the Advancement of Science

articles

Thymotaxin, a chemotactic protein, is identical to beta 2-microglobulin

C Dargemont, D Dunon, MA Deugnier, M Denoyelle, JM Girault, F Lederer, KH Le, F Godeau, JP Thiery, and BA Imhof

Laboratoire de Physiopathologie du Developpement CNRS, Paris, France.

Thymotaxin, an 11-kilodalton protein chemotactic for rat bone marrow hematopoietic precursors, was purified from media conditioned by a rat thymic epithelial cell line. The NH2-terminal sequence of thymotaxin was identical to that of rat beta 2-microglobulin (beta 2m). Antibodies to beta 2m removed thymotaxin activity from the fraction containing the 11-kilodalton protein. Chemotactic activity was observed with rat plasma beta 2m, human beta 2m, and mouse recombinant beta 2m, further supporting the identity of thymotaxin with beta 2m. The directional migration, as opposed to random movement, of the cells was also confirmed. The only rat bone marrow cells that migrated toward beta 2m were Thy1+ immature lymphoid cells devoid of T cell, B cell, and myeloid cell differentiation markers.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Gated Importation of Prothymocytes by Adult Mouse Thymus Is Coordinated with Their Periodic Mobilization from Bone Marrow.
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J. Immunol. 171, 3568-3575
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beta 2-Microglobulin Identified as an Apoptosis-Inducing Factor and Its Characterization.
M. Mori, Y. Terui, M. Ikeda, H. Tomizuka, M. Uwai, T. Kasahara, N. Kubota, T. Itoh, Y. Mishima, M. Douzono-Tanaka, et al. (1999)
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Synthesis and Turnover of beta 2-Microglobulin in Ad12-transformed Cells Defective in Assembly and Transport of Class I Major Histocompatibility Complex Molecules.
S. V. Mey-Tal, C. Schechter, and R. Ehrlich (1997)
J. Biol. Chem. 272, 353-361
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Enrichment and characterization of thymus-repopulating cells in stroma-dependent cultures of rat bone marrow.
Z Prakapas, M Denoyelle, C Dargemont, F. Kroese, J. Thiery, and M. Deugnier (1993)
J. Cell Sci. 104, 1039-1048
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Normal development of mice deficient in beta 2M, MHC class I proteins, and CD8+ T cells.
B. Koller, P Marrack, J. Kappler, and O Smithies (1990)
Science 248, 1227-1230
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Science. ISSN 0036-8075 (print), 1095-9203 (online)