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Science 10 November 1989:
Vol. 246. no. 4931, pp. 799 - 803
DOI: 10.1126/science.2554496
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Articles

Science, Vol 246, Issue 4931, 799-803
Copyright © 1989 by American Association for the Advancement of Science

articles

Germ-line transmission of a c-abl mutation produced by targeted gene disruption in ES cells

PL Schwartzberg, SP Goff, and EJ Robertson

Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians & Surgeons, New York, NY 10032.

A substitution mutation has been introduced into the c-abl locus of murine embryonic stem cells by homologous recombination between exogenously added DNA and the endogenous gene, and these cells have been used to generate chimeric mice. It is shown that the c-abl mutation was transmitted to progeny by several male chimeras. This work demonstrates the feasibility of germ-line transmission of a mutation introduced into a nonselectable autosomal gene by homologous recombination.


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