Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 3 November 1989:
Vol. 246. no. 4930, pp. 654 - 656
DOI: 10.1126/science.2814486
Prev | Table of Contents | Next

Articles

Science, Vol 246, Issue 4930, 654-656
Copyright © 1989 by American Association for the Advancement of Science

articles

Proton motive force involved in protein transport across the outer membrane of Aeromonas salmonicida

KR Wong and JT Buckley

Department of Biochemistry and Microbiology, University of Victoria, BC, Canada.

Many Gram-negative bacteria export proteins to the exterior. Some of these proteins are first secreted into the periplasm and then cross the outer membrane in a separate step. The source of energy required for the translocation is unknown. Export of the extracellular protein proaerolysin from the periplasm through the outer membrane of Aeromonas salmonicida is inhibited by a proton ionophore and by low extracellular pH. One possible explanation of these results is that a proton gradient across the outer membrane is required for export.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Involvement of the XpsN Protein in Formation of the XpsL-XpsM Complex in Xanthomonas campestris pv. campestris Type II Secretion Apparatus.
H.-M. Lee, S.-W. Tyan, W.-M. Leu, L.-Y. Chen, D. C. Chen, and N.-T. Hu (2001)
J. Bacteriol. 183, 528-535
   Abstract »    Full Text »
Structures of Gram-Negative Cell Walls and Their Derived Membrane Vesicles.
T. J. Beveridge (1999)
J. Bacteriol. 181, 4725-4733
   Full Text »
Structure-Function Analysis of XcpP, a Component Involved in General Secretory Pathway-Dependent Protein Secretion in Pseudomonas aeruginosa.
S. Bleves, M. Gérard-Vincent, A. Lazdunski, and A. Filloux (1999)
J. Bacteriol. 181, 4012-4019
   Abstract »    Full Text »
Nucleotide Triphosphates Are Required for the Transport of Glycolate Oxidase into Peroxisomes.
D. G. Brickner and L. J. Olsen (1998)
Plant Physiology 116, 309-317
   Abstract »    Full Text »
Studies on the Energetics of Proaerolysin Secretion across the Outer Membrane of Aeromonas Species. EVIDENCE FOR A REQUIREMENT FOR BOTH THE PROTONMOTIVE FORCE AND ATP.
L. Letellier, S. P. Howard, and J. T. Buckley (1997)
J. Biol. Chem. 272, 11109-11113
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)