Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 3 November 1989:
Vol. 246. no. 4930, pp. 641 - 646
DOI: 10.1126/science.2510296
Prev | Table of Contents | Next

Articles

Science, Vol 246, Issue 4930, 641-646
Copyright © 1989 by American Association for the Advancement of Science

articles

Mapping the Drosophila genome with yeast artificial chromosomes

D Garza, JW Ajioka, DT Burke, and DL Hartl

Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110-1095.

The ability to clone large fragments of DNA in yeast artificial chromosomes (YAC's) has created the possibility of obtaining global physical maps of complex genomes. For this application to be feasible, most sequences in complex genomes must be able to be cloned in YAC's, and most clones must be genetically stable and colinear with the genomic sequences from which they originated (that is, not liable to undergo rearrangement). These requirements have been met with a YAC library containing DNA fragments from Drosophila melanogaster ranging in size up to several hundred kilobase pairs. Preliminary characterization of the Drosophila YAC library was carried out by in situ hybridization of random clones and analysis of clones containing known sequences. The results suggest that most euchromatic sequences can be cloned. The library also contains clones in which the inserted DNA is derived from the centromeric heterochromatin. The locations of 58 clones collectively representing about 8 percent of the euchromatic genome are presented.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Profile of Daniel L. Hartl.
H. D. Tinsley (2007)
PNAS 104, 9111-9113
   Full Text »    PDF »
A BAC-Based Physical Map of the Major Autosomes of Drosophila melanogaster.
R. A. Hoskins, C. R. Nelson, B. P. Berman, T. R. Laverty, R. A. George, L. Ciesiolka, M. Naeemuddin, A. D. Arenson, J. Durbin, R. G. David, et al. (2000)
Science 287, 2271-2274
   Abstract »    Full Text »
Expression of the human CFTR gene from episomal oriP-EBNA1-YACs in mouse cells.
D. Huertas, S. Howe, A. McGuigan, and C. Huxley (2000)
Hum. Mol. Genet. 9, 617-629
   Abstract »    Full Text »    PDF »
Mapping the human Y chromosome by fingerprinting cosmid clones..
K Taylor, N Hornigold, D Conway, D Williams, Z Ulinowski, M Agochiya, P Fattorini, P de Jong, P F Little, and J Wolfe (1996)
Genome Res. 6, 235-248
   Abstract »    PDF »
Around the genomes: the Drosophila genome project..
G M Rubin (1996)
Genome Res. 6, 71-79
   PDF »
The Drosophila tissue polarity gene inturned acts cell autonomously and encodes a novel protein.
W. Park, J Liu, E. Sharp, and P. Adler (1996)
Development 122, 961-969
   Abstract »    PDF »
The human Y chromosome: overlapping DNA clones spanning the euchromatic region.
S Foote, D Vollrath, A Hilton, and D. Page (1992)
Science 258, 60-66
   Abstract »    PDF »
Sequence-tagged site (STS) content mapping of human chromosomes: theoretical considerations and early experiences..
E D Green and P Green (1991)
Genome Res. 1, 77-90
   Abstract »    PDF »
Toward cloning and mapping the genome of Drosophila.
J Merriam, M Ashburner, D. Hartl, and F. Kafatos (1991)
Science 254, 221-225
   Abstract »    PDF »
The Human Genome Project: Prospects and Implications for Clinical Medicine.
E. D. Green and R. H. Waterston (1991)
JAMA 266, 1966-1975
   Abstract »    PDF »
The E23 early gene of Drosophila encodes an ecdysone-inducible ATP-binding cassette transporter capable of repressing ecdysone-mediated gene activation.
T. Hock, T. Cottrill, J. Keegan, and D. Garza (2000)
PNAS 97, 9519-9524
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)