Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 27 October 1989:
Vol. 246. no. 4929, pp. 488 - 491
DOI: 10.1126/science.2683071
Prev | Table of Contents | Next

Articles

Science, Vol 246, Issue 4929, 488-491
Copyright © 1989 by American Association for the Advancement of Science

articles

Human chromosome 12 is required for elevated HIV-1 expression in human-hamster hybrid cells

CE Hart, CY Ou, JC Galphin, J Moore, LT Bacheler, JJ Wasmuth, SR Petteway Jr, and G Schochetman

Centers for Disease Control, Atlanta, GA 30333.

Host cell factors act together with regulatory genes of the human immunodeficiency virus (HIV) to control virus production. Human-Chinese hamster ovary hybrid cell clones were used to probe for human chromosomes involved in regulating HIV gene expression. DNA transfection experiments showed that 4 of 18 clones had high levels of HIV gene expression measured by both extracellular virus production and transactivation of the HIV long terminal repeat in the presence of the trans-activator (tat) gene. Karyotype analyses revealed a 94% concordance (17/18) between human chromosome 12 and HIV gene expression. Other chromosomes had an 11 to 72% concordance with virus production.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Nuclear Import of the Preintegration Complex Is Blocked upon Infection by Human Immunodeficiency Virus Type 1 in Mouse Cells.
N. Tsurutani, J. Yasuda, N. Yamamoto, B.-I. Choi, M. Kadoki, and Y. Iwakura (2007)
J. Virol. 81, 677-688
   Abstract »    Full Text »    PDF »
CD4-Specific Transgenic Expression of Human Cyclin T1 Markedly Increases Human Immunodeficiency Virus Type 1 (HIV-1) Production by CD4+ T Lymphocytes and Myeloid Cells in Mice Transgenic for a Provirus Encoding a Monocyte-Tropic HIV-1 Isolate.
J. Sun, T. Soos, V. N. KewalRamani, K. Osiecki, J. H. Zheng, L. Falkin, L. Santambrogio, D. R. Littman, and H. Goldstein (2006)
J. Virol. 80, 1850-1862
   Abstract »    Full Text »    PDF »
Hepatitis C Virus Subgenomic Replicons in the Human Embryonic Kidney 293 Cell Line.
S. Ali, C. Pellerin, D. Lamarre, and G. Kukolj (2004)
J. Virol. 78, 491-501
   Abstract »    Full Text »    PDF »
The Envelope Glycoprotein of Human Endogenous Retrovirus Type W Uses a Divergent Family of Amino Acid Transporters/Cell Surface Receptors.
D. Lavillette, M. Marin, A. Ruggieri, F. Mallet, F.-L. Cosset, and D. Kabat (2002)
J. Virol. 76, 6442-6452
   Abstract »    Full Text »    PDF »
Susceptibility of Rat-Derived Cells to Replication by Human Immunodeficiency Virus Type 1.
O. T. Keppler, W. Yonemoto, F. J. Welte, K. S. Patton, D. Iacovides, R. E. Atchison, T. Ngo, D. L. Hirschberg, R. F. Speck, and M. A. Goldsmith (2001)
J. Virol. 75, 8063-8073
   Abstract »    Full Text »    PDF »
Mouse-Human Heterokaryons Support Efficient Human Immunodeficiency Virus Type 1 Assembly.
R. Mariani, B. A. Rasala, G. Rutter, K. Wiegers, S. M. Brandt, H.-G. Krausslich, and N. R. Landau (2001)
J. Virol. 75, 3141-3151
   Abstract »    Full Text »    PDF »
Multiple Blocks to Human Immunodeficiency Virus Type 1 Replication in Rodent Cells.
P. D. Bieniasz and B. R. Cullen (2000)
J. Virol. 74, 9868-9877
   Abstract »    Full Text »    PDF »
A Block to Human Immunodeficiency Virus Type 1 Assembly in Murine Cells.
R. Mariani, G. Rutter, M. E. Harris, T. J. Hope, H.-G. Krausslich, and N. R. Landau (2000)
J. Virol. 74, 3859-3870
   Abstract »    Full Text »    PDF »
Human and Rodent Transcription Elongation Factor P-TEFb: Interactions with Human Immunodeficiency Virus Type 1 Tat and Carboxy-Terminal Domain Substrate.
Y. Ramanathan, S. M. Reza, T. M. Young, M. B. Mathews, and T. Pe'ery (1999)
J. Virol. 73, 5448-5458
   Abstract »    Full Text »    PDF »
Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin T.
K. Fujinaga, R. Taube, J. Wimmer, T. P. Cujec, and B. M. Peterlin (1999)
PNAS 96, 1285-1290
   Abstract »    Full Text »    PDF »
The interaction between HIV-1 Tat and human cyclin T1 requires zinc and a critical cysteine residue that is not conserved in the murine CycT1 protein.
M. E. Garber, P. Wei, V. N. KewalRamani, T. P. Mayall, C. H. Herrmann, A. P. Rice, D. R. Littman, and K. A. Jones (1998)
Genes & Dev. 12, 3512-3527
   Abstract »    Full Text »
Identification of Limiting Steps for Efficient Trans-activation of HIV-1 Promoter by Tat in Saccharomyces cerevisiae.
L. Daviet, F. Bois, P.-L. Battisti, and A. Gatignol (1998)
J. Biol. Chem. 273, 28219-28228
   Abstract »    Full Text »    PDF »
The Secondary Structure of the R Region of a Murine Leukemia Virus Is Important for Stimulation of Long Terminal Repeat-Driven Gene Expression.
L. Cupelli, S. A. Okenquist, A. Trubetskoy, and J. Lenz (1998)
J. Virol. 72, 7807-7814
   Abstract »    Full Text »    PDF »
Activation of Integrated Provirus Requires Histone Acetyltransferase. p300 AND P/CAF ARE COACTIVATORS FOR HIV-1 Tat.
M. Benkirane, R. F. Chun, H. Xiao, V. V. Ogryzko, B. H. Howard, Y. Nakatani, and K.-T. Jeang (1998)
J. Biol. Chem. 273, 24898-24905
   Abstract »    Full Text »    PDF »
Rabbit Cells Expressing Human CD4 and Human CCR5 Are Highly Permissive for Human Immunodeficiency Virus Type 1 Infection.
R. F. Speck, M. L. Penn, J. Wimmer, U. Esser, B. F. Hague, T. J. Kindt, R. E. Atchison, and M. A. Goldsmith (1998)
J. Virol. 72, 5728-5734
   Abstract »    Full Text »    PDF »
The HIV transactivator TAT binds to the CDK-activating kinase and activates the phosphorylation of the carboxy-terminal domain of RNA polymerase II.
T. P. Cujec, H. Okamoto, K. Fujinaga, J. Meyer, H. Chamberlin, D. O. Morgan, and B. M. Peterlin (1997)
Genes & Dev. 11, 2645-2657
   Abstract »    Full Text »    PDF »
CXCR4/fusin Is Not a Species-specific Barrier in Murine Cells for HIV-1 Entry.
K. Tachibana, T. Nakajima, A. Sato, K. Igarashi, H. Shida, H. Iizasa, N. Yoshida, O. Yoshie, T. Kishimoto, and T. Nagasawa (1997)
J. Exp. Med. 185, 1865-1870
   Abstract »    Full Text »    PDF »
Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR.
A Gatignol, A Buckler-White, B Berkhout, and K. Jeang (1991)
Science 251, 1597-1600
   Abstract »    PDF »
Efficient assembly of an HIV-1/MLV Gag-chimeric virus in murine cells.
B. K. Chen, I. Rousso, S. Shim, and P. S. Kim (2001)
PNAS 98, 15239-15244
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)