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Science 6 October 1989:
Vol. 246. no. 4926, pp. 121 - 124
DOI: 10.1126/science.2506643
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Articles

Science, Vol 246, Issue 4926, 121-124
Copyright © 1989 by American Association for the Advancement of Science

articles

Fibroblasts transformed with v-src show enhanced formation of an inositol tetrakisphosphate

RM Johnson, WJ Wasilenko, RR Mattingly, MJ Weber, and JC Garrison

Department of Pharmacology, University of Virginia School of Medicine, Charlottesville 22908.

The tyrosine kinase pp60v-src, encoded by the v-src oncogene, seems to regulate phosphatidylinositol metabolism. The effect of pp60v-src on control points in inositol phosphate production was examined by measuring the amounts of inositol polyphosphates in Rat-1 cells expressing wild-type or mutant forms of the protein. Expression of v-src-resulted in a five- to sevenfold elevation in the steady-state amount of an isomer of inositol tetrakisphosphate, whereas the concentrations of inositol trisphosphates or other inositol tetrakisphosphates were not affected. The activity of a key enzyme in the formation of inositol tetrakisphosphates, inositol (1,4,5)-trisphosphate 3-kinase, was increased six- to eightfold in cytosolic extracts prepared from the v-src-transformed cells, suggesting that this enzyme may be one target for the pp60v-src kinase and that it may participate in the synthesis of novel, higher order inositol phosphates.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Role for Rat Inositol Polyphosphate Kinases rIPK2 and rIPK1 in Inositol Pentakisphosphate and Inositol Hexakisphosphate Production in Rat-1 Cells.
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pp60[IMAGE] -mediated Phosphorylation of Connexin 43, a Gap Junction Protein.
L. W. M. Loo, J. M. Berestecky, M. Y. Kanemitsu, and A. F. Lau (1995)
J. Biol. Chem. 270, 12751-12761
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Molecular cloning and expression of a complementary DNA for inositol 1,4,5-trisphosphate 3-kinase.
K. Choi, H. Kim, S. Lee, K. Moon, S. Sim, J. Kim, H. Chung, and S. Rhee (1990)
Science 248, 64-66
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Novel Function of Phosphoinositide 3-Kinase in T Cell Ca2+ Signaling. A PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE-MEDIATED Ca2+ ENTRY MECHANISM.
A.-L. Hsu, T.-T. Ching, G. Sen, D.-S. Wang, S. Bondada, K. S. Authi, and C.-S. Chen (2000)
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Phosphoinositide 3-Kinase Facilitates Antigen-stimulated Ca2+ Influx in RBL-2H3 Mast Cells via a Phosphatidylinositol 3,4,5-Trisphosphate-sensitive Ca2+ Entry Mechanism.
T.-T. Ching, A.-L. Hsu, A. J. Johnson, and C.-S. Chen (2001)
J. Biol. Chem. 276, 14814-14820
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