Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 29 September 1989:
Vol. 245. no. 4925, pp. 1496 - 1499
DOI: 10.1126/science.2551043
Prev | Table of Contents | Next

Articles

Science, Vol 245, Issue 4925, 1496-1499
Copyright © 1989 by American Association for the Advancement of Science

articles

Transformation by v-sis occurs by an internal autoactivation mechanism

BE Bejcek, DY Li, and TF Deuel

Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.

Transformation by the v-sis oncogene appears to require an interaction of its protein product, p28v-sis, with the receptor for the platelet-derived growth factor (PDGF). However, this interaction may not occur at the cell surface as predicted by the autocrine hypothesis because phenotypic transformation was not reversed by incubation of SSV-NRK cells with antisera to PDGF and because morphological transformation did not occur when nontransformed NRK cells were cultured continuously with p28v-sis. A mutant of the wild-type v-sis gene was constructed that encodes a v-sis protein targeted for retention within the endoplasmic reticulum and Golgi. NRK cells expressing the mutant v-sis gene did not secrete any detectable v-sis protein but were as fully transformed as wild-type v-sis transfectants. The results support a mechanism of transformation by v-sis in which internal activation of the PDGF receptor occurs before expression of either p28v-sis or the PDGF receptor at the cell surface.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Ligand-independent Thrombopoietin Mutant Receptor Requires Cell Surface Localization for Endogenous Activity.
C. Marty, R. Chaligne, C. Lacout, S. N. Constantinescu, W. Vainchenker, and J.-L. Villeval (2009)
J. Biol. Chem. 284, 11781-11791
   Abstract »    Full Text »    PDF »
Neoplasia Driven by Mutant c-KIT Is Mediated by Intracellular, Not Plasma Membrane, Receptor Signaling.
Z. Xiang, F. Kreisel, J. Cain, A. Colson, and M. H. Tomasson (2007)
Mol. Cell. Biol. 27, 267-282
   Abstract »    Full Text »    PDF »
Precursor B Cell Receptor Signaling Activity Can Be Uncoupled from Surface Expression..
F. B. Guloglu and C. A. J. Roman (2006)
J. Immunol. 176, 6862-6872
   Abstract »    Full Text »    PDF »
Autocrine Platelet-Derived Growth Factor-Dependent Gene Expression in Glioblastoma Cells Is Mediated Largely by Activation of the Transcription Factor Sterol Regulatory Element Binding Protein and Is Associated with Altered Genotype and Patient Survival in Human Brain Tumors.
D. Ma, C. L. Nutt, P. Shanehsaz, X. Peng, D. N. Louis, and D. M. Kaetzel (2005)
Cancer Res. 65, 5523-5534
   Abstract »    Full Text »    PDF »
Tyrosine Phosphorylation Regulates Maturation of Receptor Tyrosine Kinases.
D.-E. Schmidt-Arras, A. Bohmer, B. Markova, C. Choudhary, H. Serve, and F.-D. Bohmer (2005)
Mol. Cell. Biol. 25, 3690-3703
   Abstract »    Full Text »    PDF »
Kaposi's Sarcoma-associated Herpesvirus-encoded Viral Interleukin-6 Is Secreted and Modified Differently Than Human Interleukin-6: EVIDENCE FOR A UNIQUE AUTOCRINE SIGNALING MECHANISM.
M. B. Meads and P. G. Medveczky (2004)
J. Biol. Chem. 279, 51793-51803
   Abstract »    Full Text »    PDF »
Cloning, Expression, Characterization, and Role in Autocrine Cell Growth of Cell Surface Retention Sequence Binding Protein-1.
S. S. Huang, F.-M. Tang, Y.-H. Huang, I-H. Liu, S.-C. Hsu, S.-T. Chen, and J. S. Huang (2003)
J. Biol. Chem. 278, 43855-43869
   Abstract »    Full Text »    PDF »
The role of IFN{gamma} nuclear localization sequence in intracellular function.
C. M. I. Ahmed, M. A. Burkhart, M. G. Mujtaba, P. S. Subramaniam, and H. M. Johnson (2003)
J. Cell Sci. 116, 3089-3098
   Abstract »    Full Text »    PDF »
Transforming Growth Factor-beta 1 Mutations in Camurati-Engelmann Disease Lead to Increased Signaling by Altering either Activation or Secretion of the Mutant Protein.
K. Janssens, P. ten Dijke, S. H. Ralston, C. Bergmann, and W. Van Hul (2003)
J. Biol. Chem. 278, 7718-7724
   Abstract »    Full Text »    PDF »
Disruption of Transforming Growth Factor {beta} Signaling by a Novel Ligand-dependent Mechanism.
T. Fernandez, S. Amoroso, S. Sharpe, G. M. Jones, V. Bliskovski, A. Kovalchuk, L. M. Wakefield, S.-J. Kim, M. Potter, and J. J. Letterio (2002)
J. Exp. Med. 195, 1247-1255
   Abstract »    Full Text »    PDF »
Mechanism of Action and In Vivo Role of Platelet-Derived Growth Factor.
C.-H. Heldin and B. Westermark (1999)
Physiol Rev 79, 1283-1316
   Abstract »    Full Text »    PDF »
Removal of the Membrane-anchoring Domain of Epidermal Growth Factor Leads to Intracrine Signaling and Disruption of Mammary Epithelial Cell Organization.
H. S. Wiley, M. F. Woolf, L. K. Opresko, P. M. Burke, B. Will, J. R. Morgan, and D. A. Lauffenburger (1998)
J. Cell Biol. 143, 1317-1328
   Abstract »    Full Text »    PDF »
Induction of Unresponsiveness to Tumor Necrosis Factor (TNF) after Autocrine TNF Expression Requires TNF Membrane Retention.
E. Decoster, B. Vanhaesebroeck, E. Boone, S. Plaisance, K. De Vos, G. Haegeman, J. Grooten, and W. Fiers (1998)
J. Biol. Chem. 273, 3271-3277
   Abstract »    Full Text »    PDF »
Integrins, Oncogenes, and Anchorage Independence.
M. A. Schwartz (1997)
J. Cell Biol. 139, 575-578
   Full Text »    PDF »
The N-terminal propiece of interleukin 1alpha is a transforming nuclear oncoprotein.
F. T. Stevenson, J. Turck, R. M. Locksley, and D. H. Lovett (1997)
PNAS 94, 508-513
   Abstract »    Full Text »    PDF »
c-sis/PDGF-B Promoter Transactivation by the Tax Protein of Human T-cell Leukemia Virus Type 1.
S. R. Trejo, W. E. Fahl, and L. Ratner (1996)
J. Biol. Chem. 271, 14584-14590
   Abstract »    Full Text »    PDF »
Transcriptional Regulation of the SIS/PDGF-B Gene in Human Osteosarcoma Cells by the Sp Family of Transcription Factors.
Y. Liang, D. F. Robinson, Jör. Dennig, G. Suske, and W. E. Fahl (1996)
J. Biol. Chem. 271, 11792-11797
   Abstract »    Full Text »    PDF »
Tumor sialomucin complexes as tumor antigens and modulators of cellular interactions and proliferation.
K. L. Carraway, N. Fregien, K. L. Carraway, and C. A. Carraway (1992)
J. Cell Sci. 103, 299-307
   PDF »
Ras mediates translation initiation factor 4E-induced malignant transformation..
A Lazaris-Karatzas, M R Smith, R M Frederickson, M L Jaramillo, Y L Liu, H F Kung, and N Sonenberg (1992)
Genes & Dev. 6, 1631-1642
   Abstract »    PDF »
Autocrine Secretion--10 Years Later.
M. B. Sporn and A. B. Roberts (1992)
Ann Intern Med 117, 408-414
   Abstract »    PDF »
Nuclear Import of Insulin-like Growth Factor-binding Protein-3 and -5 Is Mediated by the Importin beta Subunit.
L. J. Schedlich, S. L. Le Page, S. M. Firth, L. J. Briggs, D. A. Jans, and R. C. Baxter (2000)
J. Biol. Chem. 275, 23462-23470
   Abstract »    Full Text »    PDF »
Platelet-derived Growth Factor (PDGF) Receptor-alpha Activates c-Jun NH2-terminal Kinase-1 and Antagonizes PDGF Receptor-beta -induced Phenotypic Transformation.
J. Yu, T. F. Deuel, and H.-R. C. Kim (2000)
J. Biol. Chem. 275, 19076-19082
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)