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Science 22 September 1989:
Vol. 245. no. 4924, pp. 1396 - 1399
DOI: 10.1126/science.2476851
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Articles

Science, Vol 245, Issue 4924, 1396-1399
Copyright © 1989 by American Association for the Advancement of Science

articles

Sequence-specific isotope effects on the cleavage of DNA by bleomycin

JW Kozarich, L Worth Jr, BL Frank, DF Christner, DE Vanderwall, and J Stubbe

Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742.

Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4'-hydrogen. A DNA fragment has been prepared that contains [4'-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology.


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