Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 22 September 1989:
Vol. 245. no. 4924, pp. 1392 - 1394
DOI: 10.1126/science.2571188
Prev | Table of Contents | Next

Articles

Science, Vol 245, Issue 4924, 1392-1394
Copyright © 1989 by American Association for the Advancement of Science

articles

The protein kinase domain of the ANP receptor is required for signaling

M Chinkers and DL Garbers

Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232.

A plasma membrane form of guanylate cyclase is a cell surface receptor for atrial natriuretic peptide (ANP). In response to ANP binding, the receptor-enzyme produces increased amounts of the second messenger, guanosine 3',5'-monophosphate. Maximal activation of the cyclase requires the presence of adenosine 5'-triphosphate (ATP) or nonhydrolyzable ATP analogs. The intracellular region of the receptor contains at least two domains with homology to other proteins, one possessing sequence similarity to protein kinase catalytic domains, the other to regions of unknown function in a cytoplasmic form of guanylate cyclase and in adenylate cyclase. It is now shown that the protein kinase-like domain functions as a regulatory element and that the second domain possesses catalytic activity. When the kinase-like domain was removed by deletion mutagenesis, the resulting ANP receptor retained guanylate cyclase activity, but this activity was independent of ANP and its stimulation by ATP was markedly reduced. A model for signal transduction is suggested in which binding of ANP to the extracellular domain of its receptor initiates a conformational change in the protein kinase-like domain, resulting in derepression of guanylate cyclase activity.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Guanylyl cyclase-D in the olfactory CO2 neurons is activated by bicarbonate.
L. Sun, H. Wang, J. Hu, J. Han, H. Matsunami, and M. Luo (2009)
PNAS 106, 2041-2046
   Abstract »    Full Text »    PDF »
Adenine nucleotides decrease the apparent Km of endogenous natriuretic peptide receptors for GTP.
L. K. Antos and L. R. Potter (2007)
Am J Physiol Endocrinol Metab 293, E1756-E1763
   Abstract »    Full Text »    PDF »
Intact Kinase Homology Domain of Natriuretic Peptide Receptor-B Is Essential for Skeletal Development.
R. Hachiya, Y. Ohashi, Y. Kamei, T. Suganami, H. Mochizuki, N. Mitsui, M. Saitoh, M. Sakuragi, G. Nishimura, H. Ohashi, et al. (2007)
J. Clin. Endocrinol. Metab. 92, 4009-4014
   Abstract »    Full Text »    PDF »
Natriuretic Peptides, Their Receptors, and Cyclic Guanosine Monophosphate-Dependent Signaling Functions.
L. R. Potter, S. Abbey-Hosch, and D. M. Dickey (2006)
Endocr. Rev. 27, 47-72
   Abstract »    Full Text »    PDF »
Cardiac-specific attenuation of natriuretic peptide A receptor activity accentuates adverse cardiac remodeling and mortality in response to pressure overload.
J. B. Patel, M. L. Valencik, A. M. Pritchett, J. C. Burnett Jr., J. A. McDonald, and M. M. Redfield (2005)
Am J Physiol Heart Circ Physiol 289, H777-H784
   Abstract »    Full Text »    PDF »
Down-Regulation Does Not Mediate Natriuretic Peptide-Dependent Desensitization of Natriuretic Peptide Receptor (NPR)-A or NPR-B: Guanylyl Cyclase-Linked Natriuretic Peptide Receptors Do Not Internalize.
D. Fan, P. M. Bryan, L. K. Antos, R. J. Potthast, and L. R. Potter (2005)
Mol. Pharmacol. 67, 174-183
   Abstract »    Full Text »    PDF »
The Drosophila Receptor Guanylyl Cyclase Gyc76C Is Required for Semaphorin-1a-Plexin A-Mediated Axonal Repulsion.
J. C. Ayoob, H.-H. Yu, J. R. Terman, and A. L. Kolodkin (2004)
J. Neurosci. 24, 6639-6649
   Abstract »    Full Text »    PDF »
Forced Homodimerization by Site-Directed Mutagenesis Alters Guanylyl Cyclase Activity of Natriuretic Peptide Receptor B.
T. Langenickel, J. Buttgereit, I. Pagel, R. Dietz, R. Willenbrock, and M. Bader (2004)
Hypertension 43, 460-465
   Abstract »    Full Text »    PDF »
17{beta}-Estradiol Antagonizes Cardiomyocyte Hypertrophy by Autocrine/Paracrine Stimulation of a Guanylyl Cyclase A Receptor-Cyclic Guanosine Monophosphate-Dependent Protein Kinase Pathway.
F. A. Babiker, L. J. De Windt, M. van Eickels, V. Thijssen, R. J.P. Bronsaer, C. Grohe, M. van Bilsen, and P. A. Doevendans (2004)
Circulation 109, 269-276
   Abstract »    Full Text »    PDF »
Regulation of the Guanylyl Cyclase-B Receptor by Alternative Splicing.
N. Tamura and D. L. Garbers (2003)
J. Biol. Chem. 278, 48880-48889
   Abstract »    Full Text »    PDF »
Structure, Regulation, and Function of Mammalian Membrane Guanylyl Cyclase Receptors, With a Focus on Guanylyl Cyclase-A.
M. Kuhn (2003)
Circ. Res. 93, 700-709
   Abstract »    Full Text »    PDF »
Natriuretic Peptide Receptor A Activation Stabilizes a Membrane-distal Dimer Interface.
A. De Lean, N. McNicoll, and J. Labrecque (2003)
J. Biol. Chem. 278, 11159-11166
   Abstract »    Full Text »    PDF »
The Protein Kinase Complement of the Human Genome.
G. Manning, D. B. Whyte, R. Martinez, T. Hunter, and S. Sudarsanam (2002)
Science 298, 1912-1934
   Abstract »    Full Text »    PDF »
Atrial natriuretic peptide mimetics and vasopeptidase inhibitors.
G. A. Sagnella (2001)
Cardiovasc Res 51, 416-428
   Abstract »    Full Text »    PDF »
Water and sodium regulation in chronic heart failure: the role of natriuretic peptides and vasopressin.
P. R Kalra, S. D Anker, and A. J.S Coats (2001)
Cardiovasc Res 51, 495-509
   Full Text »    PDF »
The role of C-type natriuretic peptide in cardiovascular medicine.
P.R Kalra, S.D Anker, A.D Struthers, and A.J.S Coats (2001)
Eur. Heart J. 22, 997-1007
   PDF »
Guanylyl Cyclases and Signaling by Cyclic GMP.
K. A. Lucas, G. M. Pitari, S. Kazerounian, I. Ruiz-Stewart, J. Park, S. Schulz, K. P. Chepenik, and S. A. Waldman (2000)
Pharmacol. Rev. 52, 375-414
   Abstract »    Full Text »    PDF »
A Transmembrane Guanylyl Cyclase (DAF-11) and Hsp90 (DAF-21) Regulate a Common Set of Chemosensory Behaviors in Caenorhabditis elegans.
D. A. Birnby, E. M. Link, J. J. Vowels, H. Tian, P. L. Colacurcio, and J. H. Thomas (2000)
Genetics 155, 85-104
   Abstract »    Full Text »
Extracellular Signal-regulated Protein Kinase Activation Is Required for the Anti-hypertrophic Effect of Atrial Natriuretic Factor in Neonatal Rat Ventricular Myocytes.
M. Silberbach, T. Gorenc, R. E. Hershberger, P. J. S. Stork, P. S. Steyger, and C. T. Roberts Jr. (1999)
J. Biol. Chem. 274, 24858-24864
   Abstract »    Full Text »    PDF »
A Constitutively "Phosphorylated" Guanylyl Cyclase-linked Atrial Natriuretic Peptide Receptor Mutant Is Resistant to Desensitization.
L. R. Potter and T. Hunter (1999)
Mol. Biol. Cell 10, 1811-1820
   Abstract »    Full Text »
A Disulfide-bridged Mutant of Natriuretic Peptide Receptor-A Displays Constitutive Activity. ROLE OF RECEPTOR DIMERIZATION IN SIGNAL TRANSDUCTION.
J. Labrecque, N. Mc Nicoll, M. Marquis, and A. De Lean (1999)
J. Biol. Chem. 274, 9752-9759
   Abstract »    Full Text »    PDF »
Glomerular Mesangial Cells: Electrophysiology and Regulation of Contraction.
J. D. STOCKAND and S. C. SANSOM (1998)
Physiol Rev 78, 723-744
   Abstract »    Full Text »    PDF »
Dual Role for Adenine Nucleotides in the Regulation of the Atrial Natriuretic Peptide Receptor, Guanylyl Cyclase-A.
D. C. Foster and D. L. Garbers (1998)
J. Biol. Chem. 273, 16311-16318
   Abstract »    Full Text »    PDF »
Identification and Characterization of the Major Phosphorylation Sites of the B-type Natriuretic Peptide Receptor.
L. R. Potter and T. Hunter (1998)
J. Biol. Chem. 273, 15533-15539
   Abstract »    Full Text »    PDF »
Phosphorylation of the Kinase Homology Domain Is Essential for Activation of the A-Type Natriuretic Peptide Receptor.
L. R. Potter and T. Hunter (1998)
Mol. Cell. Biol. 18, 2164-2172
   Abstract »    Full Text »
The Cloning and Expression of a New Guanylyl Cyclase Orphan Receptor.
S. Schulz, B. J. Wedel, A. Matthews, and D. L. Garbers (1998)
J. Biol. Chem. 273, 1032-1037
   Abstract »    Full Text »    PDF »
A mutation of the atrial natriuretic peptide (guanylyl cyclase-A) receptor results in a constitutively hyperactive enzyme.
B. J. Wedel, D. C. Foster, D. E. Miller, and D. L. Garbers (1997)
PNAS 94, 459-462
   Abstract »    Full Text »    PDF »
The Photoreceptor Guanylate Cyclase Is an Autophosphorylating Protein Kinase.
J. G. Aparicio and M. L. Applebury (1996)
J. Biol. Chem. 271, 27083-27089
   Abstract »    Full Text »    PDF »
Splice Variants of Type VIII Adenylyl Cyclase.
J. J. Cali, R. S. Parekh, and J. Krupinski (1996)
J. Biol. Chem. 271, 1089-1095
   Abstract »    Full Text »    PDF »
Functional Domains of Soluble Guanylyl Cyclase.
B. Wedel, C. Harteneck, J. Foerster, A. Friebe, Gün. Schultz, and D. Koesling (1995)
J. Biol. Chem. 270, 24871-24875
   Abstract »    Full Text »    PDF »
A Variant of the alpha(2) Subunit of Soluble Guanylyl Cyclase Contains an Insert Homologous to a Region within Adenylyl Cyclases and Functions as a Dominant Negative Protein.
Sön. Behrends, C. Harteneck, Gün. Schultz, and D. Koesling (1995)
J. Biol. Chem. 270, 21109-21113
   Abstract »    Full Text »    PDF »
Two Drosophila Genes That Encode the [IMAGE] and [IMAGE] Subunits of the Brain Soluble Guanylyl Cyclase.
S. Shah and D. R. Hyde (1995)
J. Biol. Chem. 270, 15368-15376
   Abstract »    Full Text »    PDF »
Distinct Domains of the Protein Tyrosine Kinase tyk2 Required for Binding of Interferon-alpha/beta and for Signal Transduction.
L. Velazquez, K. E. Mogensen, G. Barbieri, M. Fellous, G. Uzé, and S. Pellegrini (1995)
J. Biol. Chem. 270, 3327-3334
   Abstract »    Full Text »    PDF »
Guanylyl Cyclases: Ligands and Functions.
T.D. Chrisman, S. Schulz, and D.L. Garbers (1992)
Cold Spring Harb Symp Quant Biol 57, 155-161
   Abstract »    PDF »
Activation of Protein Kinase C Stimulates the Dephosphorylation of Natriuretic Peptide Receptor-B at a Single Serine Residue. A POSSIBLE MECHANISM OF HETEROLOGOUS DESENSITIZATION.
L. R. Potter and T. Hunter (2000)
J. Biol. Chem. 275, 31099-31106
   Abstract »    Full Text »    PDF »
Agonistic Induction of a Covalent Dimer in a Mutant of Natriuretic Peptide Receptor-A Documents a Juxtamembrane Interaction That Accompanies Receptor Activation.
J. Labrecque, J. Deschenes, N. McNicoll, and A. De Lean (2001)
J. Biol. Chem. 276, 8064-8072
   Abstract »    Full Text »    PDF »
Regulation of the Atrial Natriuretic Peptide Receptor by Heat Shock Protein 90 Complexes.
R. Kumar, N. Grammatikakis, and M. Chinkers (2001)
J. Biol. Chem. 276, 11371-11375
   Abstract »    Full Text »    PDF »
Molecular and Cellular Physiology of the Dissociation of Atrial Natriuretic Peptide from Guanylyl Cyclase A Receptors.
M. A. R. Vieira, M. Gao, L. N. Nikonova, and T. Maack (2001)
J. Biol. Chem. 276, 36438-36445
   Abstract »    Full Text »    PDF »
Guanylyl Cyclase-linked Natriuretic Peptide Receptors: Structure and Regulation.
L. R. Potter and T. Hunter (2001)
J. Biol. Chem. 276, 6057-6060
   Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)