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Science 22 September 1989:
Vol. 245. no. 4924, pp. 1358 - 1365
DOI: 10.1126/science.2675311
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Articles

Science, Vol 245, Issue 4924, 1358-1365
Copyright © 1989 by American Association for the Advancement of Science

articles

Clathrin: a role in the intracellular retention of a Golgi membrane protein

GS Payne and R Schekman

Department of Biological Chemistry, UCLA School of Medicine 90024.

Yeast mutants deficient in the clathrin heavy chain secrete a precursor form of the alpha-factor, a peptide-mating pheromone. Analysis of this defect indicates that the endoprotease Kex2p, which is responsible for initiating proteolytic maturation of the alpha-factor precursor in the Golgi apparatus, is unexpectedly present at the plasma membrane in mutant cells. This result suggest that clathrin is required for the retention of Kex2p in the Golgi apparatus.


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