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Science 25 August 1989:
Vol. 245. no. 4920, pp. 852 - 855
DOI: 10.1126/science.2672333
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Articles

Science, Vol 245, Issue 4920, 852-855
Copyright © 1989 by American Association for the Advancement of Science

articles

Translational blockade imposed by cytokine-derived UA-rich sequences

V Kruys, O Marinx, G Shaw, J Deschamps, and G Huez

Departement de Biologie Moleculaire, Universite Libre de Bruxelles, Belgium.

The messenger RNAs specifying certain proteins involved in the inflammatory response and certain oncoproteins contain a conserved UA-rich sequence in the 3' untranslated region. This sequence, which is composed of several interspersed repeats of the octanucleotide UUAUUUAU, has been shown to destabilize mRNA in some eukaryotes. However, this effect is not seen when mRNAs are transferred to Xenopus oocytes, which made it possible to separate stability from translational regulation. For interferon, granulocyte-macrophage colony-stimulating factor, and c-fos RNAs, the UA-rich sequence was observed to preclude mRNA translation.


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