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Science 4 August 1989:
Vol. 245. no. 4917, pp. 525 - 528
DOI: 10.1126/science.2502844

Articles

Science, Vol 245, Issue 4917, 525-528
Copyright © 1989 by American Association for the Advancement of Science


articles

Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain

H Loosfelt, M Misrahi, M Atger, R Salesse, MT Vu Hai-Luu Thi, A Jolivet, A Guiochon-Mantel, S Sar, B Jallal, J Garnier, and al. et

Institut National de la Sante et de la Recherche Medicale Unite 135, Hopital de Bicetre, France.

Complementary DNA clones, encoding the LH-hCG (luteinizing hormone-human choriogonadotropic hormone) receptor were isolated by screening a lambda gt11 library with monoclonal antibodies. The primary structure of the protein was deduced from the DNA sequence analysis; the protein contains 696 amino acids with a putative signal peptide of 27 amino acids. Hydropathy analysis suggests the existence of seven transmembrane domains that show homology with the corresponding regions of other G protein-coupled receptors. Three other types of clones corresponding to shorter proteins were observed, in which the putative transmembrane domain was absent. These probably arose through alternative splicing. RNA blot analysis showed similar patterns in testis and ovary with a major RNA of 4700 nucleotides and several minor species. The messenger RNA was expressed in COS-7 cells, yielding a protein that bound hCG with the same affinity as the testicular receptor.


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Three Different Turkey Luteinizing Hormone Receptor (tLH-R) Isoforms I: Characterization of Alternatively Spliced tLH-R Isoforms and Their Regulated Expression in Diverse Tissues.
S. You, H. Kim, C.-C. Hsu, M. E. El Halawani, and D. N. Foster (2000)
Biol Reprod 62, 108-116
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Three Different Turkey Luteinizing Hormone Receptor (tLH-R) Isoforms II: Characterization of Differentially Regulated tLH-R Messenger Ribonucleic Acid Isoforms in the Ovary.
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Biol Reprod 62, 117-124
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Reverse Transcription-Polymerase Chain Reaction Analysis of Genes Involved in Gonadal Differentiation in Pigs.
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Biol Reprod 61, 741-748
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The {beta}-Subunit of Human Choriogonadotropin Interacts with the Exodomain of the Luteinizing Hormone/Choriogonadotropin Receptor and Changes Its Interaction with the {alpha}-Subunit.
S. Hong, I. Ji, and T. H. Ji (1999)
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The {alpha}-Subunit of Human Choriogonadotropin Interacts with the Exodomain of the Luteinizing Hormone/Choriogonadotropin Receptor.
S. Hong, I. Ji, and T. H. Ji (1999)
Endocrinology 140, 2486-2493
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S. Mukherjee, K. Palczewski, V. V. Gurevich, and M. Hunzicker-Dunn (1999)
J. Biol. Chem. 274, 12984-12989
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Intrafollicular Content of Luteinizing Hormone Receptor, {alpha}-Inhibin, and Aromatase in Relation to Follicular Growth, Estrous Cycle Stage, and Oocyte Competence for In Vitro Maturation in the Mare.
G. Goudet, F. Belin, J. Bézard, and N. Gérard (1999)
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S. Mukherjee, K. Palczewski, V. Gurevich, J. L. Benovic, J. P. Banga, and M. Hunzicker-Dunn (1999)
PNAS 96, 493-498
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M. L. G. Lamm, R. M. Rajagopalan-Gupta, and M. Hunzicker-Dunn (1999)
Endocrinology 140, 29-36
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R. M. Rajagopalan-Gupta, M. L. G. Lamm, S. Mukherjee, M. M. Rasenick, and M. Hunzicker-Dunn (1998)
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K. Ryu, R. L. Gilchrist, C.-S. Tung, I. Ji, and T. H. Ji (1998)
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T. Paukku, P. Ahtiainen, A.-M. Haavisto, and I. T. Huhtaniemi (1998)
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S. S. Stavrou, Y.-S. Zhu, L.-Q. Cai, M. D. Katz, C. Herrera, M. DeFillo-Ricart, and J. Imperato-McGinley (1998)
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S. Hong, T. Phang, I. Ji, and T. H. Ji (1998)
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The Amino-terminal Region of the Luteinizing Hormone/Choriogonadotropin Receptor Contacts Both Subunits of Human Choriogonadotropin. II. PHOTOAFFINITY LABELING.
T. Phang, G. Kundu, S. Hong, I. Ji, and T. H. Ji (1998)
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J. Bird, X. Li, Z. M. Lei, J. Sanfilippo, M. A. Yussman, and Ch. V. Rao (1998)
J. Clin. Endocrinol. Metab. 83, 1776-1782
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Modulation of High Affinity Hormone Binding. HUMAN CHORIOGONADOTROPIN BINDING TO THE EXODOMAIN OF THE RECEPTOR IS INFLUENCED BY EXOLOOP 2 OF THE RECEPTOR.
K. Ryu, H. Lee, S. Kim, J. Beauchamp, C.-S. Tung, N. W. Isaacs, I. Ji, and T. H. Ji (1998)
J. Biol. Chem. 273, 6285-6291
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Stimulating Effect of Both Human Recombinant Inhibin A and Activin A on Immature Porcine Leydig Cell Functions in Vitro.
H. Lejeune, F. Chuzel, P. Sanchez, P. Durand, J. P. Mather, and J. M. Saez (1997)
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Derivation of Functional Antagonists Using N-Terminal Extracellular Domain of Gonadotropin and Thyrotropin Receptors.
Y. Osuga, M. Kudo, A. Kaipia, B. Kobilka, and A. J. W. Hsueh (1997)
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Inhibition of Gonadotropin-Releasing Hormone Receptor Signaling by Expression of a Splice Variant of the Human Receptor.
R. Grosse, T. Schöneberg, G. Schultz, and T. Gudermann (1997)
Mol. Endocrinol. 11, 1305-1318
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Comparison of Immunocytochemical and Molecular Features with the Phenotype in a Case of Incomplete Male Pseudohermaphroditism Associated with a Mutation of the Luteinizing Hormone Receptor.
M. Misrahi, G. Meduri, S. Pissard, C. Bouvattier, I. Beau, H. Loosfelt, A. Jolivet, R. Rappaport, E. Milgrom, and P. Bougneres (1997)
J. Clin. Endocrinol. Metab. 82, 2159-2165
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Cloning and Functional Expression of the Luteinizing Hormone Receptor Complementary Deoxyribonucleic Acid from the Marmoset Monkey Testis: Absence of Sequences Encoding Exon 10 in Other Species.
F.-P. Zhang, A. S. Rannikko, P. R. Manna, H. M. Fraser, and I. T. Huhtaniemi (1997)
Endocrinology 138, 2481-2490
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Roles of Transmembrane Prolines and Proline-induced Kinks of the Lutropin/Choriogonadotropin Receptor.
S. Hong, K.-S. Ryu, M.-S. Oh, I. Ji, and T. H. Ji (1997)
J. Biol. Chem. 272, 4166-4171
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Molecular Cloning, Genomic Organization, and Developmental Regulation of a Novel Receptor from Drosophila melanogaster Structurally Related to Members of the Thyroid-stimulating Hormone, Follicle-stimulating Hormone, Luteinizing Hormone/Choriogonadotropin Receptor Family from Mammals.
F. Hauser, H.-P. Nothacker, and C. J.P. Grimmelikhuijzen (1997)
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Role of E Box and Initiator Region in the Expression of the Rat Follicle-stimulating Hormone Receptor.
T. L. Goetz, T. L. Lloyd, and M. D. Griswold (1996)
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Exoloop 3 of the Luteinizing Hormone/Choriogonadotropin Receptor.
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   Abstract »    Full Text »    PDF »
Shedding of Human Thyrotropin Receptor Ectodomain.
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