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Science 28 July 1989:
Vol. 245. no. 4916, pp. 404 - 407
DOI: 10.1126/science.2667137
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Articles

Science, Vol 245, Issue 4916, 404-407
Copyright © 1989 by American Association for the Advancement of Science

articles

The location of DNA in RecA-DNA helical filaments

EH Egelman and X Yu

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.

The helical filament that the RecA protein of Escherichia coli forms around DNA is the active apparatus in protein-catalyzed homologous genetic recombination. The actual position of DNA within this complex has been unknown. Image analysis has been performed on electron micrographs of filaments of RecA on double-stranded DNA and on single-stranded DNA to visualize a difference that is consistent with one strand of the double-stranded DNA. This localization of the DNA gives additional information about the unusual structure of DNA in the complex with RecA protein.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Inter-subunit interactions that coordinate Rad51's activities.
A. A. Grigorescu, J. H. A. Vissers, D. Ristic, Y. Z. Pigli, T. W. Lynch, C. Wyman, and P. A. Rice (2009)
Nucleic Acids Res. 37, 557-567
   Abstract »    Full Text »    PDF »
Single-molecule Chemistry and Biology Special Feature: Fueling protein DNA interactions inside porous nanocontainers.
I. Cisse, B. Okumus, C. Joo, and T. Ha (2007)
PNAS 104, 12646-12650
   Abstract »    Full Text »    PDF »
A DNA Pairing-enhanced Conformation of Bacterial RecA Proteins.
N. Haruta, X. Yu, S. Yang, E. H. Egelman, and M. M. Cox (2003)
J. Biol. Chem. 278, 52710-52723
   Abstract »    Full Text »    PDF »
A Dynamic RecA Filament Permits DNA Polymerase-catalyzed Extension of the Invading Strand in Recombination Intermediates.
L. Xu and K. J. Marians (2002)
J. Biol. Chem. 277, 14321-14328
   Abstract »    Full Text »    PDF »
Polymerization and mechanical properties of single RecA-DNA filaments.
M. Hegner, S. B. Smith, and C. Bustamante (1999)
PNAS 96, 10109-10114
   Abstract »    Full Text »    PDF »
A change in actin conformation associated with filament instability after Pi release.
L. D. Belmont, A. Orlova, D. G. Drubin, and E. H. Egelman (1999)
PNAS 96, 29-34
   Abstract »    Full Text »    PDF »
A Possible Role of the C-terminal Domain of the RecA Protein. A GATEWAY MODEL FOR DOUBLE-STRANDED DNA BINDING.
H. Kurumizaka, H. Aihara, S. Ikawa, T. Kashima, L. R. Bazemore, K. Kawasaki, A. Sarai, C. M. Radding, and T. Shibata (1996)
J. Biol. Chem. 271, 33515-33524
   Abstract »    Full Text »    PDF »
The DNA Binding Site(s) of the Escherichia coli RecA Protein.
W. M. Rehrauer and S. C. Kowalczykowski (1996)
J. Biol. Chem. 271, 11996-12002
   Abstract »    Full Text »    PDF »
Stable synapsis of homologous DNA molecules mediated by the Escherichia coli RecA protein involves local exchange of DNA strands..
K Adzuma (1992)
Genes & Dev. 6, 1679-1694
   Abstract »    PDF »


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