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Science 28 July 1989:
Vol. 245. no. 4916, pp. 396 - 399
DOI: 10.1126/science.2756426
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Articles

Science, Vol 245, Issue 4916, 396-399
Copyright © 1989 by American Association for the Advancement of Science

articles

Understanding the anomalous electrophoresis of bent DNA molecules: a reptation model

SD Levene and BH Zimm

Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093.

In polyacrylamide gel electrophoresis, the retardation of DNA molecules containing regions of intrinsic curvature can be explained by a novel reptation model that includes the elastic free energy of the DNA chain. Computer simulations based on this model give results that reproduce the dependence of anomalous mobility on gel concentration, which is quantified by new experimental data on the mobilities of circularly permuted isomers of kinetoplast DNA fragments. Fitting of the data required allowing for the elasticity of the gel.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
DNA Bending Is Essential for the Site-specific Recognition of DNA Response Elements by the DNA Binding Domain of the Tumor Suppressor Protein p53.
A. K. Nagaich, E. Appella, and R. E. Harrington (1997)
J. Biol. Chem. 272, 14842-14849
   Abstract »    Full Text »    PDF »
GATA-1 Bends DNA in a Site-independent Fashion.
R. Ghirlando and C. D. Trainor (2000)
J. Biol. Chem. 275, 28152-28156
   Abstract »    Full Text »    PDF »


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