Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 21 July 1989:
Vol. 245. no. 4915, pp. 298 - 301
DOI: 10.1126/science.2473529
Prev | Table of Contents | Next

Articles

Science, Vol 245, Issue 4915, 298-301
Copyright © 1989 by American Association for the Advancement of Science

articles

In vitro transcription enhancement by purified derivatives of the glucocorticoid receptor

LP Freedman, SK Yoshinaga, JN Vanderbilt, and KR Yamamoto

Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.

Mammalian glucocorticoid receptors enhance transcription from linked promoters by binding to glucocorticoid response element (GRE) DNA sequences. Understanding the mechanism of receptor action will require biochemical studies with purified components. Enhancement was observed in vitro with derivatives of the receptor that were expressed in Escherichia coli, purified, and added to a cell-free extract from Drosophila embryo nuclei. Transcription from promoters linked to one or multiple GREs was selectively enhanced by as much as six times. The effect was weaker with only one GRE, and enhancement was abolished by a point mutation that inactivates the GRE in vivo.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Functional Conservation of the Glutamine-Rich Domains of Yeast Gal11 and Human SRC-1 in the Transactivation of Glucocorticoid Receptor Tau 1 in Saccharomyces cerevisiae.
D.-H. Kim, G. S. Kim, C. H. Yun, and Y. C. Lee (2008)
Mol. Cell. Biol. 28, 913-925
   Abstract »    Full Text »    PDF »
Importin 7 and Importin {alpha}/Importin {beta} Are Nuclear Import Receptors for the Glucocorticoid Receptor.
N. D. Freedman and K. R. Yamamoto (2004)
Mol. Biol. Cell 15, 2276-2286
   Abstract »    Full Text »    PDF »
Heterodimerization between the Glucocorticoid Receptor and the Unrelated DNA-Binding Protein, Xenopus Glucocorticoid Receptor Accessory Factor.
B. Morin, G. R. Woodcock, L. A. Nichols, and L. J. Holland (2001)
Mol. Endocrinol. 15, 458-466
   Abstract »    Full Text »
Glucocorticoid Receptor Homodimers and Glucocorticoid-Mineralocorticoid Receptor Heterodimers Form in the Cytoplasm through Alternative Dimerization Interfaces.
J. G. A. Savory, G. G. Préfontaine, C. Lamprecht, M. Liao, R. F. Walther, Y. A. Lefebvre, and R. J. G. Haché (2001)
Mol. Cell. Biol. 21, 781-793
   Abstract »    Full Text »
Involvement of the Transcription Factor IID Protein Complex in Gene Activation by the N-Terminal Transactivation Domain of the Glucocorticoid Receptor in Vitro.
J. Ford, I. J. McEwan, A. P. H. Wright, and J.-A. Gustafsson (1997)
Mol. Endocrinol. 11, 1467-1475
   Abstract »    Full Text »
Three Amino Acid Substitutions Selectively Disrupt the Activation but Not the Repression Function of the Glucocorticoid Receptor N Terminus.
J. A. Iniguez-Lluhi, D. Y. Lou, and K. R. Yamamoto (1997)
J. Biol. Chem. 272, 4149-4156
   Abstract »    Full Text »    PDF »
Intracellular receptors use a common mechanism to interpret signaling information at response elements..
D B Starr, W Matsui, J R Thomas, and K R Yamamoto (1996)
Genes & Dev. 10, 1271-1283
   Abstract »    PDF »
Contribution of clonally distinct subpopulations to heterogeneous production of inducible nitric oxide synthase by LPS-stimulated mouse macrophages.
E.W. Alley, X. Zhang, and S.W. Russell (1994)
Innate Immunity 1, 235-242
   Abstract »    PDF »
Influence of a steroid receptor DNA-binding domain on transcriptional regulatory functions..
J A Lefstin, J R Thomas, and K R Yamamoto (1994)
Genes & Dev. 8, 2842-2856
   Abstract »    PDF »
Stereochemistry of Specific Steroid Receptor-DNA Interactions.
W. Xu, I. Alroy, L.F. Freedman, and P.B. Sigler (1993)
Cold Spring Harb Symp Quant Biol 58, 133-139
   Abstract »    PDF »
Roles of SWI1, SWI2, and SWI3 proteins for transcriptional enhancement by steroid receptors.
S. Yoshinaga, C. Peterson, I Herskowitz, and K. Yamamoto (1992)
Science 258, 1598-1604
   Abstract »    PDF »
Functional analysis of Drosophila transcription factor IIB..
S L Wampler and J T Kadonaga (1992)
Genes & Dev. 6, 1542-1552
   Abstract »    PDF »
Stimulation of transcription by an Ultrabithorax protein in vitro..
F B Johnson and M A Krasnow (1990)
Genes & Dev. 4, 1044-1052
   Abstract »    PDF »
Mutations in the glucocorticoid receptor zinc finger region that distinguish interdigitated DNA binding and transcriptional enhancement activities..
M Schena, L P Freedman, and K R Yamamoto (1989)
Genes & Dev. 3, 1590-1601
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)