Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 7 July 1989:
Vol. 245. no. 4913, pp. 71 - 74
DOI: 10.1126/science.2544999
Prev | Table of Contents | Next

Articles

Science, Vol 245, Issue 4913, 71-74
Copyright © 1989 by American Association for the Advancement of Science

articles

Rapid beta-adrenergic modulation of cardiac calcium channel currents by a fast G protein pathway

A Yatani and AM Brown

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030.

beta-Adrenergic agonists activate the G protein, Gs, which stimulates cardiac calcium currents by both cytoplasmic, indirect and membrane-delimited, direct pathways. To test whether beta-adrenergic agonists might use both pathways in the heart, isoproterenol was rapidly applied to cardiac myocytes, resulting in a biphasic increase in cardiac calcium channel currents that had time constants of 150 milliseconds and 36 seconds. beta-Adrenergic antagonists of a G protein inhibitor blocked both the fast and slow responses, whereas the adenylyl cyclase activator forskolin produced only the slow response. The presence of a fast pathway in the heart can explain what the slow pathway cannot account for: the ability of cardiac sympathetic nerves to change heart rate within a single beat.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Chronic G{alpha}s Signaling in the Striatum Increases Anxiety-Related Behaviors Independent of Developmental Effects.
C. Favilla, T. Abel, and M. P. Kelly (2008)
J. Neurosci. 28, 13952-13956
   Abstract »    Full Text »    PDF »
beta2-Adrenergic receptor agonists stimulate L-type calcium current independent of PKA in newborn rabbit ventricular myocytes.
L. P. Collis, S. Srivastava, W. A. Coetzee, and M. Artman (2007)
Am J Physiol Heart Circ Physiol 293, H2826-H2835
   Abstract »    Full Text »    PDF »
Dual effects of mesenteric lymph isolated from rats with burn injury on contractile function in rat ventricular myocytes.
A. Yatani, D.-Z. Xu, K. Irie, K. Sano, A. Jidarian, S. F. Vatner, and E. A. Deitch (2006)
Am J Physiol Heart Circ Physiol 290, H778-H785
   Abstract »    Full Text »    PDF »
Alterations of adenylyl cyclase and G proteins in aortocaval shunt-induced heart failure.
X. Wang, E. Sentex, D. Chapman, and N. S. Dhalla (2004)
Am J Physiol Heart Circ Physiol 287, H118-H125
   Abstract »    Full Text »    PDF »
Manipulating Cardiac Contractility in Heart Failure: Data From Mice and Men.
G. W. Dorn II and J. D. Molkentin (2004)
Circulation 109, 150-158
   Full Text »    PDF »
Interaction between direct sympathetic and vagus nerve stimulation on heart rate in the isolated rabbit heart.
K. E. Brack, J. H. Coote, and G A. Ng (2004)
Exp Physiol 89, 128-139
   Abstract »    Full Text »    PDF »
RGS-PX1, a GAP for Galpha s and Sorting Nexin in Vesicular Trafficking.
B. Zheng, Y.-C. Ma, R. S. Ostrom, C. Lavoie, G. N. Gill, P. A. Insel, X.-Y. Huang, and M. G. Farquhar (2001)
Science 294, 1939-1942
   Abstract »    Full Text »    PDF »
beta -Adrenergic Receptor Subtype-Specific Signaling in Cardiac Myocytes from beta 1 and beta 2 Adrenoceptor Knockout Mice.
E. Devic, Y. Xiang, D. Gould, and B. Kobilka (2001)
Mol. Pharmacol. 60, 577-583
   Abstract »    Full Text »    PDF »
Isoproterenol Activates Extracellular Signal-Regulated Protein Kinases in Cardiomyocytes Through Calcineurin.
Y. Zou, A. Yao, W. Zhu, S. Kudoh, Y. Hiroi, M. Shimoyama, H. Uozumi, O. Kohmoto, T. Takahashi, F. Shibasaki, et al. (2001)
Circulation 104, 102-108
   Abstract »    Full Text »    PDF »
Simultaneous measurements of intracellular cAMP and L-type Ca2+ current in single frog ventricular myocytes.
J.-M. Goaillard, P. Vincent, and R. Fischmeister (2001)
J. Physiol. 530, 79-91
   Abstract »    Full Text »    PDF »
Regulation of Cardiac L-Type Calcium Channels by Protein Kinase A and Protein Kinase C.
T. J. Kamp and J. W. Hell (2000)
Circ. Res. 87, 1095-1102
   Abstract »    Full Text »    PDF »
Voltage-gated calcium-channels and antiarrhythmic drug action.
A. A. Grace and A.J. Camm (2000)
Cardiovasc Res 45, 43-51
   Full Text »    PDF »
Recent Advances in Cardiac {beta}2-Adrenergic Signal Transduction.
R.-P. Xiao, H. Cheng, Y.-Y. Zhou, M. Kuschel, and E. G. Lakatta (1999)
Circ. Res. 85, 1092-1100
   Abstract »    Full Text »    PDF »
Spontaneous beta 2-Adrenergic Signaling Fails To Modulate L-Type Ca2+ Current in Mouse Ventricular Myocytes.
Y.-Y. Zhou, H. Cheng, L.-S. Song, D. Wang, E. G. Lakatta, and R.-P. Xiao (1999)
Mol. Pharmacol. 56, 485-493
   Abstract »    Full Text »
Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias.
E. Carmeliet (1999)
Physiol Rev 79, 917-1017
   Abstract »    Full Text »    PDF »
ß2-Adrenergic cAMP Signaling Is Uncoupled From Phosphorylation of Cytoplasmic Proteins in Canine Heart.
M. Kuschel, Y.-Y. Zhou, H. A. Spurgeon, S. Bartel, P. Karczewski, S.-J. Zhang, E.-G. Krause, E. G. Lakatta, and R.-P. Xiao (1999)
Circulation 99, 2458-2465
   Abstract »    Full Text »    PDF »
Rapid and Direct Effects of pH on Connexins Revealed by the Connexin46 Hemichannel Preparation.
E. Brady Trexler, F. F. Bukauskas, M. V.L. Bennett, T. A. Bargiello, and V. K. Verselis (1999)
J. Gen. Physiol. 113, 721-742
   Abstract »    Full Text »    PDF »
A Gain-of-function Polymorphism in a G-protein Coupling Domain of the Human beta 1-Adrenergic Receptor.
D. A. Mason, J. D. Moore, S. A. Green, and S. B. Liggett (1999)
J. Biol. Chem. 274, 12670-12674
   Abstract »    Full Text »    PDF »
Diazepam-binding inhibitor33-50 elicits Ca2+ oscillation and CCK secretion in STC-1 cells via L-type Ca2+ channels.
H. Yoshida, Y. Tsunoda, and C. Owyang (1999)
Am J Physiol Gastrointest Liver Physiol 276, G694-G702
   Abstract »    Full Text »    PDF »
Effects of beta 2-adrenergic stimulation on single-channel gating of rat cardiac L-type Ca2+ channels.
F. Schroder and S. Herzig (1999)
Am J Physiol Heart Circ Physiol 276, H834-H843
   Abstract »    Full Text »    PDF »
Establishment of ß-Adrenergic Modulation of L-Type Ca2+ Current in the Early Stages of Cardiomyocyte Development.
V. A. Maltsev, G. J. Ji, A. M. Wobus, B. K. Fleischmann, and J. Hescheler (1999)
Circ. Res. 84, 136-145
   Abstract »    Full Text »    PDF »
beta -Adrenergic modulation of L-type Ca2+-channel currents in early-stage embryonic mouse heart.
W. Liu, K. Yasui, A. Arai, K. Kamiya, J. Cheng, I. Kodama, and J. Toyama (1999)
Am J Physiol Heart Circ Physiol 276, H608-H613
   Abstract »    Full Text »    PDF »
Dynamics of heart rate response to sympathetic nerve stimulation.
A. Mokrane and R. Nadeau (1998)
Am J Physiol Heart Circ Physiol 275, H995-H1001
   Abstract »    Full Text »    PDF »
Alteration of intracellular Ca2+-handling and receptor regulation in hypertensive cardiac hypertrophy: insights from Ren2-transgenic rats.
O. Zolk, M. Flesch, G. Nickenig, P. Schnabel, and M. Bohm (1998)
Cardiovasc Res 39, 242-256
   Abstract »    Full Text »    PDF »
Diverse Signal Transduction Pathways Mediated by Endogenous P2 Receptors in Cultured Rat Cerebral Cortical Neurons.
T. Nishizaki and M. Mori (1998)
J Neurophysiol 79, 2513-2521
   Abstract »    Full Text »    PDF »
beta -Adrenergic regulation of constitutive nitric oxide synthase in cardiac myocytes.
A. J. Kanai, S. Mesaros, M. S. Finkel, C. V. Oddis, L. A. Birder, and T. Malinski (1997)
Am J Physiol Cell Physiol 273, C1371-C1377
   Abstract »    Full Text »    PDF »
Decreased Vasodilator Response to Isoproterenol During Nitric Oxide Inhibition in Humans.
C. Cardillo, C. M. Kilcoyne, A. A. Quyyumi, R. O. Cannon III, and J. A. Panza (1997)
Hypertension 30, 918-921
   Abstract »    Full Text »
Modulation of mEPSCs in Olfactory Bulb Mitral Cells by Metabotropic Glutamate Receptors.
N. E. Schoppa and G. L. Westbrook (1997)
J Neurophysiol 78, 1468-1475
   Abstract »    Full Text »    PDF »
Adenosine 3',5'-Cyclic Monophosphate-Stimulated Ca++ Efflux and Acetylcholine Release in Ileal Myenteric Plexus Are Mediated by N-Type Ca++ Channels: Inhibition by the Kappa Opioid Receptor Agonist.
Y. Kojima, Y. Tsunoda, and C. Owyang (1997)
J. Pharmacol. Exp. Ther. 282, 403-409
   Abstract »    Full Text »
Epoxyeicosatrienoic Acids Activate K+ Channels in Coronary Smooth Muscle Through a Guanine Nucleotide Binding Protein.
P.-L. Li and W. B. Campbell (1997)
Circ. Res. 80, 877-884
   Abstract »    Full Text »
C2 Region–Derived Peptides of ß-Protein Kinase C Regulate Cardiac Ca2+ Channels.
Z.-H. Zhang, J. A. Johnson, L. Chen, N. El-Sherif, D. Mochly-Rosen, and M. Boutjdir (1997)
Circ. Res. 80, 720-729
   Abstract »    Full Text »
Norepinephrine Induces the raf-1 Kinase/Mitogen-Activated Protein Kinase Cascade Through Both {alpha}1- and ß-Adrenoceptors.
T. Yamazaki, I. Komuro, Y. Zou, S. Kudoh, I. Shiojima, Y. Hiroi, T. Mizuno, R. Aikawa, H. Takano, and Y. Yazaki (1997)
Circulation 95, 1260-1268
   Abstract »    Full Text »
24R,25-(OH)2 Vitamin D3 Inhibits 1alpha ,25-(OH)2 Vitamin D3 and Testosterone Potentiation of Calcium Channels in Osteosarcoma Cells.
K. Takeuchi and S. E. Guggino (1996)
J. Biol. Chem. 271, 33335-33343
   Abstract »    Full Text »    PDF »
A New Cyclic AMP-independent, Gs-mediated Stimulatory Mechanism via the Adenosine A2a Receptor in the Intact Cardiac Cell.
B. T. Liang and J. F. Morley (1996)
J. Biol. Chem. 271, 18678-18685
   Abstract »    Full Text »    PDF »
Regulation of a Sodium Channel-associated G-protein by Aldosterone.
M. D. Rokaw, D. J. Benos, P. M. Palevsky, S. A. Cunningham, M. E. West, and J. P. Johnson (1996)
J. Biol. Chem. 271, 4491-4496
   Abstract »    Full Text »    PDF »
Contribution of Na+-Ca2+ Exchange to Stimulation of Transient Inward Current by Isoproterenol in Rabbit Cardiac Purkinje Fibers.
X. Han and G. R. Ferrier (1995)
Circ. Res. 76, 664-674
   Abstract »    Full Text »
ß2-Adrenergic Receptor Actions in Neonatal and Adult Rat Ventricular Myocytes.
V. Kuznetsov, E. Pak, R. B. Robinson, and S. F. Steinberg (1995)
Circ. Res. 76, 40-52
   Abstract »    Full Text »
Heart rate regulation by G proteins acting on the cardiac pacemaker channel.
A Yatani, K Okabe, J Codina, L Birnbaumer, and A. Brown (1990)
Science 249, 1163-1166
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)