Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 7 July 1989:
Vol. 245. no. 4913, pp. 68 - 71
DOI: 10.1126/science.2740916
Prev | Table of Contents | Next

Articles

Science, Vol 245, Issue 4913, 68-71
Copyright © 1989 by American Association for the Advancement of Science

articles

Histone H5 in the control of DNA synthesis and cell proliferation

JM Sun, R Wiaderkiewicz, and A Ruiz-Carrillo

Cancer Research Center, Laval University School of Medicine, L'Hotel-Dieu du Quebec, Canada.

The linker histones (H1, H5, H1 degrees) are involved in the condensation of chromatin into the 30-nanometer fiber. This supranucleosome organization correlates with the resting state of chromatin, and it is therefore possible that the linker histones play an active role in the control of chromatin activity. The effect of H5 has been directly determined by expression of an inducible transfected H5 gene in rat sarcoma cells, which do not produce H5. Transfection resulted in the reversible inhibition of DNA replication and arrest of cells in G1, at which time H5 concentrations approached that of terminally differentiated avian erythrocytes. The arrest of proliferation was accompanied by specific changes in gene expression probably related to the cell cycle block. The selectivity of these effects suggest that H5 plays an active role in the control of DNA replication and cell proliferation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
H1 Family Histones in the Nucleus: CONTROL OF BINDING AND LOCALIZATION BY THE C-TERMINAL DOMAIN.
J. P. H. Th'ng, R. Sung, M. Ye, and M. J. Hendzel (2005)
J. Biol. Chem. 280, 27809-27814
   Abstract »    Full Text »    PDF »
Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities.
E. Lukasova, Z. Koristek, M. Falk, S. Kozubek, S. Grigoryev, M. Kozubek, V. Ondrej, and I. Kroupova (2005)
J. Leukoc. Biol. 77, 100-111
   Abstract »    Full Text »    PDF »
Glom Is a Novel Mitochondrial DNA Packaging Protein in Physarum polycephalum and Causes Intense Chromatin Condensation without Suppressing DNA Functions.
N. Sasaki, H. Kuroiwa, C. Nishitani, H. Takano, T. Higashiyama, T. Kobayashi, Y. Shirai, A. Sakai, S. Kawano, K. Murakami-Murofushi, et al. (2003)
Mol. Biol. Cell 14, 4758-4769
   Abstract »    Full Text »    PDF »
Linker Histone Subtype Composition and Affinity for Chromatin in Situ in Nucleated Mature Erythrocytes.
E. Koutzamani, H. Loborg, B. Sarg, H. H. Lindner, and I. Rundquist (2002)
J. Biol. Chem. 277, 44688-44694
   Abstract »    Full Text »    PDF »
Assembly into chromatin and subtype-specific transcriptional effects of exogenous linker histones directly introduced into a living Physarum cell.
C Thiriet and J. Hayes (2001)
J. Cell Sci. 114, 965-973
   Abstract »    PDF »
Effects of H1 Histone Variant Overexpression on Chromatin Structure.
A. Gunjan, B. T. Alexander, D. B. Sittman, and D. T. Brown (1999)
J. Biol. Chem. 274, 37950-37956
   Abstract »    Full Text »    PDF »
DNA Replication in Quiescent Cell Nuclei: Regulation by the Nuclear Envelope and Chromatin Structure.
Z. H. Lu, H. Xu, and G. H. Leno (1999)
Mol. Biol. Cell 10, 4091-4106
   Abstract »    Full Text »
Histone H1 Reduces the Frequency of Initiation in Xenopus Egg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin.
Z. H. Lu, D. B. Sittman, P. Romanowski, and G. H. Leno (1998)
Mol. Biol. Cell 9, 1163-1176
   Abstract »    Full Text »
Chromatin Structure in Granulocytes. A LINK BETWEEN TIGHT COMPACTION AND ACCUMULATION OF A HETEROCHROMATIN-ASSOCIATED PROTEIN (MENT).
S. A. Grigoryev and C. L. Woodcock (1998)
J. Biol. Chem. 273, 3082-3089
   Abstract »    Full Text »    PDF »
Specific Deactivation of the Mouse Mammary Tumor Virus Long Terminal Repeat Promoter upon Continuous Hormone Treatment.
S. Boronat, H. Richard-Foy, and B. Pina (1997)
J. Biol. Chem. 272, 21803-21810
   Abstract »    Full Text »    PDF »
Histone H1 modulates DNA replication through multiple pathways in Xenopus egg extract.
Z. Lu, D. Sittman, D. Brown, R Munshi, and G. Leno (1997)
J. Cell Sci. 110, 2745-2758
   Abstract »    PDF »
Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1..
P Bouvet, S Dimitrov, and A P Wolffe (1994)
Genes & Dev. 8, 1147-1159
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)