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Science 30 June 1989:
Vol. 244. no. 4912, pp. 1582 - 1585
DOI: 10.1126/science.2500708
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Articles

Science, Vol 244, Issue 4912, 1582-1585
Copyright © 1989 by American Association for the Advancement of Science

articles

Activation of salivary secretion: coupling of cell volume and [Ca2+]i in single cells

JK Foskett and JE Melvin

Physiology Department, Armed Forces Radiobiology Research Institute, Bethesda, MD 20814.

High-resolution differential interference contrast microscopy and digital imaging of the fluorescent calcium indicator dye fura-2 were performed simultaneously in single rat salivary gland acinar cells to examine the effects of muscarinic stimulation on cell volume and cytoplasmic calcium concentration ([Ca2+]i). Agonist stimulation of fluid secretion is initially associated with a rapid tenfold increase in [Ca2+]i as well as a substantial cell shrinkage. Subsequent changes of cell volume in the continued presence of agonist are tightly coupled to dynamic levels of [Ca2+]i, even during [Ca2+]i oscillations. Experiments with Ca2+ chelators and ionophores showed that physiological elevations of [Ca2+]i are necessary and sufficient to cause changes in cell volume. The relation between [Ca2+]i and cell volume suggests that the latter reflects the secretory state of the acinar cell. Agonist-induced changes in [Ca2+]i, by modulating specific ion permeabilities, result in solute movement into or out of the cell. The resultant cell volume changes may be important in modulating salivary secretion.


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