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Science 30 June 1989:
Vol. 244. no. 4912, pp. 1571 - 1575
DOI: 10.1126/science.2544994
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Articles

Science, Vol 244, Issue 4912, 1571-1575
Copyright © 1989 by American Association for the Advancement of Science

articles

Repeat-induced G-C to A-T mutations in Neurospora

EB Cambareri, BC Jensen, E Schabtach, and EU Selker

Institute of Molecular Biology, University of Oregon, Eugene 97403.

In the Neurospora genome duplicate sequences are detected and altered in the sexual phase. Both copies of duplicate genes are inactivated at high frequency, whether or not they are linked. Restriction sites change, and affected sequences typically become heavily methylated. To characterize the alterations of the DNA, duplicated sequences were isolated before and after one or more sexual cycles. DNA sequencing and heteroduplex analyses demonstrated that the process (termed RIP) produces exclusively G-C to A-T mutations. Changes occur principally at sites where adenine is 3' of the changed cytosine. A sequence duplicated at a distant site in the genome lost approximately 10 percent of its G-C pairs in one passage through a cross. A closely linked duplication of the same sequence that was passed twice through a cross lost about half of its G-C pairs. The results suggest a mechanism for the RIP process.


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