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Science 23 June 1989:
Vol. 244. no. 4911, pp. 1475 - 1477
DOI: 10.1126/science.2499930
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Articles

Science, Vol 244, Issue 4911, 1475-1477
Copyright © 1989 by American Association for the Advancement of Science

articles

Regulatory role for GTP-binding proteins in endocytosis

LS Mayorga, R Diaz, and PD Stahl

Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110.

Guanosine 5'-triphosphate (GTP)-binding proteins have been implicated in the transport of newly synthesized proteins along the secretory pathway of yeast and mammalian cells. Early vesicle fusion events that follow receptor-mediated endocytosis as measured by three in vitro assays were blocked by guanosine 5'-O-(3-thiotriphosphate) and aluminum fluoride. The effect was specific for guanosine nucleotides and depended on the presence of cytosolic factors. Thus, GTP-binding proteins may also have a role in the transport of molecules along the endocytic pathway.


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