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Science 16 June 1989:
Vol. 244. no. 4910, pp. 1351 - 1353
DOI: 10.1126/science.2472005
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Articles

Science, Vol 244, Issue 4910, 1351-1353
Copyright © 1989 by American Association for the Advancement of Science

articles

Cl- channels in CF: lack of activation by protein kinase C and cAMP-dependent protein kinase

TC Hwang, L Lu, PL Zeitlin, DC Gruenert, R Huganir, and WB Guggino

Department of Physiology, Johns Hopkins University, Baltimore, MD 21205.

Secretory chloride channels can be activated by adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase in normal airway epithelial cells but not in cells from individuals with cystic fibrosis (CF). In excised, inside-out patches of apical membrane of normal human airway cells and airway cells from three patients with CF, the chloride channels exhibited a characteristic outwardly rectifying current-voltage relation and depolarization-induced activation. Channels from normal tissues were activated by both cAMP-dependent protein kinase and protein kinase C. However, chloride channels from CF patients could not be activated by either kinase. Thus, gating of normal epithelial chloride channels is regulated by both cAMP-dependent protein kinase and protein kinase C, and regulation by both kinases is defective in CF.


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