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Science 16 June 1989:
Vol. 244. no. 4910, pp. 1346 - 1348
DOI: 10.1126/science.2544026
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Articles

Science, Vol 244, Issue 4910, 1346-1348
Copyright © 1989 by American Association for the Advancement of Science

articles

Control of gene expression by artificial introns in Saccharomyces cerevisiae

T Yoshimatsu and F Nagawa

Institute for Biotechnology Research, Wakunaga Pharmaceutical Co., Ltd., Hiroshima, Japan.

Artificial yeast introns that show cold-sensitive splicing have been constructed. These conditional introns can be inserted into a target gene as an "intron cassette" without disrupting the coding information, allowing expression of the gene to be cold sensitive. Insertion of these intron cassettes rendered the yeast URA3 gene cold sensitive in its expression. The advantage of this intron-mediated control system is that any gene can be converted to a controllable gene by simple insertion of an intron.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Chromatin-Associated Genes Protect the Yeast Genome From Ty1 Insertional Mutagenesis.
K. M. Nyswaner, M. A. Checkley, M. Yi, R. M. Stephens, and D. J. Garfinkel (2008)
Genetics 178, 197-214
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S-Phase Checkpoint Pathways Stimulate the Mobility of the Retrovirus-Like Transposon Ty1.
M. J. Curcio, A. E. Kenny, S. Moore, D. J. Garfinkel, M. Weintraub, E. R. Gamache, and D. T. Scholes (2007)
Mol. Cell. Biol. 27, 8874-8885
   Abstract »    Full Text »    PDF »
The Effects of Mismatch Repair and RAD1 Genes on Interchromosomal Crossover Recombination in Saccharomyces cerevisiae.
A. Nicholson, R. M. Fabbri, J. W. Reeves, and G. F. Crouse (2006)
Genetics 173, 647-659
   Abstract »    Full Text »    PDF »
DNA-Directed Expression of an Animal Virus RNA for Replication-Dependent Colony Formation in Saccharomyces cerevisiae.
B. D. Price, P. Ahlquist, and L. A. Ball (2002)
J. Virol. 76, 1610-1616
   Abstract »    Full Text »    PDF »
Heat-inducible degron: a method for constructing temperature-sensitive mutants.
R. Dohmen, P Wu, and A Varshavsky (1994)
Science 263, 1273-1276
   Abstract »    PDF »
Unexpected point mutations activate cryptic 3' splice sites by perturbing a natural secondary structure within a yeast intron..
J O Deshler and J J Rossi (1991)
Genes & Dev. 5, 1252-1263
   Abstract »    PDF »


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