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Science 2 June 1989:
Vol. 244. no. 4908, pp. 1089 - 1091
DOI: 10.1126/science.2471268
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Articles

Science, Vol 244, Issue 4908, 1089-1091
Copyright © 1989 by American Association for the Advancement of Science

articles

Double-stranded ribonuclease coinduced with interferon

JM Meegan and PI Marcus

Department of Molecular and Cell Biology, The University of Connecticut, Storrs 06269.

Double-stranded (ds) RNA and many viruses are inducers of interferon (IFN), the latter presumably because they contain, or can form, dsRNA. Concomitant with the induction of IFN in chicken embryo cells was the induction of a novel double-stranded ribonuclease (dsRNase), which was released into the medium and continued to accumulate long after IFN production ceased. Only avian cells (chicken, quail, turkey, or duck) expressed high levels of this dsRNase; mammalian, turtle, or fish cells did not. Production of the nuclease was inducer dose-dependent. Optimum pH and cation requirements distinguished it from other dsRNase activities. Degradation of dsRNA was endonucleolytic. Activity resided in a molecule of an Mr of approximately 34,500. Low levels of a single-stranded (ss) RNase activity were inseparable from the dsRNase. The role for a dsRNA-inducible dsRNase released from cells is unknown.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Amelioration of Influenza Virus Pathogenesis in Chickens Attributed to the Enhanced Interferon-Inducing Capacity of a Virus with a Truncated NS1 Gene.
A. N. Cauthen, D. E. Swayne, M. J. Sekellick, P. I. Marcus, and D. L. Suarez (2007)
J. Virol. 81, 1838-1847
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Interferon Induction and/or Production and Its Suppression by Influenza A Viruses.
P. I. Marcus, J. M. Rojek, and M. J. Sekellick (2005)
J. Virol. 79, 2880-2890
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Antisense RNA: Function and Fate of Duplex RNA in Cells of Higher Eukaryotes.
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Identification and Partial Purification of Human Double Strand RNase Activity. A NOVEL TERMINATING MECHANISM FOR OLIGORIBONUCLEOTIDE ANTISENSE DRUGS.
H. Wu, A. R. MacLeod, W. F. Lima, and S. T. Crooke (1998)
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   Abstract »    Full Text »    PDF »
Regulation of ribonuclease III processing by double-helical sequence antideterminants.
K. Zhang and A. W. Nicholson (1997)
PNAS 94, 13437-13441
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